| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,126,645:1 | +C | 100% | intergenic (‑99/+50) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 91.1% | 173.1 / 11.6 | 56 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (5/0); new base C (20/31); total (25/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126500‑1126787 | gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAA‑c < 8:63824/147‑1 (MQ=255) gggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTTTTGGCCCCATAGCCT‑TCGC‑GGG‑T‑TAA‑cccgct > 7:170942/1‑145 (MQ=38) ttGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCCCGAAAGCGGAGGGATAGCCCCCCTCGCAT‑TAGCGGGG‑T‑AAC‑CCGCTCCGACACAGAGcccc > 2:255135/1‑148 (MQ=255) aaCAGGTCGACGACGCTCATTCACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTCGGCGCGGGTTTACCCGCTAAGGCGTAGTTATAGCCACCATCGCAT‑TCGC‑GGT‑T‑AAACCCGCTCCTACACAGAGCCCGcc < 4:295993/148‑1 (MQ=255) gTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTaca < 7:159914/132‑1 (MQ=255) cGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGAATGCGGAGGTATAGGCCCCATCGCAT‑TAGC‑GGG‑TAAAA‑CCGCGACAACACAGAGCCCGCCCCCCaacc > 4:77650/1‑147 (MQ=255) gAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGc < 7:103928/148‑1 (MQ=255) aaGACGCTCAGTGACATGAGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGAGGTATAGCCACCATAGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACAAAGAGCCCGCCACCCCAGCCa > 2:74044/1‑149 (MQ=38) aGACGCTCAGTCCCTTGTGGACCTCCTGCCAGGTTGTTGGGTGGACTTTTCGCCCTGTTGGCGCGGGTTTACCGGCGAATGTGGAGTTATAGCCACCATCGCTT‑TGGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGc < 4:195089/146‑1 (MQ=255) tgtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAAACCCGAATGCGGAGGTATATCCACCATCGCAT‑TCGC‑GGG‑TAAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGc > 5:63056/1‑148 (MQ=38) gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATCTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAg < 1:270428/145‑1 (MQ=255) gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGAGGTATAGCCCCCATCGCAT‑TAGC‑GGGTT‑AAC‑CCGCGCCGACACAGAGCCCGCCACCCCAGCCAGGGGGGGGGGCggaga > 2:270428/1‑145 (MQ=255) tgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTAt < 3:77650/149‑1 (MQ=255) ggACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGa > 7:247471/1‑141 (MQ=255) ggACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGa < 8:247471/141‑1 (MQ=255) ctcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGc < 1:255135/149‑1 (MQ=255) ccAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCt < 6:63056/148‑1 (MQ=255) cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCGAAATCGGAGGTATAGCCACCAAACCAT‑TACC‑GGG‑TAAAA‑CCCCACCAAAACAAAGCCCCCCCCCCCACCCAAGGGGCGGGGAGGGGAATAACGCGTccag > 6:310108/1‑146 (MQ=255) aGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACag < 7:84524/100‑1 (MQ=255) gggtgttgGGTGGACATTGCGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGGGGGCTATCAGGCGTCCTGATa < 4:261697/146‑1 (MQ=38) tggggTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctct < 5:310108/147‑1 (MQ=255) ttgcccccTGTAGGCGCGGGTTTCCCCGCGAATGCGGAGGTATAGCCACCATCCCTT‑TCGG‑GGG‑A‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACggg < 1:320237/144‑1 (MQ=255) ttGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 1:301525/131‑1 (MQ=255) cccTGTTGGCGCGGGTTTACCCGCTAAGGCGGAGGTATACCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc < 7:284529/149‑1 (MQ=38) cccTGTAGGCGCGGTTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc < 7:83570/149‑1 (MQ=255) cTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgcgc < 1:74044/149‑1 (MQ=255) cgcattcgcgGGTAAACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcga < 7:105446/127‑1 (MQ=38) cgcattcgcgGGTAAACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcga > 8:105446/7‑133 (MQ=38) ggCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCCAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcga > 7:316611/1‑129 (MQ=255) ggCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcga < 8:316611/129‑1 (MQ=255) gcgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctc < 1:208438/122‑1 (MQ=255) gcgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctc > 2:208438/1‑122 (MQ=255) tttACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg < 8:170942/149‑1 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 3:254276/106‑1 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa > 4:254276/1‑106 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt > 6:213808/1‑107 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt < 5:213808/107‑1 (MQ=255) gcgAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAg < 6:100516/107‑1 (MQ=255) gcgAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcgatc > 1:259741/1‑116 (MQ=255) gcgAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcgatc < 2:259741/116‑1 (MQ=255) gCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTg < 2:39509/134‑1 (MQ=255) gCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTg > 1:39509/1‑134 (MQ=255) gTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTc > 6:6308/1‑148 (MQ=255) gCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcga < 6:139058/97‑1 (MQ=255) gCCACCATCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcga > 5:139058/1‑97 (MQ=255) ccaccaTCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGc > 1:318331/1‑90 (MQ=255) ccaccaTCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGc < 2:318331/90‑1 (MQ=255) ccaccaTCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt < 4:68523/126‑1 (MQ=255) ccaccaTCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt > 3:68523/1‑126 (MQ=255) ccaccaTCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 6:70554/1‑149 (MQ=255) ccaccaTCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 1:183486/1‑149 (MQ=255) ccaTCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGAGCTATCAGGACGCCTGATAGCcctc > 1:159844/1‑91 (MQ=25) ccaTCGCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGAGCTATCAGGACGCCTGATAGCcctc < 2:159844/91‑1 (MQ=25) tccGCAT‑TCGC‑GGG‑T‑AAACCCGCGCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc > 7:68615/3‑149 (MQ=255) gCAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGaa > 6:321240/1‑125 (MQ=255) cAT‑TCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc > 5:178621/1‑149 (MQ=255) gg‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa > 3:41427/1‑149 (MQ=255) gg‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa < 2:183486/149‑1 (MQ=255) | GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCAT‑TCGC‑GGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126500‑1126787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |