Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,355,474:1 +T 5.6% coding (5/264 nt) PP_1180 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,355,4741.T 5.6% 293.2 / 9.5 71coding (5/264 nt)PP_1180hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (34/33);  new base T (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAA  >  NC_002947/1355329‑1355616
                                                                                                                                                    |                                                                                                                                              
cTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAatgca‑tgc                                                                                                                                             <  5:213400/149‑1 (MQ=255)
     gCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATc                                                                                                                                        >  7:214820/1‑149 (MQ=255)
           gggCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTAAGGCGCCTTATTGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATccgcccg                                                                                                                                  >  7:92886/1‑149 (MQ=255)
               cTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGCGGATGCGC‑ATGCATTGCGCATccccgcgag                                                                                                                                <  7:128660/149‑8 (MQ=38)
               cTGCGGGTTCTCGCGAGTCGTGTCGGGTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGCGGATGCGC‑ATGCATTGCGCATccccgcgag                                                                                                                                >  8:128660/1‑142 (MQ=38)
                tGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCgg                                                                                                                             >  5:111854/1‑149 (MQ=255)
                      ttCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTAc                                                                                                                       <  4:252260/149‑1 (MQ=255)
                        ctcGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTAcc                                                                                                                      <  7:264964/148‑1 (MQ=255)
                             gAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTc                                                                                                                >  1:63965/1‑149 (MQ=255)
                               gTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCgg                                                                                                              <  6:111854/149‑1 (MQ=255)
                                tCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCgg                                                                                                              <  8:214820/148‑1 (MQ=255)
                                  gtgtCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGa                                                                                                            >  5:282553/1‑148 (MQ=255)
                                  gtgtCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGAc                                                                                                           <  6:265152/149‑1 (MQ=255)
                                  gtgtCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGAAAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGa                                                                                                            <  3:334903/148‑1 (MQ=255)
                                   tgtCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATATCCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCTTGCGCCCGCGCGGGCGTACCGACTTCGGGACa                                                                                                          <  1:32921/149‑1 (MQ=255)
                                    gtCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATccgccc                                                                                                                                   <  8:86269/123‑1 (MQ=255)
                                    gtCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATccgccc                                                                                                                                   >  7:86269/1‑123 (MQ=255)
                                       ggTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACa                                                                                                          >  8:136868/1‑145 (MQ=255)
                                       ggTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACa                                                                                                          <  7:136868/145‑1 (MQ=255)
                                       ggTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACt                                                                                                      >  8:139076/1‑149 (MQ=255)
                                        gTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGAAATGCA‑TGCGCATCCGCCCCCGCGGGCGTACCGACTTCGGGACAGACt                                                                                                      >  2:96028/1‑148 (MQ=255)
                                         tttGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCa                                                                                                    <  7:139076/149‑1 (MQ=255)
                                             cATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGaa                                                                                                >  8:228196/1‑149 (MQ=255)
                                                  gtgggtgTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAgg                                                                                           <  2:63965/149‑1 (MQ=255)
                                                      gtgtCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGc                                                                                        >  2:176331/1‑148 (MQ=255)
                                                          ctcCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATgg                                                                                   >  4:58325/1‑149 (MQ=255)
                                                            ccACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCt                                                                                 >  1:336832/1‑149 (MQ=255)
                                                             cacaGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCtt                                                                                >  5:226231/1‑149 (MQ=255)
                                                              acaGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTg                                                                               <  8:92886/149‑1 (MQ=255)
                                                                aGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGcc                                                                             <  2:336832/149‑1 (MQ=255)
                                                                  tttCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGc                                                                           >  5:107762/1‑149 (MQ=255)
                                                                    tCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTc                                                                         >  7:129721/1‑149 (MQ=255)
                                                                    tCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTc                                                                         >  1:33580/1‑149 (MQ=255)
                                                                       atatTTTACAGGCGCCATATGGGAGCCCAACCTTCAGTCCAATAGCTCGATAACATCGCGC‑‑GGATGCGCAATCCA‑TGAGCATCCGCCCGCGCGCGCGTACCGACTTCGGGACAGACACAGGAAGAAGCGGAAATGACTTGCCGCTCCCt                                                                      >  6:276892/1‑149 (MQ=255)
                                                                        tatTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTg                                                                     >  8:206402/1‑149 (MQ=255)
                                                                         atTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTgg                                                                    >  6:248237/1‑149 (MQ=255)
                                                                          ttGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCTCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTgg                                                                    <  7:228196/148‑1 (MQ=255)
                                                                            gTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGcc                                                                  >  3:271737/1‑148 (MQ=255)
                                                                                   cgcCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCg                                                                                                               <  2:65810/96‑1 (MQ=255)
                                                                                   cgcCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCg                                                                                                               >  1:65810/1‑96 (MQ=255)
                                                                                         aTGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTc                                                    >  1:182100/1‑149 (MQ=255)
                                                                                           gggCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAg                                                                                                   >  6:114875/1‑100 (MQ=255)
                                                                                           gggCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAg                                                                                                   <  5:114875/100‑1 (MQ=255)
                                                                                            ggCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GTATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCTACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTgg                                                 <  1:96028/149‑1 (MQ=255)
                                                                                             gcgcCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTggg                                                <  8:129721/149‑1 (MQ=255)
                                                                                                     cGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGccc                                        <  2:182100/149‑1 (MQ=255)
                                                                                                           gTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAAc                                  >  2:49965/1‑149 (MQ=255)
                                                                                                              cACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTg                                <  5:248237/148‑1 (MQ=255)
                                                                                                               aCTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGtt                              >  7:301345/1‑149 (MQ=255)
                                                                                                                  tGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTgg                                                                    >  6:31145/1‑108 (MQ=255)
                                                                                                                  tGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTgg                                                                    <  5:31145/108‑1 (MQ=255)
                                                                                                                       gATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGc                                                                              >  7:28766/1‑93 (MQ=255)
                                                                                                                       gATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGc                                                                              <  8:28766/93‑1 (MQ=255)
                                                                                                                       gATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATgg                      <  4:271737/149‑1 (MQ=255)
                                                                                                                            ccTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGc                  >  2:249754/1‑148 (MQ=255)
                                                                                                                             cTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAgggg                                                                                         <  4:212904/76‑1 (MQ=255)
                                                                                                                             cTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAgggg                                                                                         >  3:212904/1‑76 (MQ=255)
                                                                                                                                gcgG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTggg                                                >  6:583/1‑114 (MQ=255)
                                                                                                                                gcgG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTggg                                                <  5:583/114‑1 (MQ=255)
                                                                                                                                   g‑‑ggATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctg          <  6:282553/149‑1 (MQ=255)
                                                                                                                                   g‑‑ggATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctg          <  1:176331/149‑1 (MQ=255)
                                                                                                                                    gcggATGCGC‑ATGCATTGCGCATCC‑CCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAAc                                  >  1:210618/3‑125 (MQ=255)
                                                                                                                                    gcggATGCGC‑ATGCATTGCGCATCC‑CCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAAc                                  <  2:210618/123‑1 (MQ=255)
                                                                                                                                       gATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGtt                              >  3:35606/1‑127 (MQ=255)
                                                                                                                                       gATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGtt                              <  4:35606/127‑1 (MQ=255)
                                                                                                                                           cgcAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctg          <  8:192517/143‑1 (MQ=255)
                                                                                                                                           cgcAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctg          >  7:192517/1‑143 (MQ=255)
                                                                                                                                           cgcAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGc    <  1:49965/149‑1 (MQ=255)
                                                                                                                                             cAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCaa  <  2:33580/149‑1 (MQ=255)
                                                                                                                                             cAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCaa  <  6:107762/149‑1 (MQ=255)
                                                                                                                                             cAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCaa  <  6:226231/149‑1 (MQ=255)
                                                                                                                                              aaTGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTgg                                                 >  6:171582/1‑101 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAA  >  NC_002947/1355329‑1355616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑