Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,915,598:1	+A	9.5%	intergenic (+26/‑263)	fklB‑II → / → PP_1715	FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,915,598	1	.	A	9.5%	234.1 / 19.7	63	intergenic (+26/‑263)	fklB‑II/PP_1715	FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (26/31);  new base A (3/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

GCTGATCCCGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAA  >  NC_002947/1915452‑1915745                                                                                                                                                    |                                                                                                                                                    gCTGATCCCGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCc‑a                                                                                                                                                    >  8:99098/1‑148 (MQ=255)        ccGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACAttgtt                                                                                                                                             <  7:99098/148‑1 (MQ=255)          ggCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTggg                                                                                                                                          <  2:173542/149‑1 (MQ=255)             cAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACTCCCA‑ACAATGTTGGGGCg                                                                                                                                       >  8:316941/1‑149 (MQ=255)              aaCTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGt                                                                                                                                      >  6:76143/1‑149 (MQ=255)                    gTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGt                                                                                                                                <  2:176657/149‑1 (MQ=255)                       cTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGttt                                                                                                                              <  1:309557/148‑1 (MQ=255)                          gttttCGATCTGGAACTTAGCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTtgt                                                                                                                            <  8:160003/144‑1 (MQ=255)                          gtgtTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTtgtgt                                                                                                                          >  4:173073/1‑148 (MQ=255)                               cGATCTGGAACTGATCGCCATCAAGGACCCGG‑CCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTtgt                                                                                                                            <  1:253289/141‑1 (MQ=255)                               cGATCTGGAACTGATCGCCATCAAGGACCCGG‑CCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTtgt                                                                                                                            >  2:253289/1‑141 (MQ=255)                                gATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCGC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTg                                                                                                                    >  7:1445/1‑149 (MQ=255)                                gATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGAtt                                                                                                                     <  5:76143/148‑1 (MQ=255)                                gATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTg                                                                                                                    >  1:199626/1‑149 (MQ=255)                                     ggAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACg                                                                                                                >  2:316266/1‑148 (MQ=255)                                      gAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTgg                                                                                                                   <  6:185054/144‑1 (MQ=255)                                          tGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACAttgtt                                                                                                                                             <  8:182066/114‑1 (MQ=255)                                          tGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACAttgtt                                                                                                                                             >  7:182066/1‑114 (MQ=255)                                              cGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGTCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCaa                                                                                                       <  7:126803/148‑1 (MQ=255)                                                ccATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGttt                                                                                                                              <  6:156477/123‑1 (MQ=255)                                                ccATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGttt                                                                                                                              >  5:156477/1‑123 (MQ=255)                                                  aTCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCt                                                                                                  <  8:186198/149‑1 (MQ=255)                                                      aGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATa                                                                                              >  8:108240/1‑149 (MQ=255)                                                       ggACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATAc                                                                                             >  1:52013/1‑149 (MQ=255)                                                          cccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGt                                                                                          <  2:52013/149‑1 (MQ=255)                                                           ccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGttt                                                                                                                              >  3:32947/1‑112 (MQ=255)                                                           ccGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGttt                                                                                                                              <  4:32947/112‑1 (MQ=255)                                                             ggCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTcc                                                                                        >  6:78296/1‑148 (MQ=255)                                                              gCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGa                                                                                      <  1:316266/149‑1 (MQ=255)                                                                   gTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACTCCCC‑ACATTGTTGGGGAGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGaaa                                                                                 <  7:31167/149‑1 (MQ=255)                                                                        aaGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAg                                                                            >  7:190367/1‑149 (MQ=255)                                                                            tGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTg                                                                        <  8:190367/149‑1 (MQ=255)                                                                              ccGAGCCGCAGGACGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaa                                                                      <  7:316941/149‑1 (MQ=255)                                                                                gAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaag                                                                     >  7:198824/1‑148 (MQ=255)                                                                                gAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaag                                                                     <  8:198824/148‑1 (MQ=255)                                                                                 aGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaagaa                                                                   <  5:78296/149‑1 (MQ=255)                                                                                        ggCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGAt                                                            <  4:200560/149‑1 (MQ=255)                                                                                           cGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGtt                                                         <  8:1445/149‑1 (MQ=255)                                                                                           cGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGtt                                                         <  2:199626/149‑1 (MQ=255)                                                                                                  ccTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACg                                                  >  2:281551/1‑149 (MQ=255)                                                                                                  ccTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACg                                                  >  6:239868/1‑149 (MQ=255)                                                                                                      aagaagCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCaaa                                              >  4:53566/1‑149 (MQ=255)                                                                                                         aagCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCg                                           >  2:248190/1‑149 (MQ=255)                                                                                                            cACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGACCAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGttt                                        <  1:248190/149‑1 (MQ=255)                                                                                                               cTGCCAAGTAACGTTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaagaa                                                                   <  8:37621/119‑1 (MQ=255)                                                                                                               cTGCCAAGTAACGTTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTgaagaa                                                                   >  7:37621/1‑119 (MQ=255)                                                                                                                  ccAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGcgccg                                  >  7:87715/1‑149 (MQ=255)                                                                                                                  ccAAGTAACATTGGCTGGTTGTCGCAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACATGCAACAGCTCATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGATTCTGACGCAAATCGTTTGcgccg                                  >  2:323217/1‑149 (MQ=255)                                                                                                                    aaGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGcgccg                                  >  5:10134/1‑147 (MQ=255)                                                                                                                     aGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAg                               <  8:87715/149‑1 (MQ=255)                                                                                                                      gTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTTAAGAAGTAGGAAGTTTCTGACGCAAATCGATTGCGCCGCAgg                              >  4:100627/1‑149 (MQ=255)                                                                                                                           aTTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTc                         <  5:239868/149‑1 (MQ=255)                                                                                                                                     aaacgaaaaCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAg                               >  6:17776/7‑133 (MQ=39)                                                                                                                                     aaacgaaaaCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAg                               <  5:17776/127‑1 (MQ=39)                                                                                                                                      gTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGaa               <  7:108240/148‑1 (MQ=255)                                                                                                                                       tCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCTAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAAc             <  3:100627/149‑1 (MQ=255)                                                                                                                                         ggAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGa           >  2:123599/1‑149 (MQ=255)                                                                                                                                         gaaaaCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTc                                                       <  6:346402/103‑1 (MQ=38)                                                                                                                                         gaaaaCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTc                                                       >  5:346402/3‑105 (MQ=38)                                                                                                                                         gaaaaCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGc                    >  2:240412/3‑140 (MQ=255)                                                                                                                                         gaaaaCGCCCCAACAATG‑TGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGc                    <  1:240412/138‑1 (MQ=255)                                                                                                                                          gAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGAc          <  1:281551/149‑1 (MQ=255)                                                                                                                                           aaaCGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACt         <  6:10134/149‑1 (MQ=255)                                                                                                                                               gCCCC‑ACATTGTTGGGGCGTTTTCGTTGGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACtgtg      >  1:10289/1‑148 (MQ=255)                                                                                                                                                   c‑acaTTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTaaa  >  1:97491/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCTGATCCCGGCCAACTCGGTACTGGTGTTCGATCTGGAACTGATCGCCATCAAGGACCCGGCCCAGTTGCAAGGTGCCGAGCCGCAGGCCGAAGCCCCTGAAGAAGCACCTGCCAAGTAACATTGGCTGGTTGTCGGAAACGCCCC‑ACATTGTTGGGGCGTTTTCGTTTGTGCCGATTGGACGAACCTGCAACAGCTGATACTGTCCGAGGAAATGCAGCGTGAAGAAGTAGGATGTTTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAA  >  NC_002947/1915452‑1915745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑