Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,916,845:1 +C 8.0% coding (98/369 nt) PP_1717 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,916,8451.C 8.0% 188.3 / 11.4 50coding (98/369 nt)PP_1717hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (21/25);  new base C (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GCCGCGCCCTGGCCTGGCATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTGCGCAGTTCGCTGGTGATCAGCCAAGGCGCCAGCCG  >  NC_002947/1916699‑1916977
                                                                                                                                                   |                                                                                                                                    
gccgcGCCCTGGCCTGGCATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCTGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑Ga                                                                                                                                    >  6:74044/1‑149 (MQ=255)
         tggcctggcATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCAGTCGTCAACGTCACTTGCTGAGATACCGAGCGCT‑GATGGAGCGCt                                                                                                                           >  6:295523/1‑149 (MQ=255)
           gcgtggcATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGGCAGGTCAGGAGCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCTCGATC‑AGCGCtcg                                                                                                                         <  3:277302/146‑3 (MQ=255)
             ctggcATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCTCGATC‑AGCGCtcgg                                                                                                                        >  4:238386/1‑145 (MQ=255)
             ctggcATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCTCGATC‑AGCGCtcgg                                                                                                                        >  3:69854/1‑145 (MQ=255)
             ctggcATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCa                                                                                                                       <  5:8958/149‑1 (MQ=255)
                gcATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCTCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGc                                                                                                                     <  5:290710/148‑1 (MQ=255)
                gcATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCTCGATC‑AGCGCtcggta                                                                                                                      <  3:238386/147‑6 (MQ=255)
                      gggggTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCtt                                                                                                                          <  8:99437/137‑1 (MQ=255)
                      gggggTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCtt                                                                                                                          >  7:99437/1‑137 (MQ=255)
                        gggTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGcc                                                                                                            >  7:103151/1‑149 (MQ=255)
                           tggctggcTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCgg                                                                                                         <  8:103151/149‑1 (MQ=255)
                                 gcttgAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTGGTCAACGTCACTTTCTGACATACCGAGCGCT‑GATCGTGGGCTTGCAGCTACCCAGCCCGGCCATg                                                                                                    <  1:138621/148‑1 (MQ=255)
                                 gctggAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCTTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCTGCCATGc                                                                                                   <  3:28598/149‑1 (MQ=255)
                                     gagcgagcCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGTGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGAc                                                                                               <  3:52952/149‑1 (MQ=255)
                                          agcCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGa                                                                                          >  8:51076/1‑149 (MQ=255)
                                             catcatGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTg                                                                                       >  7:243796/1‑149 (MQ=255)
                                              atcatGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGc                                                                                      >  2:70407/1‑149 (MQ=255)
                                                 atGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCcgc                                                                                   <  1:70407/149‑1 (MQ=255)
                                                     gCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCt                                                                                <  3:84919/148‑1 (MQ=255)
                                                     gCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCt                                                                                >  4:84919/1‑148 (MQ=255)
                                                     gCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTg                                                                               <  1:283182/149‑1 (MQ=255)
                                                     gCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTg                                                                               <  2:7090/149‑1 (MQ=255)
                                                         ggTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAAt                                                                           <  7:51076/149‑1 (MQ=255)
                                                           tCAGGATCTGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCAGCCATGCAGACGCTGACTGCCGCGCTGGAATAc                                                                         <  5:74044/149‑1 (MQ=255)
                                                              ggATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTg                                                                      >  6:122379/1‑149 (MQ=255)
                                                                    gCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTg                                                                >  8:247383/1‑149 (MQ=255)
                                                                    gCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTg                                                                <  5:122379/149‑1 (MQ=255)
                                                                          aGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGAt                                                          <  2:132161/149‑1 (MQ=255)
                                                                                  gATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTg                                                             <  5:118352/138‑1 (MQ=255)
                                                                                  gATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTg                                                             >  6:118352/1‑138 (MQ=255)
                                                                                                 gAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTgcg                                   >  4:333054/1‑149 (MQ=255)
                                                                                                   gCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTGCGCa                                 <  2:18471/149‑1 (MQ=255)
                                                                                                       gACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGAt                                                          >  4:154173/1‑120 (MQ=255)
                                                                                                       gACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGAt                                                          <  3:154173/120‑1 (MQ=255)
                                                                                                             gCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTGCGCAGTTCGCTgg                        <  3:137936/148‑1 (MQ=255)
                                                                                                                  tcgtcAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGcc                                                 <  2:109502/118‑1 (MQ=255)
                                                                                                                  tcgtcAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGcc                                                 >  1:109502/1‑118 (MQ=255)
                                                                                                                   cgtcAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTGCGCAGTTCGCTGGTGATCAg                 >  5:107946/1‑149 (MQ=255)
                                                                                                                       aaCGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGc                                                  <  4:125216/112‑1 (MQ=255)
                                                                                                                       aaCGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGc                                                  >  3:125216/1‑112 (MQ=255)
                                                                                                                        aCGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCAGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTGCGCAGTTCGCTGGTGATCAGCCAAg            >  3:134626/1‑149 (MQ=255)
                                                                                                                          gTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTGCGCAGTTCGCTGGTGATCAGCCAAGgc          <  3:333054/149‑1 (MQ=255)
                                                                                                                              cTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGAt                                                          <  2:242940/97‑1 (MQ=255)
                                                                                                                              cTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGAt                                                          >  1:242940/1‑97 (MQ=255)
                                                                                                                               ttGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCACAGCCCGGCCATGCATACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATTACCGTGCCGAGCCGCCATTGAGCAGTTCGATGGTGATAAGACAAGGAGCCAg     >  1:97802/1‑149 (MQ=255)
                                                                                                                                tGCTGACATACCGAGCGCT‑GATCGAGCGCATGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCGGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTGCGCAGTTCGCTGGTGATCAGCCAAGGCGCCAGc    >  6:263704/1‑149 (MQ=255)
                                                                                                                                 gCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCACGCAGACGCTGACTGCCGCGCTGGAATACCt                                                                       >  8:190826/1‑81 (MQ=255)
                                                                                                                                  cTGACATAGCGAGCGCT‑GATCGAGCGCTTGCAGCTGCCCAGCCGGGGCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTGCGCAGTTCGCTGGTGATCAGCCAAGGCGCCAGCCg  <  4:134626/149‑1 (MQ=255)
                                                                                                                                       aTACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTg                                     <  2:289571/109‑1 (MQ=255)
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GCCGCGCCCTGGCCTGGCATCTGGGGGTGGCTGGCTGGAGCGAGCCATCATGAGCCAGGTCAGGATCAGCCGTCAGCACTGGGATAACCTGCTGGGCGAGCTTGACCTGGCCCGTCGTCAACGTCACTTGCTGACATACCGAGCGCT‑GATCGAGCGCTTGCAGCTCCCCAGCCCGGCCATGCAGACGCTGACTGCCGCGCTGGAATACCTGGCGGTGCTGGATGCCCGTGCCGAGCAGCCATTGCGCAGTTCGCTGGTGATCAGCCAAGGCGCCAGCCG  >  NC_002947/1916699‑1916977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑