New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 678543	51 (0.970)	3 (0.060)	3/240	NT	6.4%	coding (475/1374 nt)	PP_0581	3‑oxoacyl‑ACP reductase
	?	NC_002947	= 678580	39 (0.780)					coding (438/1374 nt)	PP_0581	3‑oxoacyl‑ACP reductase

ACTCTTTGGCGAGAGGAAGAAGCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACAAGTGACTGGCATCG  >  NC_002947/678436‑678721                                                                                                                                                  |                                                                                                                                                tgaagcagttgcgtgaatttttccagccgctgttacgcagcctggccccatgcgcccatgtactggtgctgggccgtgcaccggaaggcctcgacgacccacTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTg                                                                                                                                              <  7:200049‑M1/47‑1 (MQ=255) aCTCTTTGGCGAGAGGAAGAAGCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGctgg                                                                                                                                             <  4:79242‑M2/149‑5 (MQ=255)   tctTTGGCGAGAGGAAGAAGCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGctggc                                                                                                                                            <  3:124638‑M2/148‑6 (MQ=255)    agcagttgcgggaatttttccagccgctgttacgcagcctggccccatgcgcccatgtactggtgctgggccgtgcaccggaaggcctcgacgacccacTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGcc                                                                                                                                           >  3:187450‑M1/100‑149 (MQ=255)     tttGGCGAGAGGAAGAAGCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACg                                                                                                                                                 >  1:130499/1‑141 (MQ=255)     tttGGCGAGAGGAAGAAGCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACg                                                                                                                                                 <  2:130499/141‑1 (MQ=255)      cagttgcgtgaatttttccagccgctgttacgcagcctggccccatgcgcccatgtactggtgctgggccgtgcaccggaaggcctcgacgacccacTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAg                                                                                                                                         >  8:200049‑M1/98‑149 (MQ=255)                gaagaaGCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGgtcgtc                                                                                                                                >  6:333394/1‑147 (MQ=255)                 aagaagCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGTGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAg                                                                                                                             >  7:255503/1‑149 (MQ=255)                  agaagCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAgg                                                                                                                            >  7:24954/1‑149 (MQ=255)                   gaagCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGc                                                                                                                           >  1:12206/1‑149 (MQ=255)                        gcAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGTAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGctg                                                                                                                                              <  1:185184‑M2/125‑4 (MQ=255)                        gcAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGTAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGctg                                                                                                                                              >  2:185184‑M2/1‑122 (MQ=255)                         cAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCg                                                                                                                     <  5:220235/149‑1 (MQ=255)                          aGGGCGCCTTCCAGCTGATCTTCGGCACC‑GGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGt                                                                                                                   >  6:288292/1‑149 (MQ=255)                            ggCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTg                                                                                                                  <  5:333394/149‑1 (MQ=255)                                    ccAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGcc                                                                                                           >  7:32654/1‑148 (MQ=255)                                     cAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCa                                                                                                         <  8:32654/149‑1 (MQ=255)                                                  gCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATg                                                                                          <  2:12206/149‑1 (MQ=255)                                                   cACCGGTGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATgg                                                                                         <  8:255503/149‑1 (MQ=255)                                                   cACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATgg                                                                                         <  3:149108/149‑1 (MQ=255)                                                     ccGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGgc                                                                                       <  4:188569/149‑1 (MQ=255)                                                       gggggCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGgcgc                                                                                     <  4:266619/149‑1 (MQ=255)                                                       gggggCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGgcg                                                                                      >  3:266619/1‑148 (MQ=255)                                                         gggCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCAt                                                                                   <  3:79242/149‑1 (MQ=255)                                                              aCATACAGCAGCTGGGCAGTAGCGCCATTGCGCAGTTTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATgggg                                                                               >  1:232563/1‑149 (MQ=255)                                                                   cagcagCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGc                                                                         >  5:72029/1‑149 (MQ=255)                                                                      cagcTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGc                                                                      <  4:124638/149‑1 (MQ=255)                                                                       agcTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGc                                                                      <  2:187787/148‑1 (MQ=255)                                                                       agcTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCg                                                                     <  6:330490/149‑1 (MQ=255)                                                                                gTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGctg                                                                                                                                              >  8:91024‑M2/1‑66 (MQ=255)                                                                                gTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGctg                                                                                                                                              <  7:91024‑M2/69‑4 (MQ=255)                                                                                     gcCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTgg                                                        >  4:295977/1‑148 (MQ=255)                                                                                        aTTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTa                                                                                              >  4:304075/1‑107 (MQ=255)                                                                                        aTTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTa                                                                                              <  3:304075/107‑1 (MQ=255)                                                                                        aTTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGaaaa                                                    <  3:295977/149‑1 (MQ=255)                                                                                            cgcAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGTCAGGCTGCGTAACAGCGGCTGGAAAAATTc                                                <  2:232563/149‑1 (MQ=255)                                                                                               aG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACg                                             >  8:333444/1‑149 (MQ=255)                                                                                                      ttGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGctggccaaggaactgcgcaatggcgctactgcccagctgctgtatgtcgcccccggtgccgaagatcagctggaaggcgccctgcgcttcttcctctcgcca                                           <  6:99568‑M2/147‑103 (MQ=255)                                                                                                       tGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGctggccaaggaactgcgcaatggcgctactgcccagctgctgtatgtcgcccccggtgccgaagatcagctggaaggcgccctgcgcttcttcctctcgc                                             <  3:9484‑M2/144‑101 (MQ=255)                                                                                                       tGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGctggccaaggaactgcgcaatggcgctactgcccagctgctgtatgtcgcccccggtgccgaagatcagctggaaggcgccctgcgcttcttcctctcgc                                             >  4:9484‑M2/1‑44 (MQ=255)                                                                                                        GGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCCCTGCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACt                                        >  1:104562‑M1/1‑148 (MQ=255)                                                                                                            AGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAg                                 <  8:197987‑M1/149‑1 (MQ=255)                                                                                                                        aaGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTc                     >  7:43286/1‑149 (MQ=255)                                                                                                                             cTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGt                                >  2:167312/1‑133 (MQ=255)                                                                                                                             cTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGt                                <  1:167312/133‑1 (MQ=255)                                                                                                                             cTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTTCATCGCGGTCGTAc                 >  8:146671/1‑148 (MQ=255)                                                                                                                             cTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACa                <  7:197987/149‑1 (MQ=255)                                                                                                                                     cACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACAAGTGACTg        <  7:333444/149‑1 (MQ=255)                                                                                                                                     cACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACAAGTGACTg        <  6:72029/149‑1 (MQ=255)                                                                                                                                         cTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACAAGTGACTGGCAt    <  8:43286/149‑1 (MQ=255)                                                                                                                                          tgtgCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACAAGTGACTGGCATc   >  8:46398/1‑149 (MQ=255)                                                                                                                                            tgCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACAAGTGACTGGCATc   <  7:146671/147‑1 (MQ=255)                                                                                                                                            tgCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACAAGTGACTGGCATCg  >  4:158177/1‑148 (MQ=255)                                                                                                                                            tgCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACAAGTGACTGGCATCg  >  4:152964/1‑148 (MQ=255)                                                                                                                                                  gCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCAc                                              >  3:147500/1‑98 (MQ=255)                                                                                                                                                  gCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCAc                                              <  4:147500/98‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                ACTCTTTGGCGAGAGGAAGAAGCGCAGGGCGCCTTCCAGCTGATCTTCGGCACCGGGGGCGACATACAGCAGCTGGGCAGTAGCGCCATTGCGCAG‑TTCCTTGGCCAGTGAGCGGCTGAAGCCCTCAAGGGCACGCTGTGCAACGCTGGCCAGTGGGTCGTCGAGGCCTTCCGGTGCACGGCCCA‑‑GCACCAGTACATGGGCGCATGGGGCCAGGCTGCGTAACAGCGGCTGGAAAAATTCACGCAACTGCTTCAGTGCATCGCTGTCGGACAAGTGACTGGCATCG  >  NC_002947/678436‑678721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.