Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,973,055 G→C 8.0% F273L (TTC→TTG)  PP_2600 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,973,0550GC 8.0% 157.8 / 4.2 50F273L (TTC→TTG) PP_2600hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/22);  new base C (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GACTTCAGGTCGGTGGCAACCACGCCGCCGGCGGACTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGCTTGCAGGTGCACACCCAGGT  >  NC_002947/2972909‑2973197
                                                                                                                                                  |                                                                                                                                              
gACTTCAGGTCGGTGGCAACCACGCCGCCGGCGGACTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGa                                                                                                                                               >  6:275182/1‑148 (MQ=255)
       ggTCGGTGGCAACCACGCCGCCGGCGGACTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCt                                                                                                                                       <  5:275182/149‑1 (MQ=255)
       ggTCGGTGGCAACCACGCCGCCGGCGGACTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCt                                                                                                                                       <  5:120403/149‑1 (MQ=255)
        gTCGGTGGCAACCACGCCGCCGGCGGACTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTg                                                                                                                                      >  5:267587/1‑149 (MQ=255)
         tCGGTGGCAACCACGCCGCCGGCGGACTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTgc                                                                                                                                     >  5:317496/1‑149 (MQ=255)
                         cgccgGCGGACTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGcc                                                                                                                     <  5:302705/149‑1 (MQ=255)
                         cgccgGCGGACTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGcc                                                                                                                     <  3:39103/149‑1 (MQ=255)
                                   cTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGa                                                                                                           <  6:72500/149‑1 (MQ=255)
                                      gCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCgg                                                                                                            >  5:64475/1‑145 (MQ=255)
                                      gCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCgg                                                                                                            <  6:64475/145‑1 (MQ=255)
                                        ccAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGa                                                                                                           >  4:79946/1‑144 (MQ=255)
                                        ccAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGa                                                                                                           <  3:79946/144‑1 (MQ=255)
                                        ccAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAgc                                                                                                      >  6:158842/1‑149 (MQ=255)
                                            ccGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAgctgct                                                                                                  <  6:267587/149‑1 (MQ=255)
                                                     aTCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCtgtg                                                                                         <  3:312070/149‑1 (MQ=255)
                                                          cAGAACGCCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGCAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCtgt                                                                                          <  7:114593/143‑1 (MQ=255)
                                                          cAGAACGCCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGCAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCtgt                                                                                          >  8:114593/1‑143 (MQ=255)
                                                          cAGAACGCCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGCAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCtgt                                                                                          >  8:119515/1‑143 (MQ=255)
                                                          cAGAACGCCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGCAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCtgt                                                                                          <  7:119515/143‑1 (MQ=255)
                                                           aGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTcg                                                                                   >  8:75469/1‑149 (MQ=255)
                                                              acacCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGa                                                                                <  4:52973/149‑1 (MQ=255)
                                                               cacCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGAc                                                                               <  6:317496/149‑1 (MQ=255)
                                                                acCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGAcc                                                                              >  6:192681/1‑149 (MQ=255)
                                                                  cccTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCa                                                                                                        <  7:187156/121‑1 (MQ=255)
                                                                  cccTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCa                                                                                                        >  8:187156/1‑121 (MQ=255)
                                                                    cTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGGTGTCATTGGGGAAACCGCCTGCCGCCCGCCCCCCGGCCTTGGCGCGGATCAGCTGCTCGGCCTGTGGGGTGGCGCCCCACg                                                                          >  8:260810/1‑149 (MQ=255)
                                                                                 gcCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTg                                                             <  2:273598/149‑1 (MQ=255)
                                                                                   cAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGc                                                           <  5:158842/149‑1 (MQ=255)
                                                                                     ggTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTc                                                                                    >  8:247839/1‑122 (MQ=255)
                                                                                     ggTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTc                                                                                    <  7:247839/122‑1 (MQ=255)
                                                                                               gCCGATGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGAt                                                 >  2:150972/1‑147 (MQ=255)
                                                                                                 cGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATcag                                              >  5:185719/1‑148 (MQ=255)
                                                                                                 cGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATcag                                              >  3:25799/1‑148 (MQ=255)
                                                                                                           gcgcgGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTgg                                   >  2:316219/1‑149 (MQ=255)
                                                                                                                 gAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGgcg                             >  2:280615/1‑149 (MQ=255)
                                                                                                                 gAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGgcg                             >  3:88507/1‑149 (MQ=255)
                                                                                                                   aCAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGcc                           >  3:94292/1‑149 (MQ=255)
                                                                                                                     aGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATcag                                              <  3:66675/128‑1 (MQ=255)
                                                                                                                     aGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATcag                                              >  4:66675/1‑128 (MQ=255)
                                                                                                                           tGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGc                      >  8:96818/1‑146 (MQ=255)
                                                                                                                           tGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGc                      <  7:96818/146‑1 (MQ=255)
                                                                                                                           tGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGCTTg                   >  4:12840/1‑149 (MQ=255)
                                                                                                                              cgcCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGCTTGCAg                <  4:88507/149‑1 (MQ=255)
                                                                                                                              cgcCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGCTTGCAg                <  5:192681/149‑1 (MQ=255)
                                                                                                                               gcCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTgg                                                            >  2:133479/1‑104 (MQ=255)
                                                                                                                                ccGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGCTTGCAGGt              <  3:12840/149‑1 (MQ=255)
                                                                                                                                          tCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATca                                               >  4:207680/1‑106 (MQ=255)
                                                                                                                                            aTTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCCCGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGCTTGCAGGTGCACACCCAGGt  >  5:107587/1‑149 (MQ=255)
                                                                                                                                            aTTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGCTTGCAGGTGCACACCCAGGt  <  4:25799/149‑1 (MQ=255)
                                                                                                                                            aTTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGCTTGCAGGTGCACACCCAGGt  <  6:185719/149‑1 (MQ=255)
                                                                                                                                                  gAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATcagcag                                           >  6:27690/1‑102 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GACTTCAGGTCGGTGGCAACCACGCCGCCGGCGGACTCGCCCAGCCGCAGGCCATCGCCAGAACACCCCTTGGGCGGCAGCGCCAGGTTGTCGTGGCCGCTGGCATCGCGCGGGAACAGTTGCTGGCGCCGGGCGTTGTCATTGGGGAAACCGCCTGCCGCCAGCACCACGGCCTTGGCGCGGATCAGCTGCTCGCCCTGTGGCGTCGCGACCCACGCCCCGCGCACCTGGCCACCCTCGATCAGCAGGCGTTTGGCCGGCGCCGACGCTTGCAGGTGCACACCCAGGT  >  NC_002947/2972909‑2973197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑