| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,021,986 | C→T | 9.6% | P842S (CCG→TCG) | PP_2638 → | cellulose synthase operon protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,021,986 | 0 | C | T | 9.6% | 143.3 / 4.2 | 52 | P842S (CCG→TCG) | PP_2638 | cellulose synthase operon protein C |
| Reads supporting (aligned to +/- strand): ref base C (21/26); new base T (3/2); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
TGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCGTTGGGTTGG > NC_002947/3021838‑3022120 | tGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGc < 2:141045/149‑1 (MQ=255) tGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCa < 3:288656/149‑1 (MQ=255) gcgAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAgg < 8:308759/147‑1 (MQ=255) gcgAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAgg > 7:308759/1‑147 (MQ=255) cgAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAg > 5:341540/1‑145 (MQ=255) cgAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAg < 6:341540/145‑1 (MQ=255) gCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGTATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACt > 8:39140/1‑149 (MQ=255) ccGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTc > 8:213656/1‑149 (MQ=255) cGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCTAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTc > 1:270440/1‑149 (MQ=255) gggaCTTCGGAACTGAGTAACCATTCGACCTTGTTGCATGGCGACACCCCTTGGC‑ACGAGGGGACCGCGTTTGGGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGACCCGTACGTTCAGGGAAGCTACACCCCCGACTTCgg < 2:3923/146‑1 (MQ=255) ttCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGGCTCGAACGacc < 3:84780/119‑3 (MQ=255) ttCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGGCTCGAACGacc > 4:84780/1‑117 (MQ=255) ttCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCt > 6:358738/1‑149 (MQ=255) cGGAACTGAGTACCCAGTCGACCTTGTTGCATTTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGc > 6:49291/1‑149 (MQ=255) cGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑ATGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGATCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCt < 1:82280/148‑1 (MQ=255) ggAACTGAGTACCCAGTCGACCTTGTTGCATTTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGc < 5:49291/148‑1 (MQ=255) tACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTa > 3:114083/1‑117 (MQ=255) tACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTa < 4:114083/117‑1 (MQ=255) cccAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGc < 2:270440/149‑1 (MQ=255) ccAGTCGACCTTGTTGTATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGcc < 7:39140/149‑1 (MQ=255) ccAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGGCTCGAACGacc > 3:255827/1‑102 (MQ=255) ccAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGGCTCGAACGacc < 4:255827/104‑3 (MQ=255) ccTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACtt > 6:139842/1‑118 (MQ=255) ccTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCa > 5:285648/1‑130 (MQ=255) ccTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCa < 6:285648/130‑1 (MQ=255) cTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTacac < 5:222257/108‑1 (MQ=255) cTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTacac > 6:222257/1‑108 (MQ=255) gttgCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGAc < 7:213656/148‑1 (MQ=255) gttgCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGAc < 3:282550/148‑1 (MQ=255) gACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAg > 2:156684/1‑84 (MQ=255) gACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAg < 1:156684/84‑1 (MQ=255) cccTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGc > 7:137941/1‑136 (MQ=255) cccTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGc < 8:137941/136‑1 (MQ=255) cccTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCcg > 1:188242/1‑149 (MQ=255) ccTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTTGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACtt < 4:309371/98‑1 (MQ=255) ccTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTTGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACtt > 3:309371/1‑98 (MQ=255) aCCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGc < 1:332401/105‑1 (MQ=255) aCCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGc > 2:332401/1‑105 (MQ=255) gTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCa > 6:104432/1‑147 (MQ=255) gTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCa < 5:104432/147‑1 (MQ=255) tttGCGAGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGAc < 2:188242/149‑1 (MQ=255) gagGGCATTGGGATGGATGGGGTG‑TCGTTCGAGCCGAACGTTCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACAGTGGCCGCGTGCACTGGGGGATCGCAGACTGCAAACGGCa < 3:167958/148‑1 (MQ=255) aTTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTg > 5:309748/1‑105 (MQ=255) aTTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTg < 6:309748/105‑1 (MQ=255) ttGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTg < 5:358738/149‑1 (MQ=255) gCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAg < 2:47826/61‑1 (MQ=255) cctgatCGTTCGGCTCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCgttgggttgg > 3:93315/6‑149 (MQ=255) g‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGa < 7:264949/113‑1 (MQ=255) g‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGa > 8:264949/1‑113 (MQ=255) g‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCgttgggtt > 8:137977/1‑143 (MQ=255) g‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCgttgggtt < 7:137977/143‑1 (MQ=255) aGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGtt > 3:31190/1‑124 (MQ=255) aGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGtt < 4:31190/124‑1 (MQ=255) | TGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGC‑AGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGG‑TCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCGTTGGGTTGG > NC_002947/3021838‑3022120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |