Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,037,048 A→G 8.3% I180T (ATC→ACC)  PP_2649 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,037,0480AG 8.3% 132.5 / 2.1 48I180T (ATC→ACC) PP_2649LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/23);  new base G (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGGGAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGAAC  >  NC_002947/3036901‑3037194
                                                                                                                                                   |                                                                                                                                                  
aGCGGGAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGAt                                                                                                                                                   <  3:88252/149‑1 (MQ=255)
 gCGGGAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGAt                                                                                                                                                   <  1:5673/148‑1 (MQ=255)
 gCGGGAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGAt                                                                                                                                                   >  2:5673/1‑148 (MQ=255)
    ggAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGGTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGAcc                                                                                                                                               <  5:341228/149‑1 (MQ=255)
     gAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCg                                                                                                                                              >  1:219165/1‑149 (MQ=255)
     gAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCg                                                                                                                                              >  4:166085/1‑149 (MQ=255)
        gcgcAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGc                                                                                                                                             >  7:69143/1‑147 (MQ=255)
          gcAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGGTCATCGCCaa                                                                                                                                          >  7:75273/1‑147 (MQ=255)
          gcAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAgt                                                                                                                                         <  1:188461/149‑1 (MQ=255)
            aGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGGTCATCGCCaacat                                                                                                                                       <  8:75273/149‑5 (MQ=255)
             gCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGtc                                                                                                                                      <  8:69143/149‑1 (MQ=255)
                                      cAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCg                                                                                                              >  7:25541/1‑148 (MQ=255)
                                      cAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCg                                                                                                              >  8:244133/1‑148 (MQ=255)
                                      cAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCg                                                                                                              >  5:130885/1‑148 (MQ=255)
                                         ttGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGaa                                                                                                            >  7:287330/1‑147 (MQ=255)
                                            cACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCt                                                                                                       >  6:44541/1‑149 (MQ=255)
                                             aCGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTg                                                                                                      >  3:324808/1‑149 (MQ=255)
                                              cgcgCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTgc                                                                                                     <  5:44541/149‑1 (MQ=255)
                                                 gcATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTgcggc                                                                                                  <  2:344305/149‑1 (MQ=255)
                                                     gggCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGTGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTAt                                                                                              >  5:103250/1‑149 (MQ=255)
                                                     gggCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTAt                                                                                              >  3:251210/1‑149 (MQ=255)
                                                      ggCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTAtt                                                                                             <  4:324808/149‑1 (MQ=255)
                                                                       gACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCgg                                                                             >  8:181117/1‑148 (MQ=255)
                                                                       gACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCgg                                                                             >  8:17910/1‑148 (MQ=255)
                                                                            tCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAgg                                                                       <  7:244133/149‑1 (MQ=255)
                                                                                 cGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGAt                                                                  <  8:287330/149‑1 (MQ=255)
                                                                                    ctctCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTc                                                               <  3:240585/149‑1 (MQ=255)
                                                                                             aGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGcc                                                      <  3:166085/149‑1 (MQ=255)
                                                                                              ggTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCg                                                     <  4:251210/149‑1 (MQ=255)
                                                                                                  ggCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGctct                                                 <  8:25541/149‑1 (MQ=255)
                                                                                                    cAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCtctc                                                >  2:148685/1‑148 (MQ=255)
                                                                                                        gCTCAGGCTTGGTGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGGCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCt                                           <  6:103250/149‑1 (MQ=255)
                                                                                                          tCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTgg                                         >  2:63047/1‑149 (MQ=255)
                                                                                                            aGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGt                                       <  6:130885/149‑1 (MQ=255)
                                                                                                                ttGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGctct                                                 <  5:122863/135‑1 (MQ=255)
                                                                                                                ttGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGctct                                                 >  6:122863/1‑135 (MQ=255)
                                                                                                                             gAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGcc                                                      >  6:52447/1‑117 (MQ=255)
                                                                                                                             gAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGcc                                                      <  5:52447/117‑1 (MQ=255)
                                                                                                                                      gtCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGc                                                                     >  7:140454/1‑93 (MQ=255)
                                                                                                                                      gtCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGc                                                                     <  8:140454/93‑1 (MQ=255)
                                                                                                                                      gtCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTc                  >  4:83671/1‑144 (MQ=255)
                                                                                                                                      gtCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTc                  <  3:83671/144‑1 (MQ=255)
                                                                                                                                         gacactGGCGGTCATCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGActggctg                       >  1:114171/6‑136 (MQ=255)
                                                                                                                                         gacactGGCGGTCATCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGActggctg                       <  2:114171/131‑1 (MQ=255)
                                                                                                                                                gCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGaa   <  1:63047/149‑1 (MQ=255)
                                                                                                                                                 cGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGAAc  <  1:148685/149‑1 (MQ=255)
                                                                                                                                                  gATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGaa   <  4:86967/147‑1 (MQ=255)
                                                                                                                                                  gATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGaa   >  3:86967/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGCGGGAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGAAC  >  NC_002947/3036901‑3037194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑