Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,037,050 G→C 8.9% V179V (GTC→GTG) ‡ PP_2649 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,037,0500GC 8.9% 138.4 / 4.6 45V179V (GTC→GTG) ‡PP_2649LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (20/21);  new base C (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GGAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGAAC  >  NC_002947/3036905‑3037194
                                                                                                                                                 |                                                                                                                                                
ggAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGGTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGAcc                                                                                                                                               <  5:341228/149‑1 (MQ=255)
 gAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCg                                                                                                                                              >  1:219165/1‑149 (MQ=255)
 gAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCg                                                                                                                                              >  4:166085/1‑149 (MQ=255)
    gcgcAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGc                                                                                                                                             >  7:69143/1‑147 (MQ=255)
      gcAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGGTCATCGCCaa                                                                                                                                          >  7:75273/1‑147 (MQ=255)
      gcAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAgt                                                                                                                                         <  1:188461/149‑1 (MQ=255)
        aGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGGTCATCGCCaacat                                                                                                                                       <  8:75273/149‑5 (MQ=255)
         gCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGtc                                                                                                                                      <  8:69143/149‑1 (MQ=255)
                                  cAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCg                                                                                                              >  5:130885/1‑148 (MQ=255)
                                  cAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCg                                                                                                              >  8:244133/1‑148 (MQ=255)
                                  cAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCg                                                                                                              >  7:25541/1‑148 (MQ=255)
                                     ttGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGaa                                                                                                            >  7:287330/1‑147 (MQ=255)
                                        cACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCt                                                                                                       >  6:44541/1‑149 (MQ=255)
                                         aCGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTg                                                                                                      >  3:324808/1‑149 (MQ=255)
                                          cgcgCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTgc                                                                                                     <  5:44541/149‑1 (MQ=255)
                                             gcATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTgcggc                                                                                                  <  2:344305/149‑1 (MQ=255)
                                                 gggCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGTGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTAt                                                                                              >  5:103250/1‑149 (MQ=255)
                                                 gggCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTAt                                                                                              >  3:251210/1‑149 (MQ=255)
                                                  ggCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTAtt                                                                                             <  4:324808/149‑1 (MQ=255)
                                                                   gACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCgg                                                                             >  8:181117/1‑148 (MQ=255)
                                                                   gACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCgg                                                                             >  8:17910/1‑148 (MQ=255)
                                                                        tCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAgg                                                                       <  7:244133/149‑1 (MQ=255)
                                                                             cGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGAt                                                                  <  8:287330/149‑1 (MQ=255)
                                                                                ctctCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTc                                                               <  3:240585/149‑1 (MQ=255)
                                                                                         aGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGcc                                                      <  3:166085/149‑1 (MQ=255)
                                                                                          ggTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCg                                                     <  4:251210/149‑1 (MQ=255)
                                                                                              ggCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGctct                                                 <  8:25541/149‑1 (MQ=255)
                                                                                                cAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCtctc                                                >  2:148685/1‑148 (MQ=255)
                                                                                                    gCTCAGGCTTGGTGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGGCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCt                                           <  6:103250/149‑1 (MQ=255)
                                                                                                      tCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTgg                                         >  2:63047/1‑149 (MQ=255)
                                                                                                        aGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGt                                       <  6:130885/149‑1 (MQ=255)
                                                                                                            ttGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGctct                                                 >  6:122863/1‑135 (MQ=255)
                                                                                                            ttGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGctct                                                 <  5:122863/135‑1 (MQ=255)
                                                                                                                         gAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGcc                                                      >  6:52447/1‑117 (MQ=255)
                                                                                                                         gAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGcc                                                      <  5:52447/117‑1 (MQ=255)
                                                                                                                                  gtCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGc                                                                     >  7:140454/1‑93 (MQ=255)
                                                                                                                                  gtCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGc                                                                     <  8:140454/93‑1 (MQ=255)
                                                                                                                                  gtCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTc                  >  4:83671/1‑144 (MQ=255)
                                                                                                                                  gtCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTc                  <  3:83671/144‑1 (MQ=255)
                                                                                                                                     gacactGGCGGTCATCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGActggctg                       >  1:114171/6‑136 (MQ=255)
                                                                                                                                     gacactGGCGGTCATCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGActggctg                       <  2:114171/131‑1 (MQ=255)
                                                                                                                                            gCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGaa   <  1:63047/149‑1 (MQ=255)
                                                                                                                                             cGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGAAc  <  1:148685/149‑1 (MQ=255)
                                                                                                                                              gATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGaa   <  4:86967/147‑1 (MQ=255)
                                                                                                                                              gATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGaa   >  3:86967/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGAAGCGCAGCCCGGCATCGTGAAATTCGCGCTCCAGTTGCACGCGCATGGGCATGTTGGCCCGGTAGACGATCCAGCGGCTCTCGGCCAGGTCGGCAAGGCTCAGGCTTGGCGCATGCTTGAGCGGGTGGTCGATGTTGGCGATGACCGCCAGTGTCTCATCCTGGACAAAGGTGCTGGCGAACAGCTGCGGCGTATTGCTGACACTGGTGCGGCACAGGGCGATGTCCAGGCGGCCGCTCTCGATCTGGGTCAGCAGCGACTGGCTGGTGTCTTCGATGATTTCGAAC  >  NC_002947/3036905‑3037194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑