Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,065,515	T→G	6.1%	V152G (GTG→GGG) ‡	PP_2677 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,065,515	0	T	G	6.1%	197.3 / 4.2	66	V152G (GTG→GGG) ‡	PP_2677	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/32);  new base G (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

AGCGCGTGTTGCCCTGGTTGTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAAC  >  NC_002947/3065371‑3065663                                                                                                                                                 |                                                                                                                                                     aGCGCGTGTTGCCCTGGTTGTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCgtggtg                                                                                                                                                  <  7:132223/149‑1 (MQ=255)        gtTGCCCTGGTTGTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCAcc                                                                                                                                            >  3:314698/1‑148 (MQ=255)           gctcTGGTTGTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTCTACTGACGCGCGATCGCCAGAGGCACGTCGGATCGACCCTGATCCATGCGGCCGCCGGCGGTAGTACCGCACCCAGCGTGGTGCGCACCCAGc                                                                                                                                        >  2:167456/4‑149 (MQ=255)           gCCCTGGTTGTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAg                                                                                                                                         <  8:245809/148‑1 (MQ=255)                    gTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTgg                                                                                                                                <  1:167456/148‑1 (MQ=255)                    gTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTgg                                                                                                                                <  4:28637/148‑1 (MQ=255)                     tCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGgga                                                                                                                              <  5:187046/149‑1 (MQ=255)                       gATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCTCCCAGccc                                                                                                                                      <  3:156222/139‑1 (MQ=255)                       gATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCTCCCAGccc                                                                                                                                      >  4:156222/1‑139 (MQ=255)                       gATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGggag                                                                                                                             >  4:54943/1‑148 (MQ=255)                        atccgcatccgcATCGAACAGGCCACACGGCTGATCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGtgagga                                                                                                                           <  6:167915/149‑1 (MQ=255)                        atccgcatccgcATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGggag                                                                                                                             >  1:275281/1‑147 (MQ=255)                         tccgcatccgcaTCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGgaggag                                                                                                                          >  8:164743/1‑149 (MQ=255)                           cgcatccgcatcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGc                                                                                                                         <  4:314698/148‑1 (MQ=255)                             catccgcatcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCGGGGAGGAGCAcc                                                                                                                      <  1:298018/149‑1 (MQ=255)                                ccgcatcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCg                                                                                                                   >  8:186835/1‑149 (MQ=255)                                  gcatcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTg                                                                                                                                 >  2:8650/1‑133 (MQ=255)                                  gcatcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTg                                                                                                                                 <  1:8650/133‑1 (MQ=255)                                  gcatcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCCGGGAGGAGCACCTCggg                                                                                                                 >  7:8387/1‑149 (MQ=255)                                   catcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCa                                                                                                                                              <  2:266397/119‑1 (MQ=255)                                   catcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCa                                                                                                                                              >  1:266397/1‑119 (MQ=255)                                     tcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCCGGGAGGAGCACCTCGGGGAg                                                                                                              <  8:8387/149‑1 (MQ=255)                                          cAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCt                                                                                                         <  7:186835/149‑1 (MQ=255)                                           aGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTg                                                                                                        <  2:275281/149‑1 (MQ=255)                                           aGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTg                                                                                                        <  7:164743/149‑1 (MQ=255)                                             gCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCg                                                                                                                                     >  2:31979/1‑118 (MQ=255)                                             gCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCg                                                                                                                                     <  1:31979/118‑1 (MQ=255)                                             gCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTggg                                                                                                      >  3:149616/1‑149 (MQ=255)                                               cacaCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCACGCTGGGTgcggt                                                                                                                                              <  2:302079/107‑3 (MQ=255)                                               cacaCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCACGCTGGGTgcggt                                                                                                                                              <  2:307097/107‑3 (MQ=255)                                               cacaCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCACGCTGGGTgcggt                                                                                                                                              >  1:307097/1‑105 (MQ=255)                                               cacaCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCACGCTGGGTgcggt                                                                                                                                              >  1:302079/1‑105 (MQ=255)                                                acacCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTg                                                                                                           >  3:259286/1‑141 (MQ=255)                                                acacCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTg                                                                                                           <  4:259286/141‑1 (MQ=255)                                                             ccgcTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGt                                                                                      <  3:54943/149‑1 (MQ=255)                                                             ccgcTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGt                                                                                      <  5:140349/149‑1 (MQ=255)                                                                      tGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAcag                                                                              >  1:311380/1‑148 (MQ=255)                                                                       gACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAcag                                                                              >  2:127248/1‑147 (MQ=255)                                                                          gcgcgATGGCCTGTGGCACGTCGGTTCGACCCTGCTCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGt                                                                                      >  2:92890/1‑136 (MQ=255)                                                                          gcgcgATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGt                                                                                      <  1:92890/136‑1 (MQ=255)                                                                              gATGGCCTGTGGCACGGCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcct                                                                     <  7:324970/149‑1 (MQ=255)                                                                                   ccTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcc                                                                >  5:48422/1‑149 (MQ=255)                                                                                    cTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcct                                                               >  3:17881/1‑149 (MQ=255)                                                                                    cTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcct                                                               >  6:134068/1‑149 (MQ=255)                                                                                         gCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCGGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAg                                                           <  5:134068/148‑1 (MQ=255)                                                                                          cACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGt                                                         <  1:127248/149‑1 (MQ=255)                                                                                           aCGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAcag                                                                              >  5:161467/1‑127 (MQ=255)                                                                                           aCGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAcag                                                                              <  6:161467/127‑1 (MQ=255)                                                                                           aCGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTg                                                        >  5:253141/1‑149 (MQ=255)                                                                                            cGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTgg                                                       <  2:311380/149‑1 (MQ=255)                                                                                                ggTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCCGGTGGCCCa                                                   >  2:344408/1‑149 (MQ=255)                                                                                                  ttCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCAcc                                                 <  4:17881/149‑1 (MQ=255)                                                                                                        ccTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATg                                            >  2:307345/1‑148 (MQ=255)                                                                                                        ccTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATg                                            >  7:201632/1‑148 (MQ=255)                                                                                                         cTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGa                                          >  7:248184/1‑149 (MQ=255)                                                                                                          tGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAg                                                           <  7:329064/131‑1 (MQ=255)                                                                                                          tGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAg                                                           >  8:329064/1‑131 (MQ=255)                                                                                                           gATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGAGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGAt                                         >  5:250916/1‑148 (MQ=255)                                                                                                               gATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACgg                                    >  6:297147/1‑149 (MQ=255)                                                                                                                               gTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTgg                                                                                    >  3:142292/1‑85 (MQ=255)                                                                                                                                ttGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTc                   <  1:307345/149‑1 (MQ=255)                                                                                                                                   tACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCt                 <  8:248184/148‑1 (MQ=255)                                                                                                                                     ccGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGa               <  6:48422/148‑1 (MQ=255)                                                                                                                                     ccGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGAGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGa               <  6:250916/148‑1 (MQ=255)                                                                                                                                        cACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttc           <  8:201632/149‑1 (MQ=255)                                                                                                                                           ccAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttcttt        <  1:136946/149‑1 (MQ=255)                                                                                                                                             aGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttctttct      >  4:112781/1‑149 (MQ=255)                                                                                                                                                 tggtgCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCTTGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAAc  >  2:181805/1‑149 (MQ=255)                                                                                                                                                 tggtgCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAAc  >  8:192015/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                     AGCGCGTGTTGCCCTGGTTGTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAAC  >  NC_002947/3065371‑3065663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑