Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,136,992 T→A 7.8% V65D (GTT→GAT) ‡ PP_2753 → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,136,9920TA 7.8% 145.1 / 3.3 51V65D (GTT→GAT) ‡PP_2753ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base A (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

ATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGA  >  NC_002947/3136856‑3137136
                                                                                                                                         |                                                                                                                                                 
aTCGAAGTGATCTACGACGGCACGATCCTTGCCGTTGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGAACCAGGCCggaaa                                                                                                                                       <  3:86173/149‑5 (MQ=255)
aTCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCg                                                                                                                                       >  5:189516/1‑149 (MQ=255)
   gAAGTTATCTACGACGGAACTATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCggg                                                                                                                                     <  6:248168/148‑1 (MQ=255)
    aaGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGgcg                                                                                                                                   >  4:319179/1‑149 (MQ=255)
     aGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGgcgc                                                                                                                                  <  2:111172/149‑1 (MQ=255)
              cgacgGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGt                                                                                                                              <  8:77990/144‑1 (MQ=255)
              cgacgGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGt                                                                                                                              >  7:77990/1‑144 (MQ=255)
               gacgGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCg                                                                                                                        >  6:41167/1‑149 (MQ=255)
                actgCACGATCCTTGCCGTGGCCGGTGTATCCCTGCTGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGTCCGACCGGGCGCATGTCAGTCGt                                                                                                                       >  2:101729/4‑149 (MQ=255)
                  ggCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATTTCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑gg                                                                                                                    <  3:319179/149‑1 (MQ=255)
                           cTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCg                                                                                                           <  6:189516/149‑1 (MQ=255)
                            ttGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCACAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGc                                                                                                          >  3:275300/1‑149 (MQ=255)
                                 gTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGGCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTc                                                                                                       >  1:252904/1‑148 (MQ=255)
                                 gTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTc                                                                                                       >  7:237153/1‑147 (MQ=255)
                                 gTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCa                                                                                                     <  3:320154/149‑1 (MQ=255)
                                     ccGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAgggg                                                                                                 <  1:101729/149‑1 (MQ=255)
                                               ccTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACAccg                                                                                       >  1:242316/1‑149 (MQ=255)
                                                     ggTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGgtg                                                                                 <  2:252904/149‑1 (MQ=255)
                                                     ggTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGgtg                                                                                 <  8:237153/149‑1 (MQ=255)
                                                           gCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCgg                                                                           >  2:8909/1‑149 (MQ=255)
                                                             aGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGc                                                                          >  5:27788/1‑148 (MQ=255)
                                                                 cGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAAcc                                                                     >  7:281601/1‑149 (MQ=255)
                                                                  gATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGgc                                                                                                                                    <  7:162032/86‑1 (MQ=255)
                                                                  gATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGgc                                                                                                                                    >  8:162032/1‑86 (MQ=255)
                                                                      tcgtcgCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACctgctg                                                                <  5:41167/149‑1 (MQ=255)
                                                                         tcgCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCa                                                             >  5:113735/1‑149 (MQ=255)
                                                                              tt‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGc                                                          >  5:54420/1‑147 (MQ=255)
                                                                                 gCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCgg                                                                           >  1:156752/1‑128 (MQ=255)
                                                                                 gCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCgg                                                                           <  2:156752/128‑1 (MQ=255)
                                                                                  cTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAgg                                                      <  6:113735/148‑1 (MQ=255)
                                                                                   tAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAg                                                                                              <  1:280961/107‑1 (MQ=255)
                                                                                   tAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAg                                                                                              >  2:280961/1‑107 (MQ=255)
                                                                                              ggTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCAtgt                                         <  7:189964/149‑1 (MQ=255)
                                                                                                  cTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGtt                                      <  2:95168/148‑1 (MQ=255)
                                                                                                  cTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTg                                     <  2:242316/149‑1 (MQ=255)
                                                                                                   tGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTg                                     >  5:326641/1‑148 (MQ=255)
                                                                                                      cAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACTCCAGGGTATCGTCCATGTGTTGGAAg                                 <  1:8909/149‑1 (MQ=255)
                                                                                                        agagCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCAGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCACCCTGCTGGCACGCCAGGGTATCGGCCATGTGTTGGAAgta                               >  8:142111/1‑147 (MQ=255)
                                                                                                                acGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATATCAGTCGT‑GGCCATATACGCTTGCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCAtgt                       >  1:72663/1‑149 (MQ=255)
                                                                                                                  gcacAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATgtgt                     <  4:275300/146‑1 (MQ=255)
                                                                                                                    tCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTAGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGtt                    >  1:88080/1‑149 (MQ=255)
                                                                                                                      aaGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTtc                   <  6:326641/147‑1 (MQ=255)
                                                                                                                           tATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTcc                >  7:206352/1‑145 (MQ=255)
                                                                                                                           tATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTcc                <  8:206352/145‑1 (MQ=255)
                                                                                                                               gtcGGCCTGAACCAGGCCGACCGGGCGCATGTCAGTCGT‑GTCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCt           <  6:345669/144‑1 (MQ=255)
                                                                                                                               gtcGGCCTGAACCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGag        >  5:345669/3‑148 (MQ=255)
                                                                                                                                tcGGCCTGAACCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGAc        >  4:283451/2‑148 (MQ=255)
                                                                                                                                 cGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCGGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTTTCGTACATGTGtt                                      <  7:92081/117‑1 (MQ=255)
                                                                                                                                 cGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATACGCTTCCATGGGCAGGACACCGCCGGTCTCGCGGCCAACCAGCTAGCACGCCAGGGTATCGTCCATGTGtt                                      >  8:92081/1‑117 (MQ=255)
                                                                                                                                    ccTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCg   <  6:54420/149‑1 (MQ=255)
                                                                                                                                     cTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGa  <  2:72663/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                 
ATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTT‑GCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGT‑GGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGA  >  NC_002947/3136856‑3137136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑