Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,141,000 A→T 6.5% E100D (GAA→GAT) ‡ PP_2756 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,141,0000AT 6.5% 178.4 / 2.8 62E100D (GAA→GAT) ‡PP_2756hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/31);  new base T (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

CGACGCGTTGCTGGCACGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTGGCGG  >  NC_002947/3140853‑3141132
                                                                                                                                                    |                                                                                                                                    
cgACGCGTTGCTGGCACGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAAc                                                                                                                                     <  4:96693/149‑1 (MQ=255)
      gTTGCTGGCACGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCAc                                                                                                                               >  3:247439/1‑149 (MQ=255)
          cTGGCACGCGCCGATGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGTGCCGGTTCGGCGCTGcc                                                                                                                           <  2:246021/149‑1 (MQ=255)
             gCACGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCa                                                                                                                         <  4:247439/148‑1 (MQ=255)
               aCGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACt                                                                                                                       >  1:246374/1‑148 (MQ=255)
               aCGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACt                                                                                                                       >  4:157372/1‑148 (MQ=255)
                 gcgcCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCaa                                                                                                                    >  5:34067/1‑149 (MQ=255)
                     cGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGc                                                                                                                <  6:67042/149‑1 (MQ=255)
                     cGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGc                                                                                                                <  3:157372/149‑1 (MQ=255)
                       aCGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGa                                                                                                              <  1:222765/149‑1 (MQ=255)
                         gCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGTGCCGGTTCGGCGCTGccc                                                                                                                          >  5:100362/1‑135 (MQ=255)
                         gCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGTGCCGGTTCGGCGCTGccc                                                                                                                          <  6:100362/135‑1 (MQ=255)
                         gCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCCAACCCGCACAGCCCACTCAACCGCCAc                                                                                                             >  8:311375/1‑148 (MQ=255)
                           cTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGAGGGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTg                                                                                                           >  1:243192/1‑149 (MQ=255)
                           cTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGc                                                                                                          >  6:188151/1‑149 (MQ=255)
                             cGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTc                                                                                                        >  7:111419/1‑149 (MQ=255)
                                       cgccATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACt                                                                                              <  2:243192/149‑1 (MQ=255)
                                       cgccATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACt                                                                                              <  6:34067/149‑1 (MQ=255)
                                         ccATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTAc                                                                                            >  8:163402/1‑149 (MQ=255)
                                                  ttCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACg                                                                                     <  8:111419/147‑1 (MQ=255)
                                                    cGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGgcg                                                                                  <  6:105733/148‑1 (MQ=255)
                                                      tttGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTACCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGcgccg                                                                               >  7:22180/1‑149 (MQ=255)
                                                            cgccgcCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTgg                                                                         >  4:287842/1‑149 (MQ=255)
                                                               cgccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTgg                                                                         >  8:94895/1‑146 (MQ=255)
                                                                 ccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTAc                                                                                            <  5:38908/125‑1 (MQ=255)
                                                                 ccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTAc                                                                                            >  6:38908/1‑125 (MQ=255)
                                                                 ccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGAt                                                                    <  1:279429/149‑1 (MQ=255)
                                                                 ccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGAt                                                                    <  7:311375/149‑1 (MQ=255)
                                                                    a‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCTCCGCCTTGGCAGATGAg                                                                 <  5:188151/149‑1 (MQ=255)
                                                                                  ggcggcGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCa                                                      <  6:107794/147‑1 (MQ=255)
                                                                                   gCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAg                                                     <  2:246374/147‑1 (MQ=255)
                                                                                   gCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAg                                                     <  7:163402/147‑1 (MQ=255)
                                                                                     cgcgCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCAt                                                 <  3:287842/149‑1 (MQ=255)
                                                                                       cgcAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTACCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGa                                               <  8:22180/149‑1 (MQ=255)
                                                                                         cAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGt                                             <  7:94895/149‑1 (MQ=255)
                                                                                                 tttCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGc                                                   >  3:236412/1‑135 (MQ=255)
                                                                                                 tttCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGc                                                   <  4:236412/135‑1 (MQ=255)
                                                                                                 tttCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCt                                     >  1:210828/1‑149 (MQ=255)
                                                                                                      aaCTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCa                                                                       <  6:248218/110‑1 (MQ=255)
                                                                                                      aaCTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCa                                                                       >  5:248218/1‑110 (MQ=255)
                                                                                                      aaCTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCaaa                                                             <  6:364685/120‑1 (MQ=255)
                                                                                                      aaCTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCaaa                                                             >  5:364685/1‑120 (MQ=255)
                                                                                                         tGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGcgccgc                                                                              >  5:226234/1‑100 (MQ=255)
                                                                                                         tGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGcgccgc                                                                              <  6:226234/100‑1 (MQ=255)
                                                                                                           gTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCt                            >  7:111131/1‑148 (MQ=255)
                                                                                                                  gtagtCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTc                    <  7:179683/149‑1 (MQ=255)
                                                                                                                     gtCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTgg                 <  8:111131/149‑1 (MQ=255)
                                                                                                                      tCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGCATCCTGACGTTGCTCACCGTTCTGGt                >  5:107471/1‑149 (MQ=255)
                                                                                                                        tGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGc              >  7:213980/1‑149 (MQ=255)
                                                                                                                          ccGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGtt                     <  6:184483/140‑1 (MQ=255)
                                                                                                                          ccGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGtt                     >  5:184483/1‑140 (MQ=255)
                                                                                                                          ccGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTc                    >  4:160950/1‑141 (MQ=255)
                                                                                                                          ccGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTc                    <  3:160950/141‑1 (MQ=255)
                                                                                                                           cgcgTCCACAGCCGGGCAGCGCCAAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGa           <  8:195205/149‑1 (MQ=255)
                                                                                                                                ccACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGt                                             >  7:255028/1‑110 (MQ=255)
                                                                                                                                ccACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGt                                             <  8:255028/110‑1 (MQ=255)
                                                                                                                                 cacaGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGCGTCGAATCCTGACGTTGCTCACCGGTCTGGTGCTGACATTgg     >  5:215556/1‑149 (MQ=255)
                                                                                                                                 cacaGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTgg     >  7:153740/1‑149 (MQ=255)
                                                                                                                                 cacaGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTg      <  8:153740/148‑1 (MQ=255)
                                                                                                                                    gagtgggCAGTGCCGGTTCGGCGCTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTggcgg  >  7:55085/5‑149 (MQ=255)
                                                                                                                                     gCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTg                  >  8:165588/1‑132 (MQ=255)
                                                                                                                                     gCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTg                  <  7:165588/132‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                    
CGACGCGTTGCTGGCACGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTGGCGG  >  NC_002947/3140853‑3141132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑