Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 308,739 A→G 8.6% intergenic (+1754/‑115) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947308,7390AG 8.6% 223.9 / 8.6 81intergenic (+1754/‑115)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (38/36);  new base G (3/4);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCTCC  >  NC_002947/308600‑308886
                                                                                                                                           |                                                                                                                                                   
aGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCt                                                                                                                                            >  6:11153/1‑149 (MQ=255)
 gCGGCCTACGACGAGACTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCtt                                                                                                                                           >  3:96291/1‑149 (MQ=255)
      cTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtg                                                                                                                                      >  7:277217/1‑149 (MQ=255)
      cTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtg                                                                                                                                      >  2:120930/1‑149 (MQ=255)
      cTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTACAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCAGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtg                                                                                                                                      >  6:196315/1‑149 (MQ=255)
          gacgaGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                                   >  1:164629/1‑148 (MQ=255)
          gacgaGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                                   <  2:164629/148‑1 (MQ=255)
             gaGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGt                                                                                                                                 >  4:294629/1‑147 (MQ=255)
               tgCTGCCAAAGGTCTGGCCAAGGTGTTCATCGAAAACTTGAAGAAGTTCGAAGTTTCCGACGGCATCAAGGGCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACTAGAAAGCCGCACCCAGGGGCGGCTTTTTTGTGGGTTAgg                                                                                                                             <  3:212743/148‑1 (MQ=255)
               ggCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAgg                                                                                                                             <  8:164256/149‑1 (MQ=255)
                gCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAggg                                                                                                                            <  1:132938/149‑1 (MQ=255)
                 cTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGc                                                                                                                           <  5:11153/149‑1 (MQ=255)
                 cTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGc                                                                                                                           <  4:35757/149‑1 (MQ=255)
                          gTCTGGCCAAGCCGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCcgc                                                                                                                   >  3:292303/1‑148 (MQ=255)
                               gCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAggg                                                                                                             >  2:170045/1‑149 (MQ=255)
                               gCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCACCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAg                                                                                                               <  5:3562/147‑1 (MQ=255)
                               gCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCACCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAg                                                                                                               >  6:3562/1‑147 (MQ=255)
                                  aaGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCTGCGAAGGGGcc                                                                                                          >  7:160199/1‑149 (MQ=255)
                                    gCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCg                                                                                                        >  3:125265/1‑149 (MQ=255)
                                        ttCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCAt                                                                                                     <  7:108663/148‑1 (MQ=255)
                                        ttCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATa                                                                                                    <  1:120930/149‑1 (MQ=255)
                                          cATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAg                                                                                                   <  1:170045/148‑1 (MQ=255)
                                               aaaaCTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGtt                                                                                                                                >  3:305871/1‑114 (MQ=255)
                                               aaaaCTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGtt                                                                                                                                <  4:305871/114‑1 (MQ=255)
                                                  aCTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGaaa                                                                                          <  8:277217/149‑1 (MQ=255)
                                                    ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCtt                                                                                                                         >  4:97554/1‑116 (MQ=255)
                                                    ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCtt                                                                                                                         <  3:97554/116‑1 (MQ=255)
                                                    ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGccc                                                                                                         <  8:258267/132‑1 (MQ=255)
                                                    ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGccc                                                                                                         >  7:258267/1‑132 (MQ=255)
                                                    ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGc                                                                                                           <  7:241412/130‑1 (MQ=255)
                                                    ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGc                                                                                                           >  8:241412/1‑130 (MQ=255)
                                                    ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                                        >  8:186133/1‑149 (MQ=255)
                                                     tCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTc                                                                                       >  6:340717/1‑149 (MQ=255)
                                                      cAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCt                                                                                      >  5:164770/1‑149 (MQ=255)
                                                       aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCt                                                                                      <  7:186133/148‑1 (MQ=255)
                                                       aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTg                                                                                     <  6:135119/149‑1 (MQ=255)
                                                        agaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTgg                                                                                    <  3:294629/149‑1 (MQ=255)
                                                            gTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGtttt                                                                                >  2:89499/1‑149 (MQ=255)
                                                             ttCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttttt                                                                               <  4:125265/149‑1 (MQ=255)
                                                                gAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGt                                                                                   >  6:310943/1‑142 (MQ=255)
                                                                gAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGt                                                                                   <  5:310943/142‑1 (MQ=255)
                                                                 aaGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGGGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGcc                                                                                                          <  8:284489/118‑1 (MQ=255)
                                                                 aaGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGGGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGcc                                                                                                          >  7:284489/1‑118 (MQ=255)
                                                                    tttCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGttt                                                                         >  3:330289/1‑148 (MQ=255)
                                                                       ccGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAATCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgt                                                                                                                                     >  8:5836/1‑85 (MQ=255)
                                                                          aCGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttttt                                                                               >  4:320524/1‑136 (MQ=255)
                                                                          aCGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttttt                                                                               <  3:320524/136‑1 (MQ=255)
                                                                           cGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGTGCTTGCCCTCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCa                                                                 <  5:110993/149‑1 (MQ=255)
                                                                            gCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCTGGGGGCGGCtttctc                                                                                                                                       <  8:145032/78‑4 (MQ=38)
                                                                            gCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCTGGGGGCGGCtttctc                                                                                                                                       >  7:145032/1‑75 (MQ=38)
                                                                              cATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGATTTTCTGTTTCACTGTCAGCa                                                              >  6:335580/1‑149 (MQ=255)
                                                                               aTCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCAt                                                             <  5:196315/149‑1 (MQ=255)
                                                                               aTCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAAAAAGCCGCCCCTGGGGGCGGCtttctc                                                                                                                                       >  8:154134/1‑72 (MQ=37)
                                                                               aTCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAAAAAGCCGCCCCTGGGGGCGGCtttctc                                                                                                                                       <  7:154134/75‑4 (MQ=37)
                                                                                  aaGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCa                                                          >  2:54918/1‑149 (MQ=255)
                                                                                  aaGGCCGCTGGCCCGCAGCTGTAAGCAGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCa                                                          >  1:185067/1‑149 (MQ=255)
                                                                                    ggCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAtt                                                        >  7:28122/1‑149 (MQ=255)
                                                                                     gCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTg                                                       <  4:292303/149‑1 (MQ=255)
                                                                                      ccGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGc                                                      <  5:340717/149‑1 (MQ=255)
                                                                                         cTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGaa                                                   <  1:89499/149‑1 (MQ=255)
                                                                                                         atccGAGCTCAGCCGCAACTAGAAATTCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATATTCACAGAAAGTCTGGTTTTTCTGTTTCa                                                                       <  7:52599/111‑1 (MQ=255)
                                                                                                         aGCCGAGCTCAGCCGCAACGAGAAAGTCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCa                                                                       >  8:52599/1‑113 (MQ=255)
                                                                                                          gCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccagc                                      >  4:106062/1‑145 (MQ=255)
                                                                                                          gCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGGTAGGGCTTGCCCGCGAAGGGGCCCGCAGAGGCAGCGAAAGTCTGGTTTTTCTGTTTCa                                                                       >  5:126982/1‑112 (MQ=255)
                                                                                                                 tCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAtt                                                        <  8:318530/120‑1 (MQ=255)
                                                                                                                 tCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAtt                                                        >  7:318530/1‑120 (MQ=255)
                                                                                                                 tCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCg                            >  3:167607/1‑148 (MQ=255)
                                                                                                                          aCGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCa                  <  6:164770/149‑1 (MQ=255)
                                                                                                                            gagaAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAtt                                                        <  8:23245/109‑1 (MQ=255)
                                                                                                                            gagaAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAtt                                                        >  7:23245/1‑109 (MQ=255)
                                                                                                                            aaaaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACt                                                                     >  7:183394/4‑96 (MQ=255)
                                                                                                                            aaaaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACt                                                                     <  8:183394/93‑1 (MQ=255)
                                                                                                                              aaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGaa                                                                                                                <  2:141729/50‑1 (MQ=25)
                                                                                                                               aaaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCa             <  4:167607/149‑1 (MQ=255)
                                                                                                                               aaaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCa             >  8:223366/1‑149 (MQ=255)
                                                                                                                                   ccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGAc                              <  2:313079/128‑1 (MQ=255)
                                                                                                                                   ccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGAc                              >  1:313079/1‑128 (MQ=255)
                                                                                                                                    cgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGcc        <  1:54918/149‑1 (MQ=255)
                                                                                                                                        cccAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGaa                                                   >  1:323505/1‑102 (MQ=255)
                                                                                                                                        cccAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGaa                                                   <  2:323505/102‑1 (MQ=255)
                                                                                                                                          cAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCTcc  <  7:223366/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
AGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCTCC  >  NC_002947/308600‑308886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑