Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,141,006 A→G 7.3% A102A (GCA→GCG)  PP_2756 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,141,0060AG 7.3% 187.5 / 4.5 68A102A (GCA→GCG) PP_2756hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (31/32);  new base G (2/3);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

GTTGCTGGCACGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTGGCGGGCGGCCTGGCACACGCCGAG  >  NC_002947/3140859‑3141152
                                                                                                                                                    |                                                                                                                                                  
gTTGCTGGCACGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCAc                                                                                                                                                   >  3:247439/1‑149 (MQ=255)
    cTGGCACGCGCCGATGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGTGCCGGTTCGGCGCTGcc                                                                                                                                               <  2:246021/149‑1 (MQ=255)
       gCACGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCa                                                                                                                                             <  4:247439/148‑1 (MQ=255)
         aCGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACt                                                                                                                                           >  1:246374/1‑148 (MQ=255)
         aCGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACt                                                                                                                                           >  4:157372/1‑148 (MQ=255)
           gcgcCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCaa                                                                                                                                        >  5:34067/1‑149 (MQ=255)
               cGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGc                                                                                                                                    <  3:157372/149‑1 (MQ=255)
               cGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGc                                                                                                                                    <  6:67042/149‑1 (MQ=255)
                 aCGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGa                                                                                                                                  <  1:222765/149‑1 (MQ=255)
                   gCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGTGCCGGTTCGGCGCTGccc                                                                                                                                              >  5:100362/1‑135 (MQ=255)
                   gCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGTGCCGGTTCGGCGCTGccc                                                                                                                                              <  6:100362/135‑1 (MQ=255)
                   gCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCCAACCCGCACAGCCCACTCAACCGCCAc                                                                                                                                 >  8:311375/1‑148 (MQ=255)
                     cTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGAGGGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTg                                                                                                                               >  1:243192/1‑149 (MQ=255)
                     cTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGc                                                                                                                              >  6:188151/1‑149 (MQ=255)
                       cGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTc                                                                                                                            >  7:111419/1‑149 (MQ=255)
                                 cgccATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACt                                                                                                                  <  6:34067/149‑1 (MQ=255)
                                 cgccATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACt                                                                                                                  <  2:243192/149‑1 (MQ=255)
                                   ccATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTAc                                                                                                                >  8:163402/1‑149 (MQ=255)
                                            ttCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACg                                                                                                         <  8:111419/147‑1 (MQ=255)
                                              cGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGgcg                                                                                                      <  6:105733/148‑1 (MQ=255)
                                                tttGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTACCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGcgccg                                                                                                   >  7:22180/1‑149 (MQ=255)
                                                      cgccgcCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTgg                                                                                             >  4:287842/1‑149 (MQ=255)
                                                         cgccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTgg                                                                                             >  8:94895/1‑146 (MQ=255)
                                                           ccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTAc                                                                                                                <  5:38908/125‑1 (MQ=255)
                                                           ccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTAc                                                                                                                >  6:38908/1‑125 (MQ=255)
                                                           ccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGAt                                                                                        <  1:279429/149‑1 (MQ=255)
                                                           ccgA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGAt                                                                                        <  7:311375/149‑1 (MQ=255)
                                                              a‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCTCCGCCTTGGCAGATGAg                                                                                     <  5:188151/149‑1 (MQ=255)
                                                                            ggcggcGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCa                                                                          <  6:107794/147‑1 (MQ=255)
                                                                             gCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAg                                                                         <  7:163402/147‑1 (MQ=255)
                                                                             gCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAg                                                                         <  2:246374/147‑1 (MQ=255)
                                                                               cgcgCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCAt                                                                     <  3:287842/149‑1 (MQ=255)
                                                                                 cgcAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTACCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGa                                                                   <  8:22180/149‑1 (MQ=255)
                                                                                   cAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGt                                                                 <  7:94895/149‑1 (MQ=255)
                                                                                           tttCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGc                                                                       <  4:236412/135‑1 (MQ=255)
                                                                                           tttCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGc                                                                       >  3:236412/1‑135 (MQ=255)
                                                                                           tttCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCt                                                         >  1:210828/1‑149 (MQ=255)
                                                                                                aaCTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCa                                                                                           <  6:248218/110‑1 (MQ=255)
                                                                                                aaCTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCa                                                                                           >  5:248218/1‑110 (MQ=255)
                                                                                                aaCTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCaaa                                                                                 <  6:364685/120‑1 (MQ=255)
                                                                                                aaCTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCaaa                                                                                 >  5:364685/1‑120 (MQ=255)
                                                                                                   tGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGcgccgc                                                                                                  <  6:226234/100‑1 (MQ=255)
                                                                                                   tGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGcgccgc                                                                                                  >  5:226234/1‑100 (MQ=255)
                                                                                                     gTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCt                                                >  7:111131/1‑148 (MQ=255)
                                                                                                            gtagtCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTc                                        <  7:179683/149‑1 (MQ=255)
                                                                                                               gtCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTgg                                     <  8:111131/149‑1 (MQ=255)
                                                                                                                tCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGCATCCTGACGTTGCTCACCGTTCTGGt                                    >  5:107471/1‑149 (MQ=255)
                                                                                                                  tGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGc                                  >  7:213980/1‑149 (MQ=255)
                                                                                                                    ccGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGtt                                         <  6:184483/140‑1 (MQ=255)
                                                                                                                    ccGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGtt                                         >  5:184483/1‑140 (MQ=255)
                                                                                                                    ccGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTc                                        >  4:160950/1‑141 (MQ=255)
                                                                                                                    ccGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTc                                        <  3:160950/141‑1 (MQ=255)
                                                                                                                     cgcgTCCACAGCCGGGCAGCGCCAAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGa                               <  8:195205/149‑1 (MQ=255)
                                                                                                                          ccACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGt                                                                 >  7:255028/1‑110 (MQ=255)
                                                                                                                          ccACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGt                                                                 <  8:255028/110‑1 (MQ=255)
                                                                                                                           cacaGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGCGTCGAATCCTGACGTTGCTCACCGGTCTGGTGCTGACATTgg                         >  5:215556/1‑149 (MQ=255)
                                                                                                                           cacaGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTgg                         >  7:153740/1‑149 (MQ=255)
                                                                                                                           cacaGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTg                          <  8:153740/148‑1 (MQ=255)
                                                                                                                              gagtgggCAGTGCCGGTTCGGCGCTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTggcgg                      >  7:55085/5‑149 (MQ=255)
                                                                                                                               gCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTg                                      <  7:165588/132‑1 (MQ=255)
                                                                                                                               gCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTg                                      >  8:165588/1‑132 (MQ=255)
                                                                                                                                            ggttcGGCGCTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATCGGCGGGCGGCCTGGCacac        <  8:55085/145‑1 (MQ=255)
                                                                                                                                               ccGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTgg                         <  1:79371/129‑1 (MQ=255)
                                                                                                                                               ccGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTgg                         >  2:79371/1‑129 (MQ=255)
                                                                                                                                                cGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTGGCGGGCGGCCTGGCACACGcc     >  3:3212/1‑148 (MQ=255)
                                                                                                                                                 ggCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTGGCGGGCGGCCTGGCACACGcc     >  8:119442/1‑147 (MQ=255)
                                                                                                                                                  gCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTGGCGGGCGGCCTGGCACACGCCgag  <  2:212592/149‑1 (MQ=255)
                                                                                                                                                  gCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCCTTCTGGTGCTGACATTGGCGGGCGGCCTGGCACACGCCgac  >  1:212592/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTGCTGGCACGCGCCGACGCCTCGCAGTCGGCCGCCATCGCTTTTCGTTTGGGCGCCGCCGA‑GGCCGCGCGCCTGGCCGCGCAGACGCATTTCGAACTGGTCGGCAGTAGTCTGCCGCGTCCACAGCCGGGCAGCGCCGAACCGGCACTGCCCACTCAACGGCGACTGCTCGGCGACCACTACCTCAACGGCGCCGCCTTGGCAGATGAGCAAAGGGCGCAGGCATGAGTCGAATCCTGACGTTGCTCACCGTTCTGGTGCTGACATTGGCGGGCGGCCTGGCACACGCCGAG  >  NC_002947/3140859‑3141152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑