Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,142,901 G→T 8.2% G284C (GGC→TGC)  PP_2758 → ribose ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,142,9010GT 8.2% 152.5 / 4.1 49G284C (GGC→TGC) PP_2758ribose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base T (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

AAAGGTGAGCTCGACGCGATCTGGGCGGCCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGT  >  NC_002947/3142757‑3143046
                                                                                                                                                  |                                                                                                                                                  
aaaGGTGAACTCGACGCGATCTGGGCGGCCTGGGGTCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCcag‑cccggcag                                                                                                                                                >  1:15043/1‑149 (MQ=255)
       aacTCGACGCGATCTGGGCGGCCTGGGCTCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGt                                                                                                                                          <  2:15043/146‑1 (MQ=255)
         cTCGACGCGATCTGGGCGGCCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGc                                                                                                                                       <  2:262902/149‑1 (MQ=255)
            gACGCGATCTGGGCGGCCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTg                                                                                                                                     <  6:84350/148‑1 (MQ=255)
               gcgATCTGGGCGGCCTGGGATCTGCCGCAGCTCGGTGCAAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑ACAGGCAGCCCCGTGGCTGCAgt                                                                                                                                 >  6:57734/1‑149 (MQ=255)
                      gggCGGCCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGgcg                                                                                                                          >  6:110407/1‑149 (MQ=255)
                           gcCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAgtggtg                                                                                                                             >  4:73165/1‑141 (MQ=255)
                           gcCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAgtggtg                                                                                                                             <  3:73165/141‑1 (MQ=255)
                               gggATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAC‑CACTGCAGCCACCGGGCTGCCGGGCTGGCgcag                                                                                                                       <  8:38748/143‑1 (MQ=25)
                               gggATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAC‑CACTGCAGCCACCGGGCTGCCGGGCTGGCgcag                                                                                                                       >  7:38748/1‑143 (MQ=25)
                                       ccGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAGCCCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGt                                                                                                             <  1:243251/146‑1 (MQ=255)
                                         gCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGCGCGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATc                                                                                                        >  1:222801/1‑149 (MQ=255)
                                              tCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGt                                                                                                  <  2:165955/149‑1 (MQ=255)
                                                      agccagGCGCTTATCGAAGC‑CGGACGCAATGAAGTTCGCACCAATCCCGTCGACGGTACGCCCGAGGTGCTGCAAATGCAACGCCAG‑CCCGGAAGCCCGGAAGCTGCACTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGt                                                                                          >  7:10425/1‑149 (MQ=255)
                                                      agccagGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGt                                                                                          >  2:105650/1‑149 (MQ=255)
                                                      agccagGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGt                                                                                          <  1:105650/149‑1 (MQ=255)
                                                      agccagGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGt                                                                                          >  2:96571/1‑149 (MQ=255)
                                                        ccagGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTg                                                                                         >  2:85222/1‑148 (MQ=255)
                                                            gcgcTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCAt                                                                                      >  2:84841/1‑147 (MQ=255)
                                                            gcgcTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCAt                                                                                      <  1:84841/147‑1 (MQ=255)
                                                                  aTCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCg                                                                               <  3:24441/148‑1 (MQ=255)
                                                                     gAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGccc                                                                            <  5:57734/148‑1 (MQ=255)
                                                                         c‑cGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTAc                                                                      <  1:85222/149‑1 (MQ=255)
                                                                               cGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGc                                                                 <  5:110407/149‑1 (MQ=255)
                                                                                   aTGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CGACCTGGCCGGc                                                             >  4:49952/1‑149 (MQ=255)
                                                                                        gTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCa                                                        >  6:63809/1‑149 (MQ=255)
                                                                                          gcgcACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCAc                                                       >  1:218311/1‑148 (MQ=255)
                                                                                                 tATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCGCGATCTgcc                                               >  7:121850/1‑149 (MQ=255)
                                                                                                 tATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGAGTTGATCgg                                                                                                      <  2:64908/95‑1 (MQ=255)
                                                                                                 tATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGAGTTGATCgg                                                                                                      >  1:64908/1‑95 (MQ=255)
                                                                                                   tGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTgccgc                                             >  3:172142/1‑149 (MQ=255)
                                                                                                     gCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCTCGATCTGCCGccc                                           <  8:121850/149‑1 (MQ=255)
                                                                                                     gCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTgccgcc                                            <  5:63809/148‑1 (MQ=255)
                                                                                                                 cGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTtcgtc                               <  2:218311/149‑1 (MQ=255)
                                                                                                                 cGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTtcgtc                               <  2:222801/149‑1 (MQ=255)
                                                                                                                  gCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGa                                                           <  3:169986/120‑1 (MQ=255)
                                                                                                                  gCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGa                                                           >  4:169986/1‑120 (MQ=255)
                                                                                                                            cTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCctgct                    >  1:73487/1‑149 (MQ=255)
                                                                                                                             tGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCTGAAACCTTCGTCGAGACCctgct                    >  1:56849/1‑148 (MQ=255)
                                                                                                                             tGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCtt                                   <  7:118267/133‑1 (MQ=255)
                                                                                                                             tGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCctgctg                   >  5:354194/1‑149 (MQ=255)
                                                                                                                               gAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCGCCTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGa                  <  1:96571/149‑1 (MQ=255)
                                                                                                                               aggctgctgcGCCAC‑CACTGCAGCCACCGGGCTGCCGGGCTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGaaa                                       >  4:293093/5‑127 (MQ=21)
                                                                                                                               aggctgctgcGCCAC‑CACTGCAGCCACCGGGCTGCCGGGCTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGaaa                                       <  3:293093/123‑1 (MQ=21)
                                                                                                                                aaTTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGAcc                >  3:146467/1‑149 (MQ=255)
                                                                                                                                        cgcCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCAcggcgg         <  4:172142/148‑1 (MQ=255)
                                                                                                                                        cgcCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCAcggcg          <  2:73487/147‑1 (MQ=255)
                                                                                                                                        cgcCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGACCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGAcc                         >  2:58729/1‑132 (MQ=255)
                                                                                                                                        cgcCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGACCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGAcc                         <  1:58729/132‑1 (MQ=255)
                                                                                                                                          ccAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGc       >  8:7116/1‑148 (MQ=255)
                                                                                                                                           cag‑cccggcagcccggTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCaa     >  1:284182/1‑149 (MQ=255)
                                                                                                                                               cccggcagcccggTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGt  <  6:354194/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAAGGTGAGCTCGACGCGATCTGGGCGGCCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGT  >  NC_002947/3142757‑3143046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑