Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,142,922 G→C 7.8% V291L (GTG→CTG)  PP_2758 → ribose ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,142,9220GC 7.8% 161.2 / 4.6 51V291L (GTG→CTG) PP_2758ribose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (26/21);  new base C (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

GGGCGGCCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGTCGATGACGTCAAACGCATCC  >  NC_002947/3142779‑3143066
                                                                                                                                                 |                                                                                                                                                 
gggCGGCCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGgcg                                                                                                                                              >  6:110407/1‑149 (MQ=255)
     gcCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAgtggtg                                                                                                                                                 <  3:73165/141‑1 (MQ=255)
     gcCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAgtggtg                                                                                                                                                 >  4:73165/1‑141 (MQ=255)
         gggATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAC‑CACTGCAGCCACCGGGCTGCCGGGCTGGCgcag                                                                                                                                           <  8:38748/143‑1 (MQ=25)
         gggATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAC‑CACTGCAGCCACCGGGCTGCCGGGCTGGCgcag                                                                                                                                           >  7:38748/1‑143 (MQ=25)
                 ccGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAGCCCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGt                                                                                                                                 <  1:243251/146‑1 (MQ=255)
                   gCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGCGCGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATc                                                                                                                            >  1:222801/1‑149 (MQ=255)
                        tCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGt                                                                                                                      <  2:165955/149‑1 (MQ=255)
                                agccagGCGCTTATCGAAGC‑CGGACGCAATGAAGTTCGCACCAATCCCGTCGACGGTACGCCCGAGGTGCTGCAAATGCAACGCCAG‑CCCGGAAGCCCGGAAGCTGCACTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGt                                                                                                              >  7:10425/1‑149 (MQ=255)
                                agccagGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGt                                                                                                              <  1:105650/149‑1 (MQ=255)
                                agccagGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGt                                                                                                              >  2:96571/1‑149 (MQ=255)
                                agccagGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGt                                                                                                              >  2:105650/1‑149 (MQ=255)
                                  ccagGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTg                                                                                                             >  2:85222/1‑148 (MQ=255)
                                      gcgcTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCAt                                                                                                          <  1:84841/147‑1 (MQ=255)
                                      gcgcTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCAt                                                                                                          >  2:84841/1‑147 (MQ=255)
                                            aTCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCg                                                                                                   <  3:24441/148‑1 (MQ=255)
                                               gAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGccc                                                                                                <  5:57734/148‑1 (MQ=255)
                                                   c‑cGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTAc                                                                                          <  1:85222/149‑1 (MQ=255)
                                                         cGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGc                                                                                     <  5:110407/149‑1 (MQ=255)
                                                             aTGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CGACCTGGCCGGc                                                                                 >  4:49952/1‑149 (MQ=255)
                                                                  gTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCa                                                                            >  6:63809/1‑149 (MQ=255)
                                                                    gcgcACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCAc                                                                           >  1:218311/1‑148 (MQ=255)
                                                                           tATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCGCGATCTgcc                                                                   >  7:121850/1‑149 (MQ=255)
                                                                           tATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGAGTTGATCgg                                                                                                                          >  1:64908/1‑95 (MQ=255)
                                                                           tATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGAGTTGATCgg                                                                                                                          <  2:64908/95‑1 (MQ=255)
                                                                             tGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTgccgc                                                                 >  3:172142/1‑149 (MQ=255)
                                                                               gCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCTCGATCTGCCGccc                                                               <  8:121850/149‑1 (MQ=255)
                                                                               gCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTgccgcc                                                                <  5:63809/148‑1 (MQ=255)
                                                                                           cGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTtcgtc                                                   <  2:218311/149‑1 (MQ=255)
                                                                                           cGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTtcgtc                                                   <  2:222801/149‑1 (MQ=255)
                                                                                            gCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGa                                                                               <  3:169986/120‑1 (MQ=255)
                                                                                            gCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGa                                                                               >  4:169986/1‑120 (MQ=255)
                                                                                                      cTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCctgct                                        >  1:73487/1‑149 (MQ=255)
                                                                                                       tGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCTGAAACCTTCGTCGAGACCctgct                                        >  1:56849/1‑148 (MQ=255)
                                                                                                       tGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCtt                                                       <  7:118267/133‑1 (MQ=255)
                                                                                                       tGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCctgctg                                       >  5:354194/1‑149 (MQ=255)
                                                                                                         gAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCGCCTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGa                                      <  1:96571/149‑1 (MQ=255)
                                                                                                         aggctgctgcGCCAC‑CACTGCAGCCACCGGGCTGCCGGGCTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGaaa                                                           >  4:293093/5‑127 (MQ=21)
                                                                                                         aggctgctgcGCCAC‑CACTGCAGCCACCGGGCTGCCGGGCTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGaaa                                                           <  3:293093/123‑1 (MQ=21)
                                                                                                          aaTTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGAcc                                    >  3:146467/1‑149 (MQ=255)
                                                                                                                  cgcCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCAcggcgg                             <  4:172142/148‑1 (MQ=255)
                                                                                                                  cgcCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCAcggcg                              <  2:73487/147‑1 (MQ=255)
                                                                                                                  cgcCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGACCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGAcc                                             >  2:58729/1‑132 (MQ=255)
                                                                                                                  cgcCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGACCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGAcc                                             <  1:58729/132‑1 (MQ=255)
                                                                                                                    ccAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGc                           >  8:7116/1‑148 (MQ=255)
                                                                                                                     cag‑cccggcagcccggTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCaa                         >  1:284182/1‑149 (MQ=255)
                                                                                                                         cccggcagcccggTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGt                      <  6:354194/149‑1 (MQ=255)
                                                                                                                                  ccggTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAAc                        >  3:77945/1‑138 (MQ=255)
                                                                                                                                  ccggTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAAc                        <  4:77945/138‑1 (MQ=255)
                                                                                                                                     gTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGTCGATGACGTCa           >  4:48198/1‑148 (MQ=255)
                                                                                                                                          tGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGTCGATGACGTCAAACGCa     >  3:191727/1‑149 (MQ=255)
                                                                                                                                          tGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGTCGATGACGTCAAACGCa     >  7:84089/1‑149 (MQ=255)
                                                                                                                                             aGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGTCGATGACGTCAAACGCATcc  >  6:144115/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGGCGGCCTGGGATCTGCCGCAGCTCGGTGCCAGCCAGGCGCTGATCGAAGC‑CGGACGCAATGAAGTGCGCACCTATGGCGTCGACGGTACGCCCGAGGTGCTGGAATTGCTGCGCCAG‑CCCGGCAGCCCGGTGGCTGCAGTGGTGGCGCAGCAGCCTGCGTTGATCGGCCGTACGGCGGTGCATAACGTCGCCCG‑CTACCTGGCCGGCGAGCACGATCTGCCGCCCGAAACCTTCGTCGAGACCCTGCTGACCACGGCGGGCAACGTCGATGACGTCAAACGCATCC  >  NC_002947/3142779‑3143066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑