Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,148,471 T→C 9.3% S137P (TCC→CCC) ‡ msuE → FMN reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,148,4710TC 9.3% 113.8 / 3.3 43S137P (TCC→CCC) ‡msuEFMN reductase
Reads supporting (aligned to +/- strand):  ref base T (19/20);  new base C (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

GAGGTGCACTGGGTGCGCATCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCT  >  NC_002947/3148324‑3148615
                                                                                                                                                   |                                                                                                                                                
gAGGTGCACTGGGTGCGCATCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTc                                                                                                                                                 >  2:283499/1‑149 (MQ=255)
       aCTGGGTGCGCATCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTc                                                                                                                                                 >  5:217042/1‑142 (MQ=255)
       aCTGGGTGCGCATCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTc                                                                                                                                                 <  6:217042/142‑1 (MQ=255)
             tGCGCATCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCCCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTg                                                                                                                                    <  1:283499/149‑1 (MQ=255)
                  aTCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTc                                                                                                                                 >  4:173378/1‑147 (MQ=255)
                  aTCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTAGGACGCGCGATACACCgg                                                                                                                                        >  3:93798/1‑140 (MQ=255)
                  aTCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTAGGACGCGCGATACACCgg                                                                                                                                        <  4:93798/140‑1 (MQ=255)
                   tCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAg                                                                                                                              <  7:331697/149‑1 (MQ=255)
                                cGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGAc                                                                                                                  <  5:44419/148‑1 (MQ=255)
                                     gCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCAc                                                                                                                           <  8:96328/134‑1 (MQ=255)
                                     gCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCAc                                                                                                                           >  7:96328/1‑134 (MQ=255)
                                      ccTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGAc                                                                                                                  >  1:305458/1‑142 (MQ=255)
                                      ccTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGAc                                                                                                                  <  2:305458/142‑1 (MQ=255)
                                       cTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGa                                                                                                          >  7:199726/1‑149 (MQ=255)
                                        tGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGAt                                                                                                         >  1:186292/1‑149 (MQ=255)
                                          gCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGAt                                                                                                         <  2:186292/147‑1 (MQ=255)
                                           ccGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCAc                                                                                                      >  2:263920/1‑149 (MQ=255)
                                             ggTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCa                                                                                                    <  3:35367/149‑1 (MQ=255)
                                                  gCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTAGGACGCGCGATACACCgggc                                                                                                                                      >  8:234427/1‑108 (MQ=38)
                                                  gCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTAGGACGCGCGATACACCgggc                                                                                                                                      <  7:234427/110‑3 (MQ=38)
                                                        gcgcGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAgg                                                                                         >  7:113616/1‑149 (MQ=255)
                                                                       ggCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCgtggt                                                                            <  8:113616/147‑1 (MQ=255)
                                                                       ggCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCgtggt                                                                            <  1:263920/147‑1 (MQ=255)
                                                                            aCCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTgcc                                                                     >  7:322653/1‑149 (MQ=255)
                                                                              cTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTgccgc                                                                   >  2:153520/1‑149 (MQ=255)
                                                                               tGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTgccgc                                                                   >  6:346612/1‑148 (MQ=255)
                                                                                   aCCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCg                                                              <  1:153520/149‑1 (MQ=255)
                                                                                                         cAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCtt                                        >  1:295238/1‑149 (MQ=255)
                                                                                                           aGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTtga                                      <  2:295238/149‑1 (MQ=255)
                                                                                                              cGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTtgatga                                   <  3:173378/149‑1 (MQ=255)
                                                                                                                    gCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCggtg                                                           >  8:65245/1‑119 (MQ=255)
                                                                                                                    gCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCggtg                                                           <  7:65245/119‑1 (MQ=255)
                                                                                                                    gCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATgatcgat                              >  3:249536/1‑148 (MQ=255)
                                                                                                                     cTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATgatcgat                              <  8:199726/147‑1 (MQ=255)
                                                                                                                      tACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCAc                           >  5:272225/1‑149 (MQ=255)
                                                                                                                           gTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTagcgagc                                                  <  8:132704/121‑1 (MQ=255)
                                                                                                                           gTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGtt                      <  4:249536/149‑1 (MQ=255)
                                                                                                                              ggCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCgt                                                                               >  2:24179/1‑89 (MQ=255)
                                                                                                                              ggCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTgc                    <  5:346612/148‑1 (MQ=255)
                                                                                                                                    ccGGTGGATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCt             <  3:180872/149‑1 (MQ=255)
                                                                                                                                         gtATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATgatcgat                              <  2:18470/127‑1 (MQ=255)
                                                                                                                                         gtATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATgatcgat                              >  1:18470/1‑127 (MQ=255)
                                                                                                                                               cgcgTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTttct  >  8:28448/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GAGGTGCACTGGGTGCGCATCGCCGAACTGTCCGCCAGCCTGGCCGGTTCGCTGGAGCGCGATAGCGCCTCGGCTGACCTGCAACCGCATTTGCAGGCCATCGAGCAAGCCGACCTGCTACTGGTCGGCAGCCCGGTGTATCGCGCGTCCTACACCGGTTTGTTCAAGCACCTGTTCGACCTGGTCGATCACCAGTCGCTCAAGGGCGTGCCCGTGGTGCTTGCCGCCACCGGTGGTAGCGAGCGACATGCCTTGATGATCGATCACCAGTTGCGCCCGCTATTCGCTTTCT  >  NC_002947/3148324‑3148615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑