Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,181,393 T→G 8.5% V197G (GTT→GGT) ‡ PP_2791 → aminoglycoside phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,181,3930TG 8.5% 133.5 / 4.9 47V197G (GTT→GGT) ‡PP_2791aminoglycoside phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (22/21);  new base G (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TGTGCCGGGTAGCGCTGCCGGCCGCGCGCTCAGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCATGCGCCGCGCAGCAGCACCCTGTGCCTGTTGCACCG  >  NC_002947/3181250‑3181540
                                                                                                                                               |                                                                                                                                                   
tgtgCCGGGTAGCGCTGCCGGCCGCGCGCTCAGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCg                                                                                                                                                 >  5:175246/1‑148 (MQ=255)
              cTGCCGGCCGCGCGCTCAGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCGGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGcc                                                                                                                                  >  1:284047/1‑149 (MQ=255)
                 ccggccgCGCGCTCAGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTg                                                                                                                               <  5:225667/149‑1 (MQ=255)
                     ccgCGCGCTCAGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGa                                                                                                                           <  2:332280/149‑1 (MQ=255)
                          cgcTCAGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATc                                                                                                                      <  6:175246/149‑1 (MQ=255)
                           gcTCAGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGGAACGGACAggaa                                                                                                                                         <  3:86743/129‑4 (MQ=255)
                           gcTCAGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGGAACGGACAggaa                                                                                                                                         >  4:86743/1‑126 (MQ=255)
                               aGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGc                                                                                                                 >  1:148044/1‑149 (MQ=255)
                               aGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGc                                                                                                                 >  5:99940/1‑149 (MQ=255)
                                  aCCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTcc                                                                                                                                                  >  4:236544/1‑113 (MQ=255)
                                  aCCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTcc                                                                                                                                                  <  3:236544/113‑1 (MQ=255)
                                        gcCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGGAACGGACAggaag                                                                                                                                        >  1:50828/1‑113 (MQ=255)
                                        gcCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGGAACGGACAggaag                                                                                                                                        <  2:50828/117‑5 (MQ=255)
                                          cGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGc                                                                                                                 <  1:137111/138‑1 (MQ=255)
                                             tCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTAcc                                                                                                   >  3:93882/1‑149 (MQ=255)
                                               cTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTc                                                                                                 >  3:247181/1‑149 (MQ=255)
                                                tCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCAt                                                                                                                       >  3:211322/1‑126 (MQ=255)
                                                tCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCAt                                                                                                                       <  4:211322/126‑1 (MQ=255)
                                                    ggCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGAcc                                                                                                                         >  6:295063/1‑120 (MQ=255)
                                                          gcgcAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGc                                                                                      <  4:247181/149‑1 (MQ=255)
                                                                cTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGc                                                                                <  2:284047/149‑1 (MQ=255)
                                                                cTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGc                                                                                <  6:99940/149‑1 (MQ=255)
                                                                cTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGc                                                                                >  4:302778/1‑149 (MQ=255)
                                                                 tGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGcc                                                                               >  4:64239/1‑149 (MQ=255)
                                                                          aGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTg                                                                      <  3:202126/149‑1 (MQ=255)
                                                                                  gcCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATg                                                              >  5:144582/1‑149 (MQ=255)
                                                                                  gcCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATg                                                              >  3:325770/1‑149 (MQ=255)
                                                                                  gcCAACCTGGCGCGTCTGCAACAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTAt                                                                            >  5:211584/1‑135 (MQ=255)
                                                                                  gcCAACCTGGCGCGTCTGCAACAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTAt                                                                            <  6:211584/135‑1 (MQ=255)
                                                                                    cAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATggg                                                            <  8:146889/149‑1 (MQ=255)
                                                                                     aaCCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATgggg                                                           >  1:182914/1‑149 (MQ=255)
                                                                                      aCCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGc                                                          <  3:302778/149‑1 (MQ=255)
                                                                                                    cATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGc                                              <  2:148044/147‑1 (MQ=255)
                                                                                                    cATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGa                                            <  6:144582/149‑1 (MQ=255)
                                                                                                          gTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGc                                              <  6:197820/141‑1 (MQ=255)
                                                                                                          gTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGc                                              >  5:197820/1‑141 (MQ=255)
                                                                                                          gTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCa                                      >  5:78262/1‑149 (MQ=255)
                                                                                                           tCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCAt                                     >  7:205752/1‑149 (MQ=255)
                                                                                                                          ccACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCATGCGCCGCgcagcag                       >  7:11707/1‑148 (MQ=255)
                                                                                                                           cACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCATGCGCCGCgcagcagca                     <  3:64239/149‑1 (MQ=255)
                                                                                                                           cACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCATGCGCCGCgcagcagca                     <  6:78262/149‑1 (MQ=255)
                                                                                                                               cTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGCTGCCTATCCGGTGCTGGAATGGGGCCTgc                                                      >  5:23706/1‑112 (MQ=255)
                                                                                                                               cTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTgc                                                      <  6:23706/112‑1 (MQ=255)
                                                                                                                               cTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCATGCGCCGCGCAGCAGCACCCt                 <  8:11707/149‑1 (MQ=255)
                                                                                                                                tgtgCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCATGCGCCGCGCAGCAGCACCCtg                >  5:244618/1‑149 (MQ=255)
                                                                                                                                       ccTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCATGCGCCGCGCAGCAGCACCCTGTGCCt           <  8:205752/147‑1 (MQ=255)
                                                                                                                                              gTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCATGCGCCGCGCAGCAGCACCCTGTGCCTGTTGCACCg  >  3:69102/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TGTGCCGGGTAGCGCTGCCGGCCGCGCGCTCAGCACCGGCGCCGGTCCTCAGGGCCGGGCGCAACTGGCGACGCAGCTTGGCGCCAACCTGGCGCGTCTGCATCAGGTCCGCCCGCCGTGCGCCACGCTGTGCTTCCTGTCCGTTCCGGACAGCTCGCCGGCCCTGGCGACCATCGACGCCTACCGCCGCTACCTCGACACCCTCGCCGATGCCTATCCGGTGCTGGAATGGGGCCTGCGCTGGTGCGAGCTGCATGCGCCGCGCAGCAGCACCCTGTGCCTGTTGCACCG  >  NC_002947/3181250‑3181540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑