Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,201,399 C→T 6.9% L243L (CTG→TTG)  PP_2808 → oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,201,3990CT 6.9% 171.9 / 2.1 58L243L (CTG→TTG) PP_2808oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (30/24);  new base T (2/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

TCCAGGGAAGTACATCGGCGGCCTCCGGCGCGCGGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAGGGCGGGAAGGGATCATCACGCC  >  NC_002947/3201251‑3201544
                                                                                                                                                    |                                                                                                                                                 
tCCAGGGAAGTACATCGGCGGCCTCCGGCGCGCGGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGc                                                                                                                                                   <  3:168671/149‑1 (MQ=255)
  cAGGGAAGTACATCGGCGGCCTCCGGCGCGCGGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTg                                                                                                                                                 <  5:322185/149‑1 (MQ=255)
         gTACATCGGCGGCCTCCGGCGCGCGGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGcaacc                                                                                                                                           >  7:266700/1‑148 (MQ=255)
           aCATCGGCGGCCTCCGGCGCGCGGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCACGGTGATGGTGCTGGcaacca                                                                                                                                          >  8:278498/1‑147 (MQ=255)
                 gcggcCTCCGGCGCGCGGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGTTGGCAACCAACGCCta                                                                                                                                   >  8:297248/1‑148 (MQ=255)
                 gcggcCTCCGGCGCGCGGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGTTGGCAACCAACGCCta                                                                                                                                   >  8:292521/1‑148 (MQ=255)
                                cgGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTgggg                                                                                                                   <  7:278498/149‑1 (MQ=255)
                                cgGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTgggg                                                                                                                   <  3:347915/149‑1 (MQ=255)
                                  gcaaTCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGgt                                                                                                                 >  7:223750/4‑149 (MQ=255)
                                  gCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGgt                                                                                                                 >  5:272819/1‑149 (MQ=255)
                                  gCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGgt                                                                                                                 <  8:266700/149‑1 (MQ=255)
                                    cATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGgtgt                                                                                                               >  8:165875/1‑149 (MQ=255)
                                     aTCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGtt                                                                                                              <  7:165875/149‑1 (MQ=255)
                                      tCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTg                                                                                                             >  6:12186/1‑149 (MQ=255)
                                        gAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTgc                                                                                                            <  5:358666/148‑1 (MQ=255)
                                                         ctACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGa                                                                                          >  5:59624/1‑149 (MQ=255)
                                                             gAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCa                                                                                                                          <  3:308164/113‑1 (MQ=255)
                                                             gAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCa                                                                                                                          >  4:308164/1‑113 (MQ=255)
                                                               acgcacgcCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCgg                                                                                     >  5:141136/1‑148 (MQ=255)
                                                               acgcacgcCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCgg                                                                                     <  6:141136/148‑1 (MQ=255)
                                                                cgcacgcCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGgt                                                                                   >  6:150117/1‑149 (MQ=255)
                                                                         ttGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAAt                                                                          <  6:59624/149‑1 (MQ=255)
                                                                             tGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTgggg                                                                                                                   <  8:185480/104‑1 (MQ=255)
                                                                             tGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTgggg                                                                                                                   >  7:185480/1‑104 (MQ=255)
                                                                             tGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGTCAATCGa                                                                       >  4:197159/1‑148 (MQ=255)
                                                                               aGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGTTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGaaa                                                                     <  7:292521/148‑1 (MQ=255)
                                                                               aGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGTTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGaaa                                                                     <  7:297248/148‑1 (MQ=255)
                                                                                 cTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAAGTCGCGGCGATTCGGGTGTCGGCAATCGAAAcc                                                                   >  6:36298/1‑148 (MQ=255)
                                                                                  ttCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTcgc                                                                                               <  1:231135/119‑1 (MQ=255)
                                                                                  ttCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTcgc                                                                                               >  2:231135/1‑119 (MQ=255)
                                                                                     aGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAAc                                                              <  8:223750/149‑1 (MQ=255)
                                                                                          aGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAAt                                                                          >  1:42699/1‑132 (MQ=255)
                                                                                          aGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAAt                                                                          <  2:42699/132‑1 (MQ=255)
                                                                                                ggTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACgc                                                   <  6:272819/149‑1 (MQ=255)
                                                                                                     ttACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGcagcag                                              <  5:150117/149‑1 (MQ=255)
                                                                                                        ccTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTg                                           >  1:19258/1‑149 (MQ=255)
                                                                                                         cTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTgggg                                                                                                                   <  6:140384/76‑1 (MQ=255)
                                                                                                         cTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTgggg                                                                                                                   >  5:140384/1‑76 (MQ=255)
                                                                                                           gCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCt                                        >  2:318596/1‑149 (MQ=255)
                                                                                                            cAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAg                                                                                                    <  1:54115/88‑1 (MQ=255)
                                                                                                            cAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAg                                                                                                    >  2:54115/1‑88 (MQ=255)
                                                                                                            cAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGt                                                         >  4:85181/1‑131 (MQ=255)
                                                                                                             aaGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGc                                      <  3:197159/149‑1 (MQ=255)
                                                                                                                    cccATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGc                               >  8:210634/1‑149 (MQ=255)
                                                                                                                        tGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAAGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGc                           <  5:36298/149‑1 (MQ=255)
                                                                                                                           ccGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAg                        <  1:318596/149‑1 (MQ=255)
                                                                                                                            cGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAgg                       >  1:59707/1‑149 (MQ=255)
                                                                                                                             gTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAggg                      >  5:249758/1‑149 (MQ=255)
                                                                                                                             gTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAggg                      <  5:12186/149‑1 (MQ=255)
                                                                                                                               gCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAGGGCg                    >  7:267373/1‑149 (MQ=255)
                                                                                                                                cAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAGGGCgg                   >  6:203778/1‑149 (MQ=255)
                                                                                                                                   ccGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAGGGCggg                  >  8:22220/1‑147 (MQ=255)
                                                                                                                                    cGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTAGACGCAGCAGTTGGATGCCGTCGGCTGGCAAGGGCGGGAAg               >  8:49802/1‑149 (MQ=255)
                                                                                                                                      cccaGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAGGGCGGGAAggg             <  7:49802/149‑1 (MQ=255)
                                                                                                                                           gTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAGGGCGGGAAGGGatcat        >  5:23731/1‑149 (MQ=255)
                                                                                                                                              aTGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAGGGCGGGAAGGGATCATCAc     >  6:109289/1‑149 (MQ=255)
                                                                                                                                                 gTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAGGGCGGGAAGGGATCATCAcgcc  <  5:109289/149‑1 (MQ=255)
                                                                                                                                                  tGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACgc                                                   <  8:201512/99‑1 (MQ=255)
                                                                                                                                                  tGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACgc                                                   >  7:201512/1‑99 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCCAGGGAAGTACATCGGCGGCCTCCGGCGCGCGGCCATCGAGGCGGGGGTGAAGCTCTACGAACGCACGCCGTTGCTGAGCTTCAGCGAAGGGCCGGTCATTACCTGCAAGACTGCCCATGGCCGTGCAACCGCCCCGGTGATGGTGCTGGCAACCAACGCCTATACCCCGCAGCTGGGGGTGTTGCGCAACAAGGTCGCGGCGATTCGGGTGTCGGCAATCGAAACCCAAACGCTGTCGACGCAGCAGTTGGCTGCCCTCGGCTGGCAAGGGCGGGAAGGGATCATCACGCC  >  NC_002947/3201251‑3201544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑