Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,206,359 G→A 8.1% G3D (GGT→GAT)  PP_2812 → transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,206,3590GA 8.1% 138.2 / 2.2 49G3D (GGT→GAT) PP_2812transporter
Reads supporting (aligned to +/- strand):  ref base G (21/24);  new base A (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GAATTTCGTACGAAACCCGGCCAGTTGGCAGATTTCCCTCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGCGGTGTTCACGCTGATCATGGGCTGGTTCGCCGTGCAACTGCGCATGGATGCT  >  NC_002947/3206211‑3206507
                                                                                                                                                    |                                                                                                                                                    
gAATTTCGTACGAAACCCGGCCAGTTGGCAGATTTCCCTCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACgg                                                                                                                                                      >  6:192680/1‑149 (MQ=255)
          cGAAACCCGGCCAGTTGGCAGATTTCCCTCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAg                                                                                                                                            <  6:184135/149‑1 (MQ=255)
                ccGGCCAGTTGGCAGATTTCCCTCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCt                                                                                                                                      >  4:324266/1‑149 (MQ=255)
                 cGGCCAGTTGGCAGATTTCCCTCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTg                                                                                                                                     <  6:328778/149‑1 (MQ=255)
                   gCCAGTTGGCAGATTTCCGTCAACTACGCGAAGTTCATGGGCTGCGAAACCCCATACGTCCAGCTTTATCGTGACCGCGATTATGTCGGCATCTCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGaa                                                                                                                                   <  3:245134/149‑1 (MQ=255)
                          ggCAGATTTCCCTCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCg                                                                                                                             <  5:146277/148‑1 (MQ=255)
                                    ccTCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCt                                                                                                                  >  3:321102/1‑149 (MQ=255)
                                      tCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTTCGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGATTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCtgt                                                                                                                >  5:105988/1‑149 (MQ=255)
                                      tCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTTCGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGATTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCtgt                                                                                                                >  5:101015/1‑149 (MQ=255)
                                      tCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCtgt                                                                                                                >  8:295064/1‑149 (MQ=255)
                                      tCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGACCtgt                                                                                                                >  5:121585/1‑149 (MQ=255)
                                                   gTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGa                                                                                                   <  3:324266/149‑1 (MQ=255)
                                                    ttCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAg                                                                                                  <  7:295064/149‑1 (MQ=255)
                                                     tCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCAATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAgg                                                                                                 >  5:305405/1‑149 (MQ=255)
                                                      cATGGGCGGCGAAACCCCCTTCGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGATTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAgg                                                                                                 <  6:101015/148‑1 (MQ=255)
                                                      cATGGGCGGCGAAACCCCCTTCGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGATTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAgg                                                                                                 <  6:105988/148‑1 (MQ=255)
                                                      cATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAgg                                                                                                 <  6:305405/148‑1 (MQ=255)
                                                       aTGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCtg                                                                                                                 >  5:274913/1‑131 (MQ=255)
                                                       aTGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCtg                                                                                                                 <  6:274913/131‑1 (MQ=255)
                                                         gggCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGgtgt                                                                                             >  1:169/1‑149 (MQ=255)
                                                          ggcggcGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGgtgt                                                                                             <  5:192680/148‑1 (MQ=255)
                                                             ggcgAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCAc                                                                                                          >  2:158181/1‑132 (MQ=255)
                                                             ggcgAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCAc                                                                                                          <  1:158181/132‑1 (MQ=255)
                                                                     ccccTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGgtgtg                                                                                            <  4:81675/138‑1 (MQ=255)
                                                                     ccccTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGgtgtg                                                                                            >  3:81675/1‑138 (MQ=255)
                                                                        cTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTc                                                                                                     >  5:203346/1‑126 (MQ=255)
                                                                        cTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTc                                                                                                     <  6:203346/126‑1 (MQ=255)
                                                                           cGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGACCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCAtcgtc                                                                            <  6:121585/148‑1 (MQ=255)
                                                                              ccAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCgg                                                                                    <  8:34963/137‑1 (MQ=255)
                                                                              ccAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCgg                                                                                    >  7:34963/1‑137 (MQ=255)
                                                                                            aCCGCGATTATGTCGGCATCGCGGTTTACAGCACCTTGTGAGGCCTGACGTGAACGGGTCTGTAAAGCTATCTGAAGCCCAGGAACGTTGCCTGTTGTCACGCGTCGAGGGGTTGTTGTTCGGGCATCGTAGTCTGGTGATGgagacca                                                          >  4:104513/1‑146 (MQ=255)
                                                                                             ccGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCt                                                         <  4:321102/149‑1 (MQ=255)
                                                                                              cgcgATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGc                                                           >  4:182423/1‑146 (MQ=255)
                                                                                              cgcgATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGc                                                           <  3:182423/146‑1 (MQ=255)
                                                                                                     aTGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCgg                                                                                    <  4:13607/114‑1 (MQ=255)
                                                                                                     aTGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCgg                                                                                    >  3:13607/1‑114 (MQ=255)
                                                                                                     aTGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGGCTGGTGCTGGCGAGCCTGGCGgtgt                                                 <  3:139827/149‑1 (MQ=255)
                                                                                                              tCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGCGGTGTTCACGCTGa                                        >  1:334882/1‑149 (MQ=255)
                                                                                                                  gATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGCGGTGTTCACGCTGATCAt                                    <  2:169/149‑1 (MQ=255)
                                                                                                                        ccGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGgtgtg                                                                                            <  4:43467/87‑1 (MQ=255)
                                                                                                                        ccGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGgtgtg                                                                                            >  3:43467/1‑87 (MQ=255)
                                                                                                                        ccGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCgg                                                                                    <  4:99620/95‑1 (MQ=255)
                                                                                                                        ccGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCgg                                                                                    >  3:99620/1‑95 (MQ=255)
                                                                                                                        ccGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGc                                                      <  2:164485/125‑1 (MQ=255)
                                                                                                                        ccGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGc                                                      >  1:164485/1‑125 (MQ=255)
                                                                                                                        ccGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGCGGTGTTCACGCTGATCATGGGCTg                              >  7:202870/1‑149 (MQ=255)
                                                                                                                        ccGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGCGGTGTTCACGCTGATCATGGGCTg                              <  8:202870/149‑1 (MQ=255)
                                                                                                                                           ccGTGAACGGTTCTGTATAGCTTTCTTAAGCGCGGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCTTCGTCGTCTGGTGCTTGCGAGCCTGGCGGTGTTCACGCTGATCATGGGCTGGTTCGCCGTGCAACTgc             <  3:104513/147‑1 (MQ=255)
                                                                                                                                             gTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGTTGGCGAGCCTGGCGGTGTTCACGCTGATCATGGGCTGGTTCGCCGTGCAACTGCGCAt         >  4:91310/1‑149 (MQ=255)
                                                                                                                                              tGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGCGGTGTTCACGCTGATCATGGGCTGGTTCGCCGTGCAACTGCGCATg        >  5:97786/1‑149 (MQ=255)
                                                                                                                                                   ggTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGCGGTGTTCACGCTGATCATGGGCTGGTTCGCCGTGCAACTGCGCATGGATGc   >  2:124080/1‑149 (MQ=255)
                                                                                                                                                    gTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGCGGTGTTCACGCTGATCATGGGCTGGTTCGCCGTGCAACTGCGCATGGATGCt  >  7:323230/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAATTTCGTACGAAACCCGGCCAGTTGGCAGATTTCCCTCAACTACGCGAAGTTCATGGGCGGCGAAACCCCCTACGACCAGCTTTATCGTGACCGCGATTATGTCGGCATCGCGATTTCCCGCACCTTGTGAGGCCTGCCGTGAACGGTTCTGTAAAGCTATCTGAAGCCCCGGCACGTGGCCTGTTGTCACGCGTCGAGGGTGTGTTGTTCGGGCATCGTCGTCTGGTGCTGGCGAGCCTGGCGGTGTTCACGCTGATCATGGGCTGGTTCGCCGTGCAACTGCGCATGGATGCT  >  NC_002947/3206211‑3206507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑