Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,210,076 T→G 11.9% N217T (AAC→ACC)  PP_2814 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,210,0760TG11.9% 155.0 / 14.3 59N217T (AAC→ACC) PP_2814hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/26);  new base G (3/4);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

GCGGAAACCGAGGATCTTCTGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTT  >  NC_002947/3209931‑3210223
                                                                                                                                                 |                                                                                                                                                   
gCGGAAACCGAGGATCTTCTGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTgg                                                                                                                                                  >  7:200063/1‑149 (MQ=255)
  ggAAACCGAGGATCTTCTGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGcc                                                                                                                                                >  4:189261/1‑149 (MQ=255)
        cGAGGATCTTCTGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCa                                                                                                                                          >  6:170821/1‑149 (MQ=255)
           ggATCTTCTGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATCGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCg                                                                                                                                       <  8:224377/149‑1 (MQ=255)
                ttctGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAAc                                                                                                                                  >  3:233671/1‑149 (MQ=255)
                  ctGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGGTGGCGGGGCCAACGTGa                                                                                                                                    <  7:5285/145‑1 (MQ=255)
                   tGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGAc                                                                                                                               >  6:326297/1‑149 (MQ=255)
                     tACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGAc                                                                                                                               <  3:189261/147‑1 (MQ=255)
                     tACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGGTGGCGGGGCCAACGTGaccacc                                                                                                                               <  7:28674/147‑6 (MQ=255)
                       cccGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTg                                                                                                                           >  3:39701/1‑149 (MQ=255)
                        ccGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTgc                                                                                                                          >  3:284181/1‑149 (MQ=255)
                                aaaGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAAc                                                                                                                  <  5:170821/149‑1 (MQ=255)
                                 aaGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACa                                                                                                                 <  2:241631/149‑1 (MQ=255)
                                      tGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACg                                                                                                            <  4:39701/149‑1 (MQ=255)
                                         gcACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCa                                                                                                         >  6:118823/1‑149 (MQ=255)
                                                   gAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTgccgc                                                                                                                       <  6:87320/125‑1 (MQ=255)
                                                   gAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTgccgc                                                                                                                       >  5:87320/1‑125 (MQ=255)
                                                   gAGCGGCACGATCAACGGGTAGGCTTCTACAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCACCCCACCCTGAACGACCTTGCCGCCGAACAGCACGTCATACCCGGCtt                                                                                               >  8:249411/1‑149 (MQ=255)
                                                      cGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGa                                                                                            <  3:338292/149‑1 (MQ=255)
                                                      cGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGa                                                                                            <  5:326297/149‑1 (MQ=255)
                                                            gATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAgcaggg                                                                                      <  4:154489/149‑1 (MQ=255)
                                                              tCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGAACGGCATCCCCGGCTTTGAgcagggca                                                                                    <  8:200063/149‑1 (MQ=255)
                                                                aaCGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGGTGGCGGGGCCAACGTGa                                                                                                                                    <  6:262845/99‑1 (MQ=255)
                                                                aaCGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGGTGGCGGGGCCAACGTGa                                                                                                                                    >  5:262845/1‑99 (MQ=255)
                                                                     gTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCg                                                                             <  4:233671/149‑1 (MQ=255)
                                                                           ttcttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCAtc                                                                        >  3:325136/1‑148 (MQ=255)
                                                                           ttcttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCAtc                                                                        >  1:279985/1‑148 (MQ=255)
                                                                           ttcttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAAACGTCAtct                                                                       >  2:28350/1‑149 (MQ=255)
                                                                              ttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGc                                                                                                          <  8:39935/111‑1 (MQ=255)
                                                                              ttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTg                                                                    >  4:269523/1‑149 (MQ=255)
                                                                                        aTTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGa                                                           <  3:198983/148‑1 (MQ=255)
                                                                                         ttATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACgg                                                                                                           <  8:330188/99‑1 (MQ=255)
                                                                                         ttATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACAGTGAACGACCTTGCCGCCGAACAGCACgg                                                                                                           >  7:330188/1‑99 (MQ=255)
                                                                                              gAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACAtcgtc                                                    >  3:51534/1‑149 (MQ=255)
                                                                                              gAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACAtcgt                                                     <  4:284181/148‑1 (MQ=255)
                                                                                                 gCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGaa                                                 <  2:148002/149‑1 (MQ=255)
                                                                                                 gCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGaa                                                 <  5:118823/149‑1 (MQ=255)
                                                                                                 gCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGGCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAAACGTCATCTCTGGGGTGCCGACATCGTCGaa                                                 <  1:28350/149‑1 (MQ=255)
                                                                                                   cTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGaa                                                 >  1:258142/1‑147 (MQ=255)
                                                                                                   cTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGaa                                                 <  2:258142/147‑1 (MQ=255)
                                                                                                   cTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGa                                                  >  5:175034/1‑146 (MQ=255)
                                                                                                   cTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGa                                                  <  6:175034/146‑1 (MQ=255)
                                                                                                        tCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAgcag                                                                                        <  3:294685/103‑1 (MQ=255)
                                                                                                        tCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAgcag                                                                                        >  4:294685/1‑103 (MQ=255)
                                                                                                           ggATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAg                                       >  5:64714/1‑149 (MQ=255)
                                                                                                                  cAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAg                                                <  5:32221/133‑1 (MQ=255)
                                                                                                                  cAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAg                                                >  6:32221/1‑133 (MQ=255)
                                                                                                                      ccGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTccc                            <  2:279985/149‑1 (MQ=255)
                                                                                                                       cgcgACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCg                           >  6:307723/1‑149 (MQ=255)
                                                                                                                        gcgACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCgg                          <  4:51534/149‑1 (MQ=255)
                                                                                                                           aCGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGa                       <  3:269523/149‑1 (MQ=255)
                                                                                                                                aaTAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATc                                                                              >  6:98870/1‑89 (MQ=255)
                                                                                                                                aaTAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAg                  >  3:171525/1‑149 (MQ=255)
                                                                                                                                 aTAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGATCAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGGCATCCCAGTCCTGGCTCCCGGGGATCAAg                  <  7:249411/148‑1 (MQ=255)
                                                                                                                                          gttCACGGTGGCGGGGCCAACGTGACCGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGt                                                                            <  2:250658/79‑1 (MQ=25)
                                                                                                                                          gttCACGGTGGCGGGGCCAACGTGACCGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGt                                                                            >  1:250658/3‑81 (MQ=25)
                                                                                                                                          gttCACGGTGGCGGGGCCAACGTGACCGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGgcgc        >  2:120173/3‑149 (MQ=255)
                                                                                                                                            tCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCg      >  1:18894/1‑149 (MQ=255)
                                                                                                                                                gTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGtttt  >  6:181879/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCGGAAACCGAGGATCTTCTGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTT  >  NC_002947/3209931‑3210223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑