Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,210,088	C→A	10.8%	G213V (GGT→GTT)	PP_2814 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,210,088	0	C	A	10.8%	188.1 / 10.8	64	G213V (GGT→GTT)	PP_2814	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/32);  new base A (3/4);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

GGATCTTCTGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTTCCTCGGT  >  NC_002947/3209942‑3210230                                                                                                                                                   |                                                                                                                                               ggATCTTCTGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATCGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCg                                                                                                                                              <  8:224377/149‑1 (MQ=255)      ttctGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAAc                                                                                                                                         >  3:233671/1‑149 (MQ=255)        ctGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGGTGGCGGGGCCAACGTGa                                                                                                                                           <  7:5285/145‑1 (MQ=255)         tGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGAc                                                                                                                                      >  6:326297/1‑149 (MQ=255)           tACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGAc                                                                                                                                      <  3:189261/147‑1 (MQ=255)           tACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGGTGGCGGGGCCAACGTGaccacc                                                                                                                                      <  7:28674/147‑6 (MQ=255)             cccGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTg                                                                                                                                  >  3:39701/1‑149 (MQ=255)              ccGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTgc                                                                                                                                 >  3:284181/1‑149 (MQ=255)                      aaaGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAAc                                                                                                                         <  5:170821/149‑1 (MQ=255)                       aaGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACa                                                                                                                        <  2:241631/149‑1 (MQ=255)                            tGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACg                                                                                                                   <  4:39701/149‑1 (MQ=255)                               gcACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCa                                                                                                                >  6:118823/1‑149 (MQ=255)                                         gAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTgccgc                                                                                                                              >  5:87320/1‑125 (MQ=255)                                         gAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTgccgc                                                                                                                              <  6:87320/125‑1 (MQ=255)                                         gAGCGGCACGATCAACGGGTAGGCTTCTACAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCACCCCACCCTGAACGACCTTGCCGCCGAACAGCACGTCATACCCGGCtt                                                                                                      >  8:249411/1‑149 (MQ=255)                                            cGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGa                                                                                                   <  3:338292/149‑1 (MQ=255)                                            cGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGa                                                                                                   <  5:326297/149‑1 (MQ=255)                                                  gATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAgcaggg                                                                                             <  4:154489/149‑1 (MQ=255)                                                    tCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGAACGGCATCCCCGGCTTTGAgcagggca                                                                                           <  8:200063/149‑1 (MQ=255)                                                      aaCGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGGTGGCGGGGCCAACGTGa                                                                                                                                           <  6:262845/99‑1 (MQ=255)                                                      aaCGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGGTGGCGGGGCCAACGTGa                                                                                                                                           >  5:262845/1‑99 (MQ=255)                                                           gTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCg                                                                                    <  4:233671/149‑1 (MQ=255)                                                                 ttcttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCAtc                                                                               >  3:325136/1‑148 (MQ=255)                                                                 ttcttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCAtc                                                                               >  1:279985/1‑148 (MQ=255)                                                                 ttcttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAAACGTCAtct                                                                              >  2:28350/1‑149 (MQ=255)                                                                    ttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGc                                                                                                                 <  8:39935/111‑1 (MQ=255)                                                                    ttcAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTg                                                                           >  4:269523/1‑149 (MQ=255)                                                                              aTTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGa                                                                  <  3:198983/148‑1 (MQ=255)                                                                               ttATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACgg                                                                                                                  <  8:330188/99‑1 (MQ=255)                                                                               ttATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACAGTGAACGACCTTGCCGCCGAACAGCACgg                                                                                                                  >  7:330188/1‑99 (MQ=255)                                                                                    gAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACAtcgtc                                                           >  3:51534/1‑149 (MQ=255)                                                                                    gAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACAtcgt                                                            <  4:284181/148‑1 (MQ=255)                                                                                       gCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGaa                                                        <  2:148002/149‑1 (MQ=255)                                                                                       gCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGaa                                                        <  5:118823/149‑1 (MQ=255)                                                                                       gCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGGCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAAACGTCATCTCTGGGGTGCCGACATCGTCGaa                                                        <  1:28350/149‑1 (MQ=255)                                                                                         cTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGaa                                                        <  2:258142/147‑1 (MQ=255)                                                                                         cTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGaa                                                        >  1:258142/1‑147 (MQ=255)                                                                                         cTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGa                                                         <  6:175034/146‑1 (MQ=255)                                                                                         cTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGa                                                         >  5:175034/1‑146 (MQ=255)                                                                                              tCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAgcag                                                                                               >  4:294685/1‑103 (MQ=255)                                                                                              tCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAgcag                                                                                               <  3:294685/103‑1 (MQ=255)                                                                                                 ggATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAg                                              >  5:64714/1‑149 (MQ=255)                                                                                                        cAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAg                                                       <  5:32221/133‑1 (MQ=255)                                                                                                        cAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAg                                                       >  6:32221/1‑133 (MQ=255)                                                                                                            ccGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTccc                                   <  2:279985/149‑1 (MQ=255)                                                                                                             cgcgACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCg                                  >  6:307723/1‑149 (MQ=255)                                                                                                              gcgACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCgg                                 <  4:51534/149‑1 (MQ=255)                                                                                                                 aCGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGa                              <  3:269523/149‑1 (MQ=255)                                                                                                                      aaTAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATc                                                                                     >  6:98870/1‑89 (MQ=255)                                                                                                                      aaTAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAg                         >  3:171525/1‑149 (MQ=255)                                                                                                                       aTAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGATCAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGGCATCCCAGTCCTGGCTCCCGGGGATCAAg                         <  7:249411/148‑1 (MQ=255)                                                                                                                                gttCACGGTGGCGGGGCCAACGTGACCGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGt                                                                                   <  2:250658/79‑1 (MQ=25)                                                                                                                                gttCACGGTGGCGGGGCCAACGTGACCGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGt                                                                                   >  1:250658/3‑81 (MQ=25)                                                                                                                                gttCACGGTGGCGGGGCCAACGTGACCGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGgcgc               >  2:120173/3‑149 (MQ=255)                                                                                                                                  tCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCg             >  1:18894/1‑149 (MQ=255)                                                                                                                                      gTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGtttt         >  6:181879/1‑149 (MQ=255)                                                                                                                                          gCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGcc              >  4:236/1‑140 (MQ=255)                                                                                                                                          gCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGcc              <  3:236/140‑1 (MQ=255)                                                                                                                                          gCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTTCCTc     >  7:70639/1‑149 (MQ=255)                                                                                                                                            cccGCCACCGTGAACGACCTTGCCGCCGGACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTTCCTCgg   <  3:148477/149‑1 (MQ=255)                                                                                                                                            cccGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTTCCTCgg   <  2:18894/149‑1 (MQ=255)                                                                                                                                             ccGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTTCCTCGGt  <  4:171525/149‑1 (MQ=255)                                                                                                                                             ccGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTTCCTCGGt  <  5:181879/149‑1 (MQ=255)                                                                                                                                             ccGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTTCCTCGGt  <  4:325136/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               GGATCTTCTGTACCCGCGGAGAAAGCGTGCGCACCAGCTCGAGCGGCACGATCAACGGGTAGGCTTCTTCAGGCGTGATTATCGAAGCCTGCATCGGGATGGTCAGCCCGCGACGGTAATAGTCGGTGGTCACGTTGGCCCCGCCACCGTGAACGACCTTGCCGCCGAACAGCACGGCATCCCCGGCTTTGAGCAGGGCAGGAATCGTCATCTCTGGGGTGCCGACATCGTCGAAGTCATCCCAGTCCTGGCTCCCGGGGATCAAGCGCGTGGCGCCGTTTTCCTCGGT  >  NC_002947/3209942‑3210230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑