Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,266,111	G→C	9.3%	intergenic (+28/+31)	PP_2863 → / ← PP_2864	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,266,111	0	G	C	9.3%	151.6 / 7.5	54	intergenic (+28/+31)	PP_2863/PP_2864	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/28);  new base C (2/3);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-01

GTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCT  >  NC_002947/3265963‑3266258                                                                                                                                                      |                                                                                                                                                    gTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCggg                                                                                                                                                     >  8:40392/1‑149 (MQ=255)  tttCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCggg                                                                                                                                                     >  1:155589/1‑148 (MQ=255)       gCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGc                                                                                                                                               <  3:333657/149‑1 (MQ=255)         acaAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCcg                                                                                                                                             >  3:225123/1‑149 (MQ=255)               cccGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAg                                                                                                                                       <  5:359338/149‑1 (MQ=255)               cccGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAg                                                                                                                                       <  7:40392/149‑1 (MQ=255)                ccGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGGTGTTTGTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCATTTGTACCGGCATC‑TTCGCGGGTAAAGCCGCGAAAgg                                                                                                                                      >  5:306437/1‑149 (MQ=255)                 cGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCT‑TTCGCGGCTTTACCCGCGaagagg                                                                                                                                     <  6:260994/149‑5 (MQ=38)                   cAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCT‑TTCGCGGCTTTACCCGCGAAGAGGcc                                                                                                                                   <  3:296645/149‑1 (MQ=38)                        tttCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGc                                                                                                                                    <  6:143241/143‑1 (MQ=255)                        tttCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGc                                                                                                                                    >  5:143241/1‑143 (MQ=255)                             ttCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                         >  4:121567/1‑149 (MQ=255)                             ttCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                         >  8:10936/1‑149 (MQ=255)                                 aGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGAt                                                                                                                      >  7:269364/1‑148 (MQ=255)                                   ggggtgGGCGGGGGGTTTGTGCTGCTGGACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATcac                                                                                                                   <  6:306437/147‑1 (MQ=255)                                      gtgtGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGaa                                                                                                                               >  5:85370/1‑134 (MQ=255)                                      gtgtGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGaa                                                                                                                               <  6:85370/134‑1 (MQ=255)                                      gtgtGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACAt                                                                                                                 <  2:155589/148‑1 (MQ=255)                                       tgggCTGGGGGATTGGGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGa                                                                                                               <  7:288623/146‑1 (MQ=255)                                            tGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGt                                                                                                                          >  5:175467/1‑133 (MQ=255)                                            tGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGt                                                                                                                          <  6:175467/133‑1 (MQ=255)                                            tGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                         >  5:290454/1‑134 (MQ=255)                                            tGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                         <  6:290454/134‑1 (MQ=255)                                            tGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                         <  2:24994/134‑1 (MQ=255)                                            tGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                         >  1:24994/1‑134 (MQ=255)                                                   gtgtgCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCt                                                                                                   <  7:8707/148‑1 (MQ=255)                                                         tgctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCT‑TTCGCGGCTTTACCCGCGaagaggc                                                                                                                                    >  7:183725/1‑105 (MQ=25)                                                         tgctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCT‑TTCGCGGCTTTACCCGCGaagaggc                                                                                                                                    <  8:183725/110‑6 (MQ=25)                                                         tgctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCAc                                                                                                     <  2:5483/141‑1 (MQ=255)                                                         tgctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCAc                                                                                                     >  1:5483/1‑141 (MQ=255)                                                              cACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCAc                                                                                                     <  8:275444/136‑1 (MQ=255)                                                                ccAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTggg                                                                                      <  5:142025/149‑1 (MQ=255)                                                                 cAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTggg                                                                                      >  3:337685/1‑148 (MQ=255)                                                                               aGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTAc                                                                       <  7:10936/149‑1 (MQ=255)                                                                                gACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATcac                                                                                                                   <  2:33879/104‑1 (MQ=255)                                                                                   tGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCACCCGCTTGGGCAACCGCCGCACTACCAGc                                                                   >  8:293777/1‑149 (MQ=255)                                                                                       tttttCTTTAGGGGCTTATTTTGCCCCTTGGTTTATGTGCAGTTTAACCGCTATCTTTCGCGGGTAAAGCCCCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTgg                                                                <  1:112185/147‑1 (MQ=255)                                                                                         tttCTTGACGGGCGTAATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTg                                                              <  8:269364/148‑1 (MQ=255)                                                                                             ttGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCAC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTggg                                                                                      <  4:192755/120‑1 (MQ=255)                                                                                             ttGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCAC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTggg                                                                                      >  3:192755/1‑120 (MQ=255)                                                                                                          tCTGCCGCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGAc                                                                              <  8:130867/115‑1 (MQ=255)                                                                                                          tCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCACCCCCACCTGCACCCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCCCTGCCACCCCTc                                            >  7:283913/1‑149 (MQ=255)                                                                                                            tGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTca                                           >  2:163460/1‑148 (MQ=255)                                                                                                             gCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTcaca                                         >  1:229070/1‑149 (MQ=255)                                                                                                              ccccTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCACACCTGCAACCGCTTGGGCAACCGACGCACAACCAGCTGGTGCGACCGCTGAAACACCTcaca                                         >  8:106101/1‑148 (MQ=255)                                                                                                                 cTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCCACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCGGCAACACGTCACACAGt                                     >  7:83008/1‑149 (MQ=255)                                                                                                                    tgTTGATTTGCAGTTGTACAGGCCTC‑TTCGCGCGTAAAGCCGAGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTtc                                   <  8:283913/147‑1 (MQ=255)                                                                                                                     gTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTtcat                                 <  4:225123/149‑1 (MQ=255)                                                                                                                           gtgCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                                        <  2:320174/88‑1 (MQ=255)                                                                                                                           gtgCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                                        >  1:320174/1‑88 (MQ=255)                                                                                                                            tgCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGcc                           <  3:121567/148‑1 (MQ=255)                                                                                                                                   gTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATggg                    <  2:229070/148‑1 (MQ=255)                                                                                                                                      ccGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAg                >  3:34451/1‑149 (MQ=255)                                                                                                                                              tttCGCGGCTTTACCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGgcggcg         >  7:283752/2‑149 (MQ=255)                                                                                                                                                 cgcgGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGgcggcg         <  4:34451/146‑1 (MQ=255)                                                                                                                                                  gcgGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCa      <  1:163460/148‑1 (MQ=255)                                                                                                                                                    gggTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGc   <  7:293777/149‑1 (MQ=255)                                                                                                                                                      gTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCt  >  3:329979/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                    GTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTC‑TTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCT  >  NC_002947/3265963‑3266258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑