Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,268,302 T→G 7.4% K371Q (AAG→CAG)  PP_2867 ← pyridine nucleotide‑disulphide oxidoreductase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,268,3020TG 7.4% 206.5 / 7.2 68K371Q (AAG→CAG) PP_2867pyridine nucleotide‑disulphide oxidoreductase family protein
Reads supporting (aligned to +/- strand):  ref base T (31/32);  new base G (2/3);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

GTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGTTGGTGCTACGCCAAG  >  NC_002947/3268157‑3268440
                                                                                                                                                 |                                                                                                                                           
gTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGCAGCTTCac                                                                                                                                          >  6:230480/1‑148 (MQ=255)
   gCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAgcgc                                                                                                                                       >  7:13990/1‑149 (MQ=255)
       gCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGTGCGTATCATCTGCCGCCAGGGCCGATGCCCGGTCAGCAGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTGAAGCGccaga                                                                                                                                   <  3:317643/149‑4 (MQ=255)
       gCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTg                                                                                                                                   <  5:147111/149‑1 (MQ=255)
              ccTGTCGAAACACAAGGCCACTCGATCCTGTAGAAGCGTTCAGGCCACCGGGATTAGCGGATGAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCGACACTGAATTGATCTGGCGCTGAAGCGccag                                                                                                                                    <  3:324694/141‑3 (MQ=255)
              ccTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCgg                                                                                                                            >  4:310942/1‑149 (MQ=255)
              ccTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACAGAATTGATCTGGCTCTGAAGCGccag                                                                                                                                    >  4:324694/1‑139 (MQ=255)
                 gTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCg                                                                                                                             <  5:343913/145‑1 (MQ=255)
                 gTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCg                                                                                                                             <  8:132997/145‑1 (MQ=255)
                 gTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCg                                                                                                                             >  7:132997/1‑145 (MQ=255)
                 gTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCg                                                                                                                             >  6:343913/1‑145 (MQ=255)
                  tCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCtt                                                                                                                        <  6:95337/149‑1 (MQ=255)
                    gAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGt                                                                                                                      >  8:163060/1‑149 (MQ=255)
                          caAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCg                                                                                                                <  2:306228/149‑1 (MQ=255)
                           aaGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTAGGCCTTGTCCTCCgg                                                                                                               >  5:33893/1‑149 (MQ=255)
                           aaGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGAGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTGAAGCGccag                                                                                                                                    >  7:250133/1‑126 (MQ=255)
                           aaGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGAGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTGAAGCGccag                                                                                                                                    <  8:250133/128‑3 (MQ=255)
                               ccACTCCAACCTGTAGAAGCGTTCGGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGc                                                                                                           <  1:322711/149‑1 (MQ=255)
                                cACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGtcct                                                                                                                   <  7:230124/140‑1 (MQ=255)
                                cACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGtcct                                                                                                                   >  8:230124/1‑140 (MQ=255)
                                cACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGc                                                                                                           >  5:116573/1‑148 (MQ=255)
                                 aCTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCg                                                                                                          >  4:316182/1‑148 (MQ=255)
                                   tCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGcc                                                                                                                          >  4:211531/1‑130 (MQ=255)
                                   tCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGcc                                                                                                                          <  3:211531/130‑1 (MQ=255)
                                    ccAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAAc                                                                                                      <  3:316182/149‑1 (MQ=255)
                                     cAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAAcc                                                                                                     >  3:149041/1‑149 (MQ=255)
                                     cAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAAc                                                                                                      >  5:96408/1‑148 (MQ=255)
                                        ccTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCa                                                                                                  <  7:163060/149‑1 (MQ=255)
                                           gTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACgg                                                                                               >  6:358259/1‑149 (MQ=255)
                                                tgCGTTCAGGCCAGCGGGATGATCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATTAATCCACACTTAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTccc                                                                                           <  5:230480/147‑1 (MQ=255)
                                                 gCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGAGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCAt                                                                                         >  1:160028/1‑149 (MQ=255)
                                                   gTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATcc                                                                                       >  1:311649/1‑149 (MQ=255)
                                                    ttCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCt                                                                                      >  4:234848/1‑149 (MQ=255)
                                                      cAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCtt                                                                                     <  2:311649/148‑1 (MQ=255)
                                                       aGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCtt                                                                                                                                    <  2:125486/100‑1 (MQ=255)
                                                       aGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCtt                                                                                                                                    >  1:125486/1‑100 (MQ=255)
                                                         gCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATc                                                                                        <  2:255920/142‑1 (MQ=255)
                                                         gCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATc                                                                                        >  1:255920/1‑142 (MQ=255)
                                                         gCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGgt                                                                                 <  3:234848/149‑1 (MQ=255)
                                                           cACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCa                                                                                                  <  4:184246/130‑1 (MQ=255)
                                                           cACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCa                                                                                                  >  3:184246/1‑130 (MQ=255)
                                                                ggATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACAcc                                                                          <  8:13990/149‑1 (MQ=255)
                                                                           tCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CACCCCAggg                                                              <  4:149041/149‑2 (MQ=255)
                                                                              gCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGcccc                                                           <  6:116573/149‑1 (MQ=255)
                                                                                    gccAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAg                                                        <  1:30357/146‑1 (MQ=255)
                                                                                    gccAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAg                                                        >  2:30357/1‑146 (MQ=255)
                                                                                       aGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCa                                                   <  6:363826/148‑1 (MQ=255)
                                                                                       aGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCACCAGGTc                                                     >  5:363826/1‑146 (MQ=255)
                                                                                        gggCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAgg                                                 <  2:160028/149‑1 (MQ=255)
                                                                                             gATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGcacgt                                            >  2:96435/1‑149 (MQ=255)
                                                                                             gATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGcacgt                                            <  6:33893/149‑1 (MQ=255)
                                                                                                      tCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACtt                                   <  3:310942/149‑1 (MQ=255)
                                                                                                        aGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTgg                                 <  5:358259/149‑1 (MQ=255)
                                                                                                          ccGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGcc                                                                                                                          <  4:215726/59‑1 (MQ=255)
                                                                                                          ccGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGcc                                                                                                                          >  3:215726/1‑59 (MQ=255)
                                                                                                            gCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGggctg                             >  2:281753/1‑149 (MQ=255)
                                                                                                              tGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGggctggc                           >  4:95819/1‑149 (MQ=255)
                                                                                                               ggCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGggctggct                          >  5:228972/1‑149 (MQ=255)
                                                                                                                  ggATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGt                        >  7:291620/1‑148 (MQ=255)
                                                                                                                          ccACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGAACGTCACGTACTTGGGCTGGCTGTAAGCCGTTg               >  8:200873/1‑149 (MQ=255)
                                                                                                                           cacaCTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCGTCGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGtt                <  1:96435/148‑1 (MQ=255)
                                                                                                                            acacTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGTTGGt             <  1:281753/149‑1 (MQ=255)
                                                                                                                             cacTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGTTGGTg            <  8:291620/149‑1 (MQ=255)
                                                                                                                                    ttGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGTTGGTGCTACGc      >  4:167132/1‑148 (MQ=255)
                                                                                                                                       aTCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCGTTCGGTGTAGAGCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGTTGGTGCTACGCCaa   <  6:96408/148‑1 (MQ=255)
                                                                                                                                       aTCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGTTGGTGCTACGCCaa   <  3:95819/148‑1 (MQ=255)
                                                                                                                                           ggCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGTTGGTGCTACGCCAAg  <  7:200666/145‑1 (MQ=255)
                                                                                                                                           ggCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGTTGGTGCTACGCCAAg  >  8:200666/1‑145 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
GTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGATCAGCTGCCGCCAGGGCCGATGCCCGGTCAGCCGCTGGCGGATAAATCCACACTGAATTGATCTGGCGCTTCAGCGCCTTGGCTTCGGCCTTGTCCTCCGGTGGCGAAACCTCACGGTCCCATCCTTCGGTGTACACCG‑CGCCCCAGGCCCCCAGGTCCAGGCACGTCACGTACTTGGGCTGGCTGTAAGCCGTTGGTGCTACGCCAAG  >  NC_002947/3268157‑3268440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑