Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,273,641 G→C 5.6% G106A (GGC→GCC)  PP_2871 → class II aldolase/adducin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,273,6410GC 5.6% 227.0 / 2.4 70G106A (GGC→GCC) PP_2871class II aldolase/adducin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (34/32);  new base C (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GCGTCCAGCCTGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAG  >  NC_002947/3273493‑3273789
                                                                                                                                                     |                                                                                                                                                    
gCGTCCAGCCTGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAgg                                                                                                                                                      <  1:236762/149‑1 (MQ=255)
     cAGCCTGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGCCTgcg                                                                                                                                                 >  1:67585/1‑149 (MQ=255)
             tGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGcc                                                                                                                                         <  3:165395/149‑1 (MQ=255)
             tGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACA‑CCGCGCAGCCTGCGCgggtgtgg                                                                                                                                        <  3:155176/149‑7 (MQ=255)
               aaGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGcgcg                                                                                                                                               <  5:39698/141‑1 (MQ=255)
               aaGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGcgcg                                                                                                                                               >  6:39698/1‑141 (MQ=255)
               aaGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGCCTGCGCgggtgtgg                                                                                                                                        <  2:67585/148‑7 (MQ=255)
                 gATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCAt                                                                                                                                     <  3:144469/149‑1 (MQ=255)
                  aTCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCAtt                                                                                                                                    >  4:5826/1‑149 (MQ=255)
                   tCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTg                                                                                                                                   >  1:235217/1‑149 (MQ=255)
                      aCCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGaa                                                                                                                                >  5:36100/1‑149 (MQ=255)
                      aCCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGaa                                                                                                                                >  7:275270/1‑149 (MQ=255)
                           cAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAAtgtg                                                                                                                            <  1:254706/148‑1 (MQ=255)
                           cAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAAtgtg                                                                                                                            <  3:5826/148‑1 (MQ=255)
                             ggggCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGcc                                                                                                                         >  6:230819/1‑149 (MQ=255)
                              gggCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAAt                                                                                                                               >  3:297102/1‑142 (MQ=255)
                              gggCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAAt                                                                                                                               <  4:297102/142‑1 (MQ=255)
                              gggCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCct                                                                                                                        >  1:105780/1‑149 (MQ=255)
                                           gggCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCTTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGTTCaa                                                                                                            >  1:252568/1‑149 (MQ=255)
                                              cTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAg                                                                                                        <  6:55898/149‑1 (MQ=255)
                                                 ccTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGAt                                                                                                      <  2:235217/148‑1 (MQ=255)
                                                  cTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGt                                                                                                    >  2:3819/1‑149 (MQ=255)
                                                  cTGGCATCCGGTCAACCCGGCCGGGTTGGTCATTCACA‑GTGCAATTCACGCTGCCCGCGAGTATGCCCGGTGCGTGCTGCACACCCACACCCGCGCTGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGt                                                                                                    <  2:76979/149‑1 (MQ=255)
                                                   tGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAAc                                                                                                           >  3:87698/1‑141 (MQ=255)
                                                   tGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAAc                                                                                                           <  4:87698/141‑1 (MQ=255)
                                                   tGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTc                                                                                                   >  2:249813/1‑149 (MQ=255)
                                                   tGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTc                                                                                                   >  6:131319/1‑149 (MQ=255)
                                                    gTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGACCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTc                                                                                                   >  3:56992/1‑148 (MQ=255)
                                                       aTCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATg                                                                                               >  1:311262/1‑149 (MQ=255)
                                                         ccGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATg                                                                                               >  8:308200/1‑147 (MQ=255)
                                                          cGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCGGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAg                                                                                            <  2:311262/149‑1 (MQ=255)
                                                            gTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGtt                                                                                          >  6:176255/1‑149 (MQ=255)
                                                               aaCCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTa                                                                                       >  2:272322/1‑149 (MQ=255)
                                                                aCCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCTGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTAc                                                                                      <  6:36100/149‑1 (MQ=255)
                                                                aCCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTAc                                                                                      <  1:3819/149‑1 (MQ=255)
                                                                aCCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTAc                                                                                      <  5:230819/149‑1 (MQ=255)
                                                                  ccggccggGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACgg                                                                                    >  5:158307/1‑149 (MQ=255)
                                                                        gggTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAAtgt                                                                              <  2:252568/149‑1 (MQ=255)
                                                                          gTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTgg                                                                            <  5:176255/149‑1 (MQ=255)
                                                                              gTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTa                                                                        >  4:47331/1‑149 (MQ=255)
                                                                                      ca‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTAc                                                                                      >  2:8306/1‑127 (MQ=255)
                                                                                      ca‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTAc                                                                                      <  1:8306/127‑1 (MQ=255)
                                                                                            ccATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGa                                                            <  1:249813/148‑1 (MQ=255)
                                                                                            ccATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGa                                                            <  5:131319/148‑1 (MQ=255)
                                                                                            ccATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAg                                                           <  2:105780/149‑1 (MQ=255)
                                                                                              aTTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAggg                                                         >  8:309124/1‑149 (MQ=255)
                                                                                                        cccGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTg                                               <  7:308200/149‑1 (MQ=255)
                                                                                                               ggATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGACCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATgg                                        <  4:56992/149‑1 (MQ=255)
                                                                                                                     ccGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAgg                                                          >  1:276785/1‑125 (MQ=255)
                                                                                                                     ccGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAgg                                                          <  2:276785/125‑1 (MQ=255)
                                                                                                                      cGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACa                                 <  1:272322/149‑1 (MQ=255)
                                                                                                                           cgtgcTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAAc                            <  8:275270/149‑1 (MQ=255)
                                                                                                                             tgctgcACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACgg                          <  7:309124/149‑1 (MQ=255)
                                                                                                                                 gcACCCCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCAGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGCGCGTAGCGCTGGACATGCATCAACAGCAACGGCtgg                      >  2:6492/1‑149 (MQ=255)
                                                                                                                                 gcACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCtgg                      >  8:253634/1‑149 (MQ=255)
                                                                                                                                   acacccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCa                                                                                  <  6:146978/87‑1 (MQ=255)
                                                                                                                                   acacccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCa                                                                                  >  5:146978/1‑87 (MQ=255)
                                                                                                                                   acacccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCaggtg                    >  1:291880/1‑149 (MQ=255)
                                                                                                                                     acccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACgg                          <  6:313783/141‑1 (MQ=255)
                                                                                                                                     acccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACgg                          >  5:313783/1‑141 (MQ=255)
                                                                                                                                      cccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGAt                                      <  5:44633/128‑1 (MQ=255)
                                                                                                                                      cccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGAt                                      >  6:44633/1‑128 (MQ=255)
                                                                                                                                       ccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCgcgc                                                 >  5:254813/1‑116 (MQ=255)
                                                                                                                                       ccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCgcgc                                                 <  6:254813/116‑1 (MQ=255)
                                                                                                                                       ccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACTGCTGGTGGCCg                >  7:291771/1‑149 (MQ=255)
                                                                                                                                       ccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATAGAt                                      <  3:214308/127‑1 (MQ=255)
                                                                                                                                       ccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATAGAt                                      >  4:214308/1‑127 (MQ=255)
                                                                                                                                        cacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCACCGGCTTGTTGcc                 >  1:181857/1‑147 (MQ=255)
                                                                                                                                            accGCGCAGCCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTACATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTg           >  6:20983/2‑149 (MQ=255)
                                                                                                                                             ccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTgg          >  7:26658/1‑149 (MQ=255)
                                                                                                                                             ccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTgg          <  8:26658/149‑1 (MQ=255)
                                                                                                                                                     gCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAg  >  8:289958/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGTCCAGCCTGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACA‑GTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAG  >  NC_002947/3273493‑3273789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑