Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,281,140 C→G 6.8% A141G (GCT→GGT) ‡ PP_2881 → short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,281,1400CG 6.8% 191.7 / 3.9 59A141G (GCT→GGT) ‡PP_2881short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (27/28);  new base G (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CGTGCAGCATTCTCGAGGAAACCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCATGTGGTGGTGGACGGTGCCATCGACA  >  NC_002947/3281002‑3281286
                                                                                                                                          |                                                                                                                                                  
cGTGCAGCATTCTCGAGGAAACCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCTGTAGCGCCGGt                                                                                                                                          >  5:1320/1‑149 (MQ=255)
 gTGCAGCATTCTCGAGGAAACCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCTGTAGCGCCGGtg                                                                                                                                         <  6:1320/149‑2 (MQ=255)
   gcagcaTTCTCGAGGAAACCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTAcc                                                                                                                                       >  7:150161/1‑149 (MQ=255)
               aGGAAACCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACTCGCGGCGCAGcc                                                                                                                           <  8:52880/149‑1 (MQ=255)
               aGGAAACCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGcc                                                                                                                           <  6:125636/149‑1 (MQ=255)
                ggAAACCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGc                                                                                                                                              >  8:59779/1‑129 (MQ=255)
                ggAAACCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGc                                                                                                                                              <  7:59779/129‑1 (MQ=255)
                   aaCCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGtt                                                                                                                       >  3:95292/1‑149 (MQ=255)
                      cccACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGACCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTg                                                                                                                    >  2:7910/1‑149 (MQ=255)
                        cACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTgcc                                                                                                                  >  8:126626/1‑149 (MQ=255)
                           gCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTgc                                                                                                                   <  5:14441/145‑1 (MQ=255)
                           gCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTgc                                                                                                                   >  6:14441/1‑145 (MQ=255)
                             aaGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCTGTAGCGCCGGtgaacag                                                                                                                                    >  5:264656/1‑120 (MQ=255)
                             aaGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCTGTAGCGCCGGtgaacag                                                                                                                                    <  6:264656/126‑7 (MQ=255)
                             aaGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCtt                                                                                                             >  7:132533/1‑149 (MQ=255)
                             aaGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCtt                                                                                                             >  3:200634/1‑149 (MQ=255)
                              aGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTc                                                                                                            >  7:177888/1‑149 (MQ=255)
                                   ttCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCgg                                                                                                       >  2:5021/1‑149 (MQ=255)
                                   ttCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCgg                                                                                                       >  8:57560/1‑149 (MQ=255)
                                     cAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGtttt                                                                                                                     <  6:281651/133‑1 (MQ=255)
                                     cAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGtttt                                                                                                                     >  5:281651/1‑133 (MQ=255)
                                     cAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCggg                                                                                                      >  4:65588/1‑148 (MQ=255)
                                     cAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCggg                                                                                                      <  7:126626/148‑1 (MQ=255)
                                     cAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCggg                                                                                                      <  3:65588/148‑1 (MQ=255)
                                     cAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCgg                                                                                                       <  6:319955/147‑1 (MQ=255)
                                        gATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGgcgc                                                                                                                                                    <  6:254186/99‑1 (MQ=255)
                                        gATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGgcgc                                                                                                                                                    >  5:254186/1‑99 (MQ=255)
                                          tCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAg                                                                                                >  6:76223/1‑149 (MQ=255)
                                             gggAAATGGCCTGCTTTGCCGGGTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCAt                                                                                             <  7:293213/149‑1 (MQ=255)
                                                  aTGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCtt                                                                                        <  8:150161/149‑1 (MQ=255)
                                                      ccTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGt                                                                                    <  7:57560/149‑1 (MQ=255)
                                                      ccTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGt                                                                                    <  1:5021/149‑1 (MQ=255)
                                                               ccGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGca                                                                           <  5:76223/149‑1 (MQ=255)
                                                                  gTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGca                                                                           >  1:121666/1‑146 (MQ=255)
                                                                   tttCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACaga                                                                       <  8:177888/149‑1 (MQ=255)
                                                                       cTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCAt                                                                   >  3:254095/1‑149 (MQ=255)
                                                                       cTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCAt                                                                   >  6:181161/1‑149 (MQ=255)
                                                                             gcgcAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGc                                                                            <  6:26553/134‑1 (MQ=255)
                                                                             gcgcAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGc                                                                            >  5:26553/1‑134 (MQ=255)
                                                                              cgcAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATg                                                                  <  6:160578/143‑1 (MQ=255)
                                                                              cgcAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATg                                                                  >  5:160578/1‑143 (MQ=255)
                                                                                cAGGCGGTGACCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGa                                                          <  1:7910/149‑1 (MQ=255)
                                                                                     ggTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTgg                                                     <  4:200634/149‑1 (MQ=255)
                                                                                      gTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTggg                                                    <  5:181161/149‑1 (MQ=255)
                                                                                           ccGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCgg                                                                                                       <  6:273987/93‑1 (MQ=255)
                                                                                           ccGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCgg                                                                                                       >  5:273987/1‑93 (MQ=255)
                                                                                           ccGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCAc                                               <  4:95292/149‑1 (MQ=255)
                                                                                           ccGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCAc                                               <  4:254095/149‑1 (MQ=255)
                                                                                                                      cacaATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCATgtggtgg                    >  5:147016/1‑149 (MQ=255)
                                                                                                                       acaATCCTGTTGACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCATgtggtggt                   <  8:132533/149‑1 (MQ=255)
                                                                                                                       acaATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCATgtggtggt                   <  6:147016/149‑1 (MQ=255)
                                                                                                                        caATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCATgtggtggtg                  >  5:278253/1‑149 (MQ=255)
                                                                                                                         aaTCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGc                                                                                      <  4:145574/80‑1 (MQ=255)
                                                                                                                           tCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCATGTGGTGGTGGAc               >  5:343987/1‑149 (MQ=255)
                                                                                                                            ccTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATgg                                                                 <  6:158956/98‑1 (MQ=255)
                                                                                                                            ccTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATgg                                                                 >  5:158956/1‑98 (MQ=255)
                                                                                                                              tGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATcc                                      <  4:213723/123‑1 (MQ=255)
                                                                                                                              tGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATcc                                      >  3:213723/1‑123 (MQ=255)
                                                                                                                                ttCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCAtgt                         >  2:295628/1‑134 (MQ=255)
                                                                                                                                ttCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCAtgt                         <  1:295628/134‑1 (MQ=255)
                                                                                                                                   aCCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCATGTGGTGGTGGACGGTGCCAt       >  1:242057/1‑149 (MQ=255)
                                                                                                                                        cgcTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCATGTGGTGGTGGACGGTGCCATCGaca  >  6:359863/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
CGTGCAGCATTCTCGAGGAAACCCCACGCAAGTACTTCAAGATCTGGGAAATGGCCTGCTTTGCCGGTTTCCTCACCGCGCAGGCGGTGGCCCGGCGCATGGTTCAGCGTGAGCGCGGCACAATCCTGTTCACCGGCGCTACAGCCGGTACCCGCGGCGCAGCCGGTTTTGCCGCCTTCGCCGGGGCCAAGCATGGCTTGCGTGCCCTGGCACAGAGCATGGCGCGCGAGTTGGGGCCACGCAACATCCATGTCGCCCATGTGGTGGTGGACGGTGCCATCGACA  >  NC_002947/3281002‑3281286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑