Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,299,965 A→G 7.5% S728G (AGT→GGT)  pvdQ → acyl‑homoserine lactone acylase PvdQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,299,9650AG 7.5% 147.5 / 2.1 53S728G (AGT→GGT) pvdQacyl‑homoserine lactone acylase PvdQ
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base G (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TACAGCGTGCCGCACGGGCAGGGCAAGCGGCTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCC  >  NC_002947/3299821‑3300112
                                                                                                                                                |                                                                                                                                                   
tACAGCGTGCCGCACGGGCAGGGCAAGCGGCTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCGGTga                                                                                                                                                 >  7:314348/1‑149 (MQ=255)
tACAGCGTGCCGCACGGGCAGGGCAAGCGGCTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCGGTga                                                                                                                                                 >  7:318999/1‑149 (MQ=255)
          cGCACGGGCAGGGCAAGCGGCTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAg                                                                                                                                       <  8:312406/149‑1 (MQ=255)
           gCACGGGCAGGGCAAGCGGCTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAgg                                                                                                                                      >  8:13839/1‑149 (MQ=255)
                          gCGGCTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGc                                                                                                                       <  7:13839/149‑1 (MQ=255)
                                ggtggtGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCGGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTgg                                                                                                                 <  8:318999/149‑1 (MQ=255)
                                 gtggtgAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCGGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTgg                                                                                                                 <  8:314348/148‑1 (MQ=255)
                                 gtggtgAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTg                                                                                                                  <  1:194970/147‑1 (MQ=255)
                                    gtgAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCt                                                                                                             >  7:334727/1‑149 (MQ=255)
                                    gtgAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCt                                                                                                             >  2:114330/1‑149 (MQ=255)
                                     tgAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCta                                                                                                            >  3:182461/1‑148 (MQ=255)
                                     agAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTAGCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGgcgc                                                                                                              <  4:182461/146‑1 (MQ=255)
                                           gCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCg                                                                                                      <  8:334727/149‑1 (MQ=255)
                                           gCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCg                                                                                                      <  6:116669/149‑1 (MQ=255)
                                             aCCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGtt                                                                                                    <  5:94666/149‑1 (MQ=255)
                                              ccAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGttt                                                                                                   <  4:282545/149‑1 (MQ=255)
                                               cAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTa                                                                                                  >  1:9386/1‑149 (MQ=255)
                                                                  aGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAgg                                                                                >  6:50796/1‑148 (MQ=255)
                                                                  aGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGc                                                                               <  2:9386/149‑1 (MQ=255)
                                                                          cGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGgc                                                                                           <  5:345189/129‑1 (MQ=255)
                                                                          cGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGgc                                                                                           >  6:345189/1‑129 (MQ=255)
                                                                           gACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCt                                                                        >  1:22365/1‑147 (MQ=255)
                                                                           gACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGa                                                                      <  6:62946/149‑1 (MQ=255)
                                                                                   gCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCgg                                                               >  8:131128/1‑148 (MQ=255)
                                                                                     cAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGaa                                                            >  3:95019/1‑149 (MQ=255)
                                                                                      aGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAg                                                           >  5:147207/1‑149 (MQ=255)
                                                                                        aaGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGa                                                                                      <  5:21194/120‑1 (MQ=255)
                                                                                        aaGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGa                                                                                      >  6:21194/1‑120 (MQ=255)
                                                                                        aaGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAgtg                                                         <  5:50796/149‑1 (MQ=255)
                                                                                         aGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAgtgg                                                        <  2:22365/149‑1 (MQ=255)
                                                                                         aGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAgtgg                                                        <  6:147207/149‑1 (MQ=255)
                                                                                                   cTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGt                                                >  1:294728/1‑147 (MQ=255)
                                                                                                   cTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGt                                                >  3:21995/1‑147 (MQ=255)
                                                                                                   cTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGa                                              <  7:131128/149‑1 (MQ=255)
                                                                                                       ttGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGa                                                                      <  8:3972/121‑1 (MQ=255)
                                                                                                       ttGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGa                                                                      >  7:3972/1‑121 (MQ=255)
                                                                                                       ttGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCgg                                          <  4:21995/149‑1 (MQ=255)
                                                                                                           gTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCa                                                  >  3:241377/1‑137 (MQ=255)
                                                                                                           gTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCa                                                  <  4:241377/137‑1 (MQ=255)
                                                                                                               tCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCa                                                  <  2:96580/133‑1 (MQ=255)
                                                                                                               tCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCa                                                  >  1:96580/1‑133 (MQ=255)
                                                                                                                 ccAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGa                                                                                      <  6:196912/95‑1 (MQ=255)
                                                                                                                 ccAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGa                                                                                      >  5:196912/1‑95 (MQ=255)
                                                                                                                 ccAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAggggg                                >  4:343405/1‑149 (MQ=255)
                                                                                                                    gTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCgg                             <  3:343405/149‑1 (MQ=255)
                                                                                                                                ggCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTcagccagc                 >  4:279186/1‑149 (MQ=255)
                                                                                                                                 gCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCagccagcc                <  1:114330/149‑1 (MQ=255)
                                                                                                                                     cGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCt              <  3:279186/147‑1 (MQ=255)
                                                                                                                                       gcgcATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAgg          >  6:309371/1‑149 (MQ=255)
                                                                                                                                            tGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGt       >  8:181766/1‑147 (MQ=255)
                                                                                                                                              ccAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGc   <  2:282808/149‑1 (MQ=255)
                                                                                                                                               cAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGcc  >  7:8808/1‑149 (MQ=255)
                                                                                                                                               cAGTGACAAGACCAAGGCCTATGCGGACAAGCAACAGGCGCTGATTCCGTTTACAGAGGCGCAGATCAACGCAGATACTGAGTACCGGGAAGTGGTGATATGTGAGAGGGACAACGGGGCGGTAGTCAGCCAGCCCTGAGGAGTCAgac  >  8:202311/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TACAGCGTGCCGCACGGGCAGGGCAAGCGGCTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGCCCTGAGGCGTCAGCC  >  NC_002947/3299821‑3300112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑