Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,305,308 A→G 6.4% L288S (TTG→TCG)  PP_2906 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,305,3080AG 6.4% 181.8 / 2.3 62L288S (TTG→TCG) PP_2906sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base G (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

GTTGAATGCCGCCATCCTCGGCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATGGGCAATGTTGTCGCACACGCCCTT  >  NC_002947/3305162‑3305453
                                                                                                                                                  |                                                                                                                                                 
gTTGAATGCCGCCATCCTCGGCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAg                                                                                                                                                 >  2:164526/1‑149 (MQ=255)
 ttGAATGCCGCCATCCTCGGCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAgg                                                                                                                                                >  2:230653/1‑149 (MQ=255)
    aaTGCCGCCATCCTCGGCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGGTGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGgtgt                                                                                                                                             <  2:133588/149‑1 (MQ=255)
               ccTCGGCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGcc                                                                                                                                   >  3:284323/1‑148 (MQ=255)
               ccTCGGCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGcc                                                                                                                                   >  4:100088/1‑148 (MQ=255)
                   ggCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGATGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACACGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATg                                                                                                                               <  6:337184/148‑1 (MQ=255)
                   ggCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATgg                                                                                                                              >  4:42408/1‑149 (MQ=255)
                    gCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGc                                                                                                                             >  2:67178/1‑149 (MQ=255)
                      cAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCGAGGTGTCGGTTTCGCCAATGGCCt                                                                                                                           >  7:177200/1‑149 (MQ=255)
                      cAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCGAGGTGTCGGTTTCGCCAATGGCCt                                                                                                                           >  7:178317/1‑149 (MQ=255)
                       aaTGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTg                                                                                                                          <  1:164526/149‑1 (MQ=255)
                           gCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATCGCCTGGTcc                                                                                                                      >  8:286585/1‑149 (MQ=255)
                               gTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAgcg                                                                                                                  >  2:109767/1‑149 (MQ=255)
                                tAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGTTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAgcgc                                                                                                                 >  1:5409/1‑149 (MQ=255)
                                   aaaTCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTc                                                                                                              <  1:109767/149‑1 (MQ=255)
                                             gTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCa                                                                                                    <  3:42408/149‑1 (MQ=255)
                                                 tACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGcc                                                                                                                            <  4:232771/121‑1 (MQ=255)
                                                 tACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGcc                                                                                                                            >  3:232771/1‑121 (MQ=255)
                                                   ccAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCGAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATcc                                                                                              <  8:177200/149‑1 (MQ=255)
                                                   ccAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCGAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATcc                                                                                              <  8:178317/149‑1 (MQ=255)
                                                     agcagcTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGTTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCAc                                                                                            <  2:5409/149‑1 (MQ=255)
                                                     agcagcTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCAc                                                                                            <  1:67178/149‑1 (MQ=255)
                                                                   gTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGc                                                                              <  3:100088/149‑1 (MQ=255)
                                                                      aaGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGaa                                                                           >  8:93883/1‑149 (MQ=255)
                                                                            gATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCa                                                                                             >  3:267218/1‑125 (MQ=255)
                                                                            gATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCa                                                                                             <  4:267218/125‑1 (MQ=255)
                                                                             aTGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTa                                                                    >  6:157293/1‑149 (MQ=255)
                                                                              tGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTa                                                                    >  1:81913/1‑148 (MQ=255)
                                                                              tGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTa                                                                    >  3:207464/1‑148 (MQ=255)
                                                                               gAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTg                                                                                                                          <  6:71911/93‑1 (MQ=255)
                                                                               gAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCg                                                                                                             <  4:255146/106‑1 (MQ=255)
                                                                                aaGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGaa                                                                                                 >  4:344339/1‑117 (MQ=255)
                                                                                aaGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGaa                                                                                                 <  3:344339/117‑1 (MQ=255)
                                                                                  gCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCt                                                               <  5:157293/149‑1 (MQ=255)
                                                                                   cccGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTg                                                              >  5:187146/1‑149 (MQ=255)
                                                                                    ccGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGc                                                                                                                             <  6:289992/85‑1 (MQ=255)
                                                                                    ccGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGc                                                                                                                             >  5:289992/1‑85 (MQ=255)
                                                                                     cgcgcgGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTa                                                                    >  3:210566/1‑141 (MQ=255)
                                                                                     cgcgcgGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTa                                                                    <  4:210566/141‑1 (MQ=255)
                                                                                                 gCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCgg                                                 <  6:187146/148‑1 (MQ=255)
                                                                                                       ttCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCg                                          >  5:82342/1‑149 (MQ=255)
                                                                                                       aaccAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGAGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTACGGCTTGCGCAGAATCACCCTCGCTTTGAGGGCGAATACGTTACAGCTGCGCGCGCAGGCGGGTCAGCg                                          >  5:82347/3‑149 (MQ=255)
                                                                                                          cAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGgt                                        <  1:230653/148‑1 (MQ=255)
                                                                                                                gCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTgcgc                                  <  7:280064/148‑1 (MQ=255)
                                                                                                                     tGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCa                            <  6:82342/149‑1 (MQ=255)
                                                                                                                      tcgccataacCCGCGAAAGCGGGCCATCAAGGTGTCGTTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCa                            <  6:82347/141‑1 (MQ=255)
                                                                                                                       cgcAACAAGCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATg                          <  4:284323/149‑1 (MQ=255)
                                                                                                                        gcAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATgg                         >  5:85484/1‑149 (MQ=255)
                                                                                                                        gcAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATgg                         >  5:90548/1‑149 (MQ=255)
                                                                                                                         caacaaCCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCgg                                                 <  6:351672/124‑1 (MQ=255)
                                                                                                                            caacCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTgcgc                                  >  3:10208/1‑136 (MQ=255)
                                                                                                                            caacCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTgcgc                                  <  4:10208/136‑1 (MQ=255)
                                                                                                                               cccGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACg                                                                       <  2:198330/96‑1 (MQ=255)
                                                                                                                               cccGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACg                                                                       >  1:198330/1‑96 (MQ=255)
                                                                                                                                ccGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATGGGCAAtgtt                 >  5:207912/1‑149 (MQ=255)
                                                                                                                                   cgAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATGGGCAATGTTGTc              <  7:93883/149‑1 (MQ=255)
                                                                                                                                    gAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAg                                >  6:22052/1‑130 (MQ=255)
                                                                                                                                    gAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAg                                <  5:22052/130‑1 (MQ=255)
                                                                                                                                               aTCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATgg                         <  7:215640/126‑1 (MQ=255)
                                                                                                                                               aTCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATgg                         >  8:215640/1‑126 (MQ=255)
                                                                                                                                               aTCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGAGCAGGTCATGGGCAATGTTGTCGCACACGCCCtt  >  3:114823/1‑149 (MQ=255)
                                                                                                                                                tCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTCCGCACAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATGGGCAATGTTGTCGCACACGCCCtt  >  1:306085/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTGAATGCCGCCATCCTCGGCCAATGGCAGGTAAAAATCATGCAGTTCTACCAGCAGCTCATGCGGGTCAAGCTGGATGAAGCCCGCGCGGCGCTGGCGGTCTTCCAGCTCGCTGATGCGCAACAACCCGCGAAAGCGCGCCATCAAGGTGTCGGTTTCGCCAATGGCCTGGTCCAGCGCTTCGGCTTGCGCAGAATCCACGTCGCTTTGCTGGCGAATACGGTACAGCTGGGCGCGCAGGCGGGTCAGCGGTGTGCGCAGGTCATGGGCAATGTTGTCGCACACGCCCTT  >  NC_002947/3305162‑3305453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑