Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,332,008 G→C 8.7% intergenic (‑7/+179) nspC ← / ← PP_2930 carboxynorspermidine/carboxyspermidine decarboxylase/L‑serine dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,332,0080GC 8.7% 139.9 / 5.2 46intergenic (‑7/+179)nspC/PP_2930carboxynorspermidine/carboxyspermidine decarboxylase/L‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base G (24/18);  new base C (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

ACCAGGTGGCGAAGCACTTGAGGGCGAGCAGCGCCTTGGCACCGGAGTGTTCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGCCTAGTAGATCAGGTGCATGTGAC  >  NC_002947/3331875‑3332156
                                                                                                                                     |                                                                                                                                                    
atcAGGTGGCGAAGCACTTGAGGGCGAGCAGCGCCTTGGCACCGGAGTGTTCGCGCACGTATGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAggccg                                                                                                                                       <  5:335635/147‑1 (MQ=255)
          gAAGCACTTGAGGGCGAGCAGCGCCTTGGCACCGGAGTGTTCGCGCACGTAGGCGATCTTCTCCATGTTTCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCaaaaaaa                                                                                                                             <  5:221611/149‑1 (MQ=255)
          gAAGCACTTGAGGGCGAGCAGCGCCTTGGCACCGGAGTGTTCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCaaaaaaa                                                                                                                             <  7:120013/149‑1 (MQ=255)
          gAAGCACTTGAGGGCGAGCAGCGCCTTGGCACCGGAGTGTTCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCaaaaa                                                                                                                               >  5:143191/1‑147 (MQ=255)
               aCTTGAGGGCGAGCAGCGCCTTGGCACCGGAGTGTTCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGaca                                                                                                                        >  2:253738/1‑149 (MQ=255)
                 ttGAGGGCGAGCAGCGCCTTGGCACCGGAGTGTTCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACg                                                                                                                      >  6:324830/1‑149 (MQ=255)
                      ggCGAGCAGCGCCTTGGCACCGGAGTGTTCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATtg                                                                                                                 >  1:165758/1‑149 (MQ=255)
                                            gAGTGTTCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGAcc                                                                                            >  8:270855/1‑148 (MQ=255)
                                               tgtTCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGaaa                                                                                        <  7:270855/149‑1 (MQ=255)
                                                 ttCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTAACGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAgg                                                                                      >  1:82182/1‑149 (MQ=255)
                                                             ggCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCa                                                                          >  5:46056/1‑149 (MQ=255)
                                                                      ctcCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCa                                                                  <  5:324830/148‑1 (MQ=255)
                                                                         cATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGGCACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGt                                                              >  4:166489/1‑149 (MQ=255)
                                                                         cATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATAc                                                                             <  8:10188/134‑1 (MQ=255)
                                                                         cATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATAc                                                                             >  7:10188/1‑134 (MQ=255)
                                                                          aTGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGtt                                                             <  7:58664/149‑1 (MQ=255)
                                                                          aTGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGtt                                                             >  8:58664/1‑149 (MQ=255)
                                                                           tGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGtt                                                             >  7:158813/1‑148 (MQ=255)
                                                                                    gcagcTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTg                                                   >  8:326626/1‑149 (MQ=255)
                                                                                      agcTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCg                                                 <  7:326626/149‑1 (MQ=255)
                                                                                        cTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGAc                                                          >  5:348117/1‑138 (MQ=255)
                                                                                        cTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGAc                                                          <  6:348117/138‑1 (MQ=255)
                                                                                            gTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCt                                           >  5:22254/1‑149 (MQ=255)
                                                                                                  tCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGGCACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCg                                     <  3:166489/149‑1 (MQ=255)
                                                                                                    gATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGt                                   >  2:253547/1‑149 (MQ=255)
                                                                                                    gATGAGGTAATACGGCGTCTTGATCATTTAAAGCCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGt                                   >  4:269216/1‑149 (MQ=255)
                                                                                                    gATGAGGTAATACGGCGTCTTGATCATTTAAAGCCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGt                                   >  4:264405/1‑149 (MQ=255)
                                                                                                        aGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTgc                                <  8:158813/148‑1 (MQ=255)
                                                                                                          gTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACTGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTgcggc                             <  2:165758/149‑1 (MQ=255)
                                                                                                          gTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTgcggc                             >  5:90479/1‑149 (MQ=255)
                                                                                                            aaTACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCa                                                                  <  1:222271/110‑1 (MQ=255)
                                                                                                            aaTACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCa                                                                  >  2:222271/1‑110 (MQ=255)
                                                                                                             aTACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGAATTGTGCCTTCACTTGCCGCAGACCGAAAGCGCGGGATACGCATTTCGTCAGGGTTGAAGCTATTGCGAGTGCTTTTTCGGTTGCGTCGAg                          >  8:308258/1‑149 (MQ=255)
                                                                                                               aCGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGcc                        >  7:33533/1‑149 (MQ=255)
                                                                                                                 ggCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTc                                      <  1:332256/133‑1 (MQ=255)
                                                                                                                 ggCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTc                                      >  2:332256/1‑133 (MQ=255)
                                                                                                                   cGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATa                                                                              >  8:147066/1‑91 (MQ=255)
                                                                                                                    gTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGCCtagta                   <  1:253547/149‑1 (MQ=255)
                                                                                                                       ttGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGCCtag                     >  5:181343/1‑144 (MQ=255)
                                                                                                                        tGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCgg                                    >  5:360500/1‑128 (MQ=255)
                                                                                                                        tGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCgg                                    <  6:360500/128‑1 (MQ=255)
                                                                                                                         gATCATTTAAAGCCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGCCTAGTAGAt                <  3:269216/147‑1 (MQ=255)
                                                                                                                         gATCATTTAAAGCCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGCCTAGTAGAt                <  3:264405/147‑1 (MQ=255)
                                                                                                                           tCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGCCTAGTAGATCAgg            <  6:181343/149‑1 (MQ=255)
                                                                                                                             aTTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGCCTAGTAGATCAGGt           <  6:46056/148‑1 (MQ=255)
                                                                                                                               ttAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTTTGCCTTCACTTGCCGCTGACAGAAAGGGCGGAATACGAATTTCGTCAGGGATGACGATATTTCGAGGCCTTTTTCGGTTGCGTCGAGCCTAGTAGATAAGGtcaa        >  7:59115/1‑146 (MQ=255)
                                                                                                                                    ggCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCAGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTTACGCTATTGCGAGGGCTTGTTCGTTTGCGGAGAGCCTAGTAGATCAGGTGCAGGTGa   >  4:199276/1‑149 (MQ=255)
                                                                                                                                     gCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGCCTAGTAGATCAGGTGCATGTGAc  >  6:217137/1‑149 (MQ=255)
                                                                                                                                     |                                                                                                                                                    
ACCAGGTGGCGAAGCACTTGAGGGCGAGCAGCGCCTTGGCACCGGAGTGTTCGCGCACGTAGGCGATCTTCTCCATGTTGCTGAGCAGCTTGGTTTTATCGATGAGGTAATACGGCGTCTTGATCATTTAAAGGCCCCCGGCCAGGCCGGCCAAAAAAAGGACACGCATTGTGCCTTCACTTGCCGCAGACCGAAAGGGCGGGATACGCATTTCGTCAGGGTTGACGCTATTGCGAGGGCTTTTTCGGTTGCGGCGAGCCTAGTAGATCAGGTGCATGTGAC  >  NC_002947/3331875‑3332156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑