Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,353,153 C→T 7.8% G161D (GGC→GAC)  PP_2948 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,353,1530CT 7.8% 146.1 / 2.4 51G161D (GGC→GAC) PP_2948GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (24/23);  new base T (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

TTTCAGCAATGCGTGAAAATTGAAATAGCAGGGGTCGTCGACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCTTCGAACATCCAGGAGGCAGGTA  >  NC_002947/3353005‑3353294
                                                                                                                                                    |                                                                                                                                             
tttCAGCAATGCGTGAAAATTGAAATAGCAGGGGTCGTCGACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGc                                                                                                                                               >  4:317248/1‑149 (MQ=255)
       aaTGCGTGAAAATTGAAATAGCAGGGGTCGTCGACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAg                                                                                                                                        >  5:155711/1‑149 (MQ=255)
       aaTGCGTGAAAATTGAAATAGCAGGGGTCGTCGACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAg                                                                                                                                        >  2:146335/1‑149 (MQ=255)
               aaaaTTGAAATAGCAGGGGTCGTCGACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGt                                                                                                                                 <  1:35760/148‑1 (MQ=255)
                 aaTTGAAATAGCAGGGGTCGTCGACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGAc                                                                                                                              <  4:67222/149‑1 (MQ=255)
                   ttGAAATAGCAGTGGTCGTCGACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTAAAGAGGGGCTTCGATACCGATGCCTGTCAGCTTCCGTGAc                                                                                                                              >  3:50070/1‑147 (MQ=255)
                         tAGCAGGGGTCGTCGACCAGCATAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGAATCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTg                                                                                                                      >  7:220919/1‑149 (MQ=255)
                           gCAGGGGTCGTCGACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGcc                                                                                                                    >  5:193572/1‑149 (MQ=255)
                                    gtcgACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCgg                                                                                                             >  7:29322/1‑147 (MQ=255)
                                        aCCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATg                                                                                                       >  5:298126/1‑149 (MQ=255)
                                          cAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGcc                                                                                                     >  1:21485/1‑149 (MQ=255)
                                                  tgtCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAgtggt                                                                                             <  6:298126/149‑1 (MQ=255)
                                                      ccccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAgg                                                                                         >  1:335526/1‑149 (MQ=255)
                                                      ccccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAg                                                                                          <  2:21485/148‑1 (MQ=255)
                                                      ccccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAg                                                                                          <  6:193572/148‑1 (MQ=255)
                                                       cccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGc                                                                                        <  3:317248/149‑1 (MQ=255)
                                                       cccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGc                                                                                        >  2:153830/1‑149 (MQ=255)
                                                       cccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGc                                                                                        <  1:146335/149‑1 (MQ=255)
                                                       cccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGAAGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGc                                                                                        <  6:155711/149‑1 (MQ=255)
                                                        ccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCACGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCg                                                                                       >  8:242826/1‑149 (MQ=255)
                                                        ccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAACATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAgtggtg                                                                                            <  8:1801/144‑1 (MQ=255)
                                                        ccGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAACATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAgtggtg                                                                                            >  7:1801/1‑144 (MQ=255)
                                                               cagcagAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGAc                                                                                                                              >  8:259679/1‑103 (MQ=255)
                                                                 gcagAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGt                                                                               >  8:158120/1‑148 (MQ=255)
                                                                 gcagAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTc                                                                              <  1:153830/149‑1 (MQ=255)
                                                                    gAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGt                                                                           >  4:70161/1‑149 (MQ=255)
                                                                     aaaaCGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGCGCGGCAGATGCGGAAGTGGGGGGGGGTACTGCGTCTGtt                                                                          >  4:199665/1‑149 (MQ=255)
                                                                                    gTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGTCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGc                                                                                        >  5:198676/1‑120 (MQ=255)
                                                                                    gTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGTCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGc                                                                                        <  6:198676/120‑1 (MQ=255)
                                                                                        aTGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAgtggt                                                                                             <  8:92287/111‑1 (MQ=255)
                                                                                        aTGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAgtggt                                                                                             >  7:92287/1‑111 (MQ=255)
                                                                                                  tGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTCGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCg                                              >  3:150320/1‑148 (MQ=255)
                                                                                                    ccGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAATAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGaa                                            <  8:220919/148‑1 (MQ=255)
                                                                                                     cGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGt                                          <  7:158120/149‑1 (MQ=255)
                                                                                                            agggAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCttt                                     <  3:199665/144‑1 (MQ=255)
                                                                                                            aGTGAGCACGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCttt                                     <  7:242826/147‑1 (MQ=255)
                                                                                                             gTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTgcg                                  >  7:171456/1‑149 (MQ=255)
                                                                                                              tcaGCATGATCTGTTGAAGATGGGCTTCGATCCCGATGCCTGTCAGCTGCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTgcgc                                 <  5:60414/147‑1 (MQ=255)
                                                                                                              tGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTgcg                                  >  3:72188/1‑148 (MQ=255)
                                                                                                                aGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCaa                               <  3:70161/149‑1 (MQ=255)
                                                                                                                     gATCTGTTCAAGAGGGGCTTCGATCCCGATGTCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCgg                          >  4:144705/1‑149 (MQ=255)
                                                                                                                           ttCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCtt                       <  8:171456/146‑1 (MQ=255)
                                                                                                                           ttCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCt                        <  8:29322/145‑1 (MQ=255)
                                                                                                                           ttCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCTTc                      <  2:335526/147‑1 (MQ=255)
                                                                                                                                   gggCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCtt                                      >  2:197086/1‑123 (MQ=255)
                                                                                                                                   gggCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTCTCGGAACGGGCAACGGTGCGAAGTGCtt                                      <  1:197086/123‑1 (MQ=255)
                                                                                                                                     gCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCTTCGAACATCCag            >  3:111427/1‑147 (MQ=255)
                                                                                                                                           aTCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCTTCGAACATCCagga          <  6:275609/143‑1 (MQ=255)
                                                                                                                                           aTCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCTTCGAACATCCagga          >  5:275609/1‑143 (MQ=255)
                                                                                                                                              ccGATGTCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCTTCGAACATCCAGGAGGCAGGTa  <  3:144705/148‑1 (MQ=255)
                                                                                                                                               cGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCTTCGa                    <  3:269553/129‑1 (MQ=255)
                                                                                                                                               cGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCTTCGa                    >  4:269553/1‑129 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
TTTCAGCAATGCGTGAAAATTGAAATAGCAGGGGTCGTCGACCAGCACAGTGTCCCCCGGCTCCAGCAGAAAACGGCAGATCAGGTCGATGGCGTGGGTGCCGGACTCAGTGAGCATGATCTGTTCAAGAGGGGCTTCGATCCCGATGCCTGTCAGCTTCCGTGACAAGAATTGCCTGAGCGGCAGATGCCCAAGTGGTGAGGCGTACTCCGTCAGTTTCGACGTGTCGGAACGGGCAACGGTGCGAAGTGCTTTGCGCAAGCCGGCTTCGAACATCCAGGAGGCAGGTA  >  NC_002947/3353005‑3353294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑