| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,381,973 | T→C | 10.2% | Y207H (TAC→CAC) | PP_2983 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,381,973 | 0 | T | C | 10.2% | 99.9 / 3.5 | 39 | Y207H (TAC→CAC) | PP_2983 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (18/17); new base C (2/2); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GATCAATCTGATATCGAACAGCTCATCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTTGTGGAGCTCGCCAAGCAGTTGTGGTACGAAGAGCGC > NC_002947/3381832‑3382117 | gATCAATCTGATATCGAACAGCTCATCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTg > 4:159670/1‑147 (MQ=255) atatCGAACAGCTCATCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGc < 2:14157/147‑1 (MQ=255) tatCGAACAGCTCATCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGc > 5:58789/1‑146 (MQ=255) tatCGAACAGCTCATCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCga < 3:159670/148‑1 (MQ=255) atCGAACAGCTCATCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGCACCTGGAGGATAGGc > 3:107331/1‑145 (MQ=255) atCGAACAGCTCATCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGCACCTGGAGGATAGGc > 3:112200/1‑145 (MQ=255) aTCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCt < 4:299844/147‑1 (MQ=255) aTCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCt > 3:299844/1‑147 (MQ=255) aTCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTg > 2:125245/1‑148 (MQ=255) cGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCt > 6:4654/1‑139 (MQ=255) cGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCt < 5:4654/139‑1 (MQ=255) taacaaacTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGga < 7:259638/142‑1 (MQ=255) gTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGgaga > 5:164139/1‑149 (MQ=255) cTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCa < 1:125245/149‑1 (MQ=255) tGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCAc > 3:330497/1‑149 (MQ=255) gagGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGATACTGTCACCCagcagc < 4:179790/149‑1 (MQ=255) cAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCt > 2:285259/1‑149 (MQ=255) aaTCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCt > 6:300571/1‑148 (MQ=255) aTTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGcc > 7:91236/1‑148 (MQ=255) tGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGAAGCGCCTTAGCCAAGt < 6:58789/148‑1 (MQ=255) tGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGCACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTc < 4:112200/149‑1 (MQ=255) tGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGCACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTc < 4:107331/149‑1 (MQ=255) aaGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTgg > 5:273631/1‑91 (MQ=255) aaGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTgg < 6:273631/91‑1 (MQ=255) aaGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCtt < 6:164139/149‑1 (MQ=255) gTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTtgt < 4:330497/149‑1 (MQ=255) gcaatgcaaGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTtgtg > 3:285568/1‑148 (MQ=255) caatgcaaGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCagcagc > 2:331291/1‑115 (MQ=255) caaGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTcc > 5:130534/1‑130 (MQ=255) caaGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTcc < 6:130534/130‑1 (MQ=255) tAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTTGTGGAGCTCGCCAAGCAGtt > 5:153207/1‑149 (MQ=255) aTTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGa < 8:20581/118‑1 (MQ=255) aTTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGa > 7:20581/1‑118 (MQ=255) gTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTTGTGGAGCTCGCCAAGCAGTTGTGGTAc < 6:153207/148‑1 (MQ=255) tCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTTGTGGAGCTCGCCAAGCAGTTGTGGTACg > 7:257687/1‑148 (MQ=255) aGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTtgtg < 1:276350/119‑1 (MQ=255) aGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTtgtg > 2:276350/1‑119 (MQ=255) cGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTTGTGGAGCTCGCCAAGCAGTTGTGGTACGAAGAgcg < 8:257687/149‑1 (MQ=255) gAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTTGTGGAGCTCGCCAAGCAGTTGTGGTACGAAGAgcgc < 1:285259/149‑1 (MQ=255) | GATCAATCTGATATCGAACAGCTCATCGAGTGCGGCCGTGTCGATGTCGGCCAGTTTGTTACACTGGCAACAGAGGCAATCGATTATGCCGTGGGCAACCGAAGTGCAATGCAAGGCTGCCTAAAATTGGTCACAGCGAAGTACCTGGAGGATAGGCGAGATGCCACGCCTGGATCGTAAACAAAGCTTTGGCGCGCTCCTGGAGACTGTCACCCAGCAGCGCCTTAGCCAAGTTGCGTCCGATGCTCTTGTGGAGCTCGCCAAGCAGTTGTGGTACGAAGAGCGC > NC_002947/3381832‑3382117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |