Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,404,918 G→A 6.2% C90Y (TGC→TAC)  PP_3016 → lipopolysaccharide core biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,404,9180GA 6.2% 193.5 / 2.5 64C90Y (TGC→TAC) PP_3016lipopolysaccharide core biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (32/28);  new base A (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TGATCCTTGCCTCCGGCGCTTCTGCCAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGCCGAGCCTGCTGGAC  >  NC_002947/3404771‑3405063
                                                                                                                                                   |                                                                                                                                                 
tgATCCTTGCCTCCGGCGCTTCTGCCAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGc                                                                                                                                                  >  7:196656/1‑149 (MQ=255)
        gCCTCCGGCGCTTCTGCCAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAAc                                                                                                                                           <  4:79570/148‑1 (MQ=255)
        gCCTCCGGCGCTTCTGCCAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACa                                                                                                                                          >  6:157558/1‑149 (MQ=255)
              ggCGCTTCTGCCAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGc                                                                                                                                    <  6:274102/149‑1 (MQ=255)
                      tGCCAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCg                                                                                                                            >  6:28163/1‑149 (MQ=255)
                        ccAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTc                                                                                                                             <  6:7912/146‑1 (MQ=255)
                        ccAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGcc                                                                                                                          >  8:285461/1‑149 (MQ=255)
                        ccAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGc                                                                                                                           <  3:267245/148‑1 (MQ=255)
                         cAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCa                                                                                                                         <  5:28163/149‑1 (MQ=255)
                          aaaCATTTTCCGCTGGGCGAGTTTGCTCACTTGACCATCATCGCCATGAACTGGTCGCTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACAGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCAc                                                                                                                        >  6:110746/1‑149 (MQ=255)
                          aaaCATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCAc                                                                                                                        >  4:38055/1‑149 (MQ=255)
                                         ggCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACa                                                                                                          <  7:285461/148‑1 (MQ=255)
                                         ggCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACa                                                                                                          <  3:38055/148‑1 (MQ=255)
                                             aGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGaa                                                                                                     <  5:157558/149‑1 (MQ=255)
                                                    gcACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTgg                                                                                              >  3:20725/1‑149 (MQ=255)
                                                           cccATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCtgtg                                                                                       >  3:31601/1‑149 (MQ=255)
                                                           cccATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCtgtg                                                                                       <  8:196656/149‑1 (MQ=255)
                                                            ccATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTg                                                                                                                                <  6:269192/107‑1 (MQ=255)
                                                            ccATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTg                                                                                                                                >  5:269192/1‑107 (MQ=255)
                                                               tcatcGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAAc                                                                                                    <  2:191709/132‑1 (MQ=255)
                                                               tcatcGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACACTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCt                                                                                   >  3:108159/1‑149 (MQ=255)
                                                                catcGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCtgt                                                                                        <  1:47468/143‑1 (MQ=255)
                                                                catcGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCtgt                                                                                        >  2:47468/1‑143 (MQ=255)
                                                                       aTGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTa                                                                           >  8:148283/1‑149 (MQ=255)
                                                                       aTGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTa                                                                           >  6:88590/1‑149 (MQ=255)
                                                                           aCGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATgcg                                                                       >  2:187579/1‑149 (MQ=255)
                                                                                  ggtcgCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCtgt                                                                                        <  1:234859/125‑1 (MQ=255)
                                                                                  ggtcgCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCtgt                                                                                        >  2:234859/1‑125 (MQ=255)
                                                                                   gtcgCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGa                                                               >  8:297354/1‑149 (MQ=255)
                                                                                    tcgCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGAt                                                              <  7:148283/149‑1 (MQ=255)
                                                                                    tcgCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGAt                                                              <  8:234035/149‑1 (MQ=255)
                                                                                    tcgCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGAt                                                              >  7:234035/1‑149 (MQ=255)
                                                                                    tcgCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGAt                                                              <  5:88590/149‑1 (MQ=255)
                                                                                      gCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGAtgt                                                            >  6:168008/1‑149 (MQ=255)
                                                                                        cATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGc                                                          >  4:272517/1‑149 (MQ=255)
                                                                                           gACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAg                                                                                >  7:114805/1‑124 (MQ=255)
                                                                                           gACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAg                                                                                <  8:114805/124‑1 (MQ=255)
                                                                                            aCCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGc                                                      >  4:182353/1‑149 (MQ=255)
                                                                                                      acGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAg                                            >  2:268110/1‑149 (MQ=255)
                                                                                                              aaaCCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATg                                     <  1:187579/148‑1 (MQ=255)
                                                                                                               aaCCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGcc                                   >  6:164011/1‑149 (MQ=255)
                                                                                                               aaCCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGcc                                   <  4:31601/149‑1 (MQ=255)
                                                                                                                  ccTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTg                                >  1:335454/1‑149 (MQ=255)
                                                                                                                  ccTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTg                                >  1:330175/1‑149 (MQ=255)
                                                                                                                       ttctACGTGTGCACCGACAAAAGCTTTTACCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGcaca                            >  8:324072/1‑148 (MQ=255)
                                                                                                                       ttctACGTGTGCACCGACAAAAGCTTTTACCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGcaca                            >  8:328642/1‑148 (MQ=255)
                                                                                                                         ctACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGAt                                                              >  6:348808/1‑112 (MQ=255)
                                                                                                                         ctACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGAt                                                              <  5:348808/112‑1 (MQ=255)
                                                                                                                            cGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGcc                       <  3:182353/148‑1 (MQ=255)
                                                                                                                            cGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGc                        <  5:168008/147‑1 (MQ=255)
                                                                                                                            cGTGTGCACCGACAAAAGCTTTTACCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGc                        <  7:328642/147‑1 (MQ=255)
                                                                                                                            cGTGTGCACCGACAAAAGCTTTTACCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGc                        <  7:324072/147‑1 (MQ=255)
                                                                                                                             gtgtGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGa                     <  7:297354/149‑1 (MQ=255)
                                                                                                                                  cACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGc                <  4:108159/149‑1 (MQ=255)
                                                                                                                                   aCCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGcc               >  3:213998/1‑149 (MQ=255)
                                                                                                                                   aCCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGcc               >  3:139451/1‑149 (MQ=255)
                                                                                                                                       ccacaaGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGCCGAGc           <  4:295996/145‑1 (MQ=255)
                                                                                                                                         aaaaGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACaaa                          >  6:253506/1‑132 (MQ=255)
                                                                                                                                         aaaaGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACaaa                          <  5:253506/132‑1 (MQ=255)
                                                                                                                                         aaaaGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGCCGAGCct         >  2:7568/1‑149 (MQ=255)
                                                                                                                                         aaaaGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGCCGAGCct         >  4:61872/1‑149 (MQ=255)
                                                                                                                                          aaaGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGCCGAGCctg        >  7:242397/1‑149 (MQ=255)
                                                                                                                                            aGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGCCGAGCctgct      <  4:20725/149‑1 (MQ=255)
                                                                                                                                                 ttGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGCCGAGCCTGCTGGAc  <  1:268110/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGATCCTTGCCTCCGGCGCTTCTGCCAAACATTTTCCGCTGGGCGAGTTTGCGCACTTGCCCATCATCGCCATGAACGGGTCGGTCGCCATGACCGCCGAACACGGCATCAAACCCTTCTTCTACGTGTGCACCGACAAAAGCTTTTGCCAGCAACAGCCGGCCTTGTTCGCCACTGCCGTGCGCGACAGCGAACGCCTGGCCCTGTGGCCTGAGCAGTATGCGCGTGCCGATGTGCCAGCCCACACCCAGTGTTATGCCTTGCACAAAGCCGACATGCCGAGCCTGCTGGAC  >  NC_002947/3404771‑3405063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑