Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,426,492 C→G 10.0% D147E (GAC→GAG) ‡ PP_3042 → terminase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,426,4920CG10.0% 171.3 / 10.4 60D147E (GAC→GAG) ‡PP_3042terminase large subunit
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base G (3/3);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

TGGCTCCGGCCAACATCTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCAAGCT  >  NC_002947/3426344‑3426638
                                                                                                                                                    |                                                                                                                                                  
tGGCTCCGGCCAACATCTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCCTGGac                                                                                                                                                    <  7:320835/149‑1 (MQ=255)
            aCATCTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATTCCTTGGTGTCCAAGGTAtta                                                                                                                                        >  4:271806/1‑148 (MQ=255)
             cATCTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGAAATACCTTGGACACCAAGGAATTc                                                                                                                                        >  7:215114/1‑148 (MQ=255)
                cTTGACGCTACTGCCCACCGGTGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAAtt                                                                                                                                         <  2:225323/144‑1 (MQ=255)
                cTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAAtt                                                                                                                                         >  1:225323/1‑144 (MQ=255)
                   gACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATTCCTTGGTGTCCAAGGTAttacggg                                                                                                                                    <  3:271806/146‑6 (MQ=255)
                   gACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGTCACCAAGGAATTCGAtg                                                                                                                                    <  5:242871/146‑1 (MQ=255)
                   gACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGAtg                                                                                                                                    <  7:334248/146‑1 (MQ=255)
                      gCTACTGCCGAGCTGGGGCTTGGCGAAGCGGGTGTCTTCAGGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCGAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATggtggt                                                                                                                               <  3:218668/148‑1 (MQ=255)
                              ccACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATTCCTTGGTGTCCAAGGTAttacgg                                                                                                                                     <  3:226275/134‑5 (MQ=255)
                              ccACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATTCCTTGGTGTCCAAGGTAttacgg                                                                                                                                     >  4:226275/1‑130 (MQ=255)
                                 ccGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGc                                                                                                                    >  6:206509/1‑148 (MQ=255)
                                         ttGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCa                                                                                                                  >  3:105782/1‑142 (MQ=255)
                                         ttGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCAAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTTTTTGCCa                                                                                                                  <  4:105782/142‑1 (MQ=255)
                                                 gCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGtt                                                                                                                       >  2:156434/1‑129 (MQ=255)
                                                 gCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGtt                                                                                                                       <  1:156434/129‑1 (MQ=255)
                                                    ggTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAg                                                                                                <  8:93696/149‑1 (MQ=255)
                                                          ttCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAAcc                                                                                          >  6:236485/1‑149 (MQ=255)
                                                           tCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAccc                                                                                          >  8:94623/1‑148 (MQ=255)
                                                           tCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAAcc                                                                                          >  6:236968/1‑148 (MQ=255)
                                                             aCGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTa                                                                                        >  4:146998/1‑148 (MQ=255)
                                                                     gCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGAtggtg                                                                                                                                 <  6:32688/99‑1 (MQ=255)
                                                                     gCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCt                                                                                <  3:146998/148‑1 (MQ=255)
                                                                     gCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCAATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCt                                                                                <  6:304667/148‑1 (MQ=255)
                                                                      cAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGt                                                                              >  4:241769/1‑149 (MQ=255)
                                                                       aaGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTc                                                                             >  2:202467/1‑149 (MQ=255)
                                                                          aCCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCa                                                                                                 >  1:170498/1‑126 (MQ=255)
                                                                          aCCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCa                                                                                                 <  2:170498/126‑1 (MQ=255)
                                                                            cATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCaa                                                                                            >  6:296266/1‑129 (MQ=255)
                                                                            cATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCaa                                                                                            <  5:296266/129‑1 (MQ=255)
                                                                             aTTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCg                                                                       <  5:206509/149‑1 (MQ=255)
                                                                                 aTTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTAt                                                                   >  4:134968/1‑149 (MQ=255)
                                                                                  ttCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTAt                                                                   <  5:236968/148‑1 (MQ=255)
                                                                                    cAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTAtgt                                                                 <  5:236485/148‑1 (MQ=255)
                                                                                        aCCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTAt                                                             <  3:134968/148‑1 (MQ=255)
                                                                                         ccAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCg                                                                            <  7:171958/132‑1 (MQ=255)
                                                                                         ccAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCg                                                                            >  8:171958/1‑132 (MQ=255)
                                                                                         ccAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATgg                                                           <  1:202467/149‑1 (MQ=255)
                                                                                          cAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGc                                                          >  4:111816/1‑149 (MQ=255)
                                                                                            gAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCTAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAAc                                                                                           >  6:211066/1‑114 (MQ=255)
                                                                                            gAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGTGTCCAAGGTAttac                                                                                                                                       >  2:181608/1‑68 (MQ=37)
                                                                                            gAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGTGTCCAAGGTAttac                                                                                                                                       <  1:181608/70‑3 (MQ=37)
                                                                                                  aaaGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGt                                                  >  1:19577/1‑149 (MQ=255)
                                                                                                  aaaGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGt                                                  >  2:215833/1‑149 (MQ=255)
                                                                                                    aGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGGGTTGAGGTTg                                                >  6:203867/1‑149 (MQ=255)
                                                                                                    aGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGtt                                                 >  7:93449/1‑148 (MQ=255)
                                                                                                      cgcgTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGAt                                              <  2:19577/149‑1 (MQ=255)
                                                                                                            tgGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTgg                                        <  1:105777/149‑1 (MQ=255)
                                                                                                              gCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTgg                                        >  4:2948/1‑147 (MQ=255)
                                                                                                              gCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGa                                      <  1:215833/149‑1 (MQ=255)
                                                                                                                   ggTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTg                                  <  3:241769/148‑1 (MQ=255)
                                                                                                                     ttcgttcCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGc                                                                          >  2:262638/1‑106 (MQ=255)
                                                                                                                     ttcgttcCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGAt                                              >  2:238584/1‑134 (MQ=255)
                                                                                                                     ttcgttcCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGAt                                              <  1:238584/134‑1 (MQ=255)
                                                                                                                       cgttcCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGt                             >  4:214256/1‑149 (MQ=255)
                                                                                                                                  cTCCCGTAATACGTTGGACACCAAGGAATTCGTTGGTGGTGCTTTGTTTGCCACTACAGCCGGTTCGGCTGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCaagaag                  <  7:94623/149‑1 (MQ=255)
                                                                                                                                   tCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGACGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAgg                 >  4:333557/1‑149 (MQ=255)
                                                                                                                                   tCCAGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGCTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAgg                 >  8:305162/1‑149 (MQ=255)
                                                                                                                                     ccGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAgg                 <  8:215114/147‑1 (MQ=255)
                                                                                                                                                 ggACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCaa     <  3:111816/147‑1 (MQ=255)
                                                                                                                                                 ggACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCAAg    <  3:333557/148‑1 (MQ=255)
                                                                                                                                                   acacCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCAAGCt  <  7:305162/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGGCTCCGGCCAACATCTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCAAGCT  >  NC_002947/3426344‑3426638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑