Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,426,493	A→T	9.9%	T148S (ACC→TCC)	PP_3042 →	terminase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,426,493	0	A	T	9.9%	166.8 / 6.7	60	T148S (ACC→TCC)	PP_3042	terminase large subunit
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base T (3/3);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATCTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCAAGCT  >  NC_002947/3426356‑3426638                                                                                                                                          |                                                                                                                                                  aCATCTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATTCCTTGGTGTCCAAGGTAtta                                                                                                                                        >  4:271806/1‑148 (MQ=255)  cATCTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGAAATACCTTGGACACCAAGGAATTc                                                                                                                                        >  7:215114/1‑148 (MQ=255)     cTTGACGCTACTGCCCACCGGTGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAAtt                                                                                                                                         <  2:225323/144‑1 (MQ=255)     cTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAAtt                                                                                                                                         >  1:225323/1‑144 (MQ=255)        gACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATTCCTTGGTGTCCAAGGTAttacggg                                                                                                                                    <  3:271806/146‑6 (MQ=255)        gACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGTCACCAAGGAATTCGAtg                                                                                                                                    <  5:242871/146‑1 (MQ=255)        gACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGAtg                                                                                                                                    <  7:334248/146‑1 (MQ=255)           gCTACTGCCGAGCTGGGGCTTGGCGAAGCGGGTGTCTTCAGGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCGAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATggtggt                                                                                                                               <  3:218668/148‑1 (MQ=255)                   ccACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATTCCTTGGTGTCCAAGGTAttacgg                                                                                                                                     <  3:226275/134‑5 (MQ=255)                   ccACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATTCCTTGGTGTCCAAGGTAttacgg                                                                                                                                     >  4:226275/1‑130 (MQ=255)                      ccGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGc                                                                                                                    >  6:206509/1‑148 (MQ=255)                              ttGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCa                                                                                                                  >  3:105782/1‑142 (MQ=255)                              ttGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCAAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTTTTTGCCa                                                                                                                  <  4:105782/142‑1 (MQ=255)                                      gCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGtt                                                                                                                       >  2:156434/1‑129 (MQ=255)                                      gCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGtt                                                                                                                       <  1:156434/129‑1 (MQ=255)                                         ggTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAg                                                                                                <  8:93696/149‑1 (MQ=255)                                               ttCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAAcc                                                                                          >  6:236485/1‑149 (MQ=255)                                                tCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAccc                                                                                          >  8:94623/1‑148 (MQ=255)                                                tCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAAcc                                                                                          >  6:236968/1‑148 (MQ=255)                                                  aCGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTa                                                                                        >  4:146998/1‑148 (MQ=255)                                                          gCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGAtggtg                                                                                                                                 <  6:32688/99‑1 (MQ=255)                                                          gCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCt                                                                                <  3:146998/148‑1 (MQ=255)                                                          gCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCAATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCt                                                                                <  6:304667/148‑1 (MQ=255)                                                           cAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGt                                                                              >  4:241769/1‑149 (MQ=255)                                                            aaGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTc                                                                             >  2:202467/1‑149 (MQ=255)                                                               aCCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCa                                                                                                 >  1:170498/1‑126 (MQ=255)                                                               aCCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCa                                                                                                 <  2:170498/126‑1 (MQ=255)                                                                 cATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCaa                                                                                            >  6:296266/1‑129 (MQ=255)                                                                 cATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCaa                                                                                            <  5:296266/129‑1 (MQ=255)                                                                  aTTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCg                                                                       <  5:206509/149‑1 (MQ=255)                                                                      aTTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTAt                                                                   >  4:134968/1‑149 (MQ=255)                                                                       ttCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTAt                                                                   <  5:236968/148‑1 (MQ=255)                                                                         cAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTAtgt                                                                 <  5:236485/148‑1 (MQ=255)                                                                             aCCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTAt                                                             <  3:134968/148‑1 (MQ=255)                                                                              ccAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCg                                                                            <  7:171958/132‑1 (MQ=255)                                                                              ccAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCg                                                                            >  8:171958/1‑132 (MQ=255)                                                                              ccAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATgg                                                           <  1:202467/149‑1 (MQ=255)                                                                               cAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGc                                                          >  4:111816/1‑149 (MQ=255)                                                                                 gAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCTAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAAc                                                                                           >  6:211066/1‑114 (MQ=255)                                                                                 gAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGTGTCCAAGGTAttac                                                                                                                                       >  2:181608/1‑68 (MQ=37)                                                                                 gAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGTGTCCAAGGTAttac                                                                                                                                       <  1:181608/70‑3 (MQ=37)                                                                                       aaaGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGt                                                  >  1:19577/1‑149 (MQ=255)                                                                                       aaaGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGt                                                  >  2:215833/1‑149 (MQ=255)                                                                                         aGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGGGTTGAGGTTg                                                >  6:203867/1‑149 (MQ=255)                                                                                         aGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGtt                                                 >  7:93449/1‑148 (MQ=255)                                                                                           cgcgTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGAt                                              <  2:19577/149‑1 (MQ=255)                                                                                                 tgGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTgg                                        <  1:105777/149‑1 (MQ=255)                                                                                                   gCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTgg                                        >  4:2948/1‑147 (MQ=255)                                                                                                   gCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGa                                      <  1:215833/149‑1 (MQ=255)                                                                                                        ggTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTg                                  <  3:241769/148‑1 (MQ=255)                                                                                                          ttcgttcCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGc                                                                          >  2:262638/1‑106 (MQ=255)                                                                                                          ttcgttcCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGAt                                              <  1:238584/134‑1 (MQ=255)                                                                                                          ttcgttcCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGAt                                              >  2:238584/1‑134 (MQ=255)                                                                                                            cgttcCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGt                             >  4:214256/1‑149 (MQ=255)                                                                                                                       cTCCCGTAATACGTTGGACACCAAGGAATTCGTTGGTGGTGCTTTGTTTGCCACTACAGCCGGTTCGGCTGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCaagaag                  <  7:94623/149‑1 (MQ=255)                                                                                                                        tCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGACGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAgg                 >  4:333557/1‑149 (MQ=255)                                                                                                                        tCCAGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGCTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAgg                 >  8:305162/1‑149 (MQ=255)                                                                                                                          ccGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAgg                 <  8:215114/147‑1 (MQ=255)                                                                                                                                      ggACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCaa     <  3:111816/147‑1 (MQ=255)                                                                                                                                      ggACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCAAg    <  3:333557/148‑1 (MQ=255)                                                                                                                                        acacCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCAAGCt  <  7:305162/148‑1 (MQ=255)                                                                                                                                          acCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCaagaag                  >  7:137043/1‑130 (MQ=255)                                                                                                                                          acCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCaagaag                  <  8:137043/130‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                  ACATCTTGACGCTACTGCCCACCGGGGGCTTGGCGAAGCGGGTGTCTTCACGGATTGGCAAGACCATTGATTCAGTACCAGAACTGAAAGCGCGTGTGGCGTCGGTTCGTTCCAGAGACTCCCGTAATACCTTGGACACCAAGGAATTCGATGGTGGTGCATTGTTTGCCACTACAGCCGGTTCGGCAGCCAACCTATCTGAGCTGTCGGCTCGGTATGTGTATGGCGATGAGGTTGATCGCTGGGAGGTGGACGTTAACCAAGAAGGTGATCCGATCAAGCT  >  NC_002947/3426356‑3426638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑