| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,428,061 | C→G | 8.9% | S670S (TCC→TCG) ‡ | PP_3042 → | terminase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,428,061 | 0 | C | G | 8.9% | 171.5 / 7.1 | 56 | S670S (TCC→TCG) ‡ | PP_3042 | terminase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/27); new base G (3/2); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01 | |||||||||||
GCAACCGGTCGTTGAGCACCAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGAGTATCGGACGGTAGACGAACTGATCAAG > NC_002947/3427914‑3428206 | gCAACCGGTCGTTGAGCACCAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCca < 4:5426/149‑1 (MQ=255) aaCCGGTCGTTGAGCACCAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCcagc < 1:93042/149‑1 (MQ=255) aCCGGTCGTTGAGCACCAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCcag > 2:7435/1‑147 (MQ=255) gTCGTTGAGCACCAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCgg < 1:7435/149‑1 (MQ=255) gCACCAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTg > 6:301807/1‑148 (MQ=255) cACCAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTg > 6:278171/1‑147 (MQ=255) acgaaCACGATGACGACGATGACCCGGACGACGATCCAGTTTCAGCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGaa < 1:142249/145‑1 (MQ=255) ccAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAaga < 5:278171/149‑1 (MQ=255) ccAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAaga < 4:150110/149‑1 (MQ=255) aaCACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAgaga < 2:313871/149‑1 (MQ=255) acacGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCTGCTGGAGCTGCGGcgtt > 2:172643/1‑136 (MQ=255) acacGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCTGCTGGAGCTGCGGcgtt < 1:172643/139‑4 (MQ=255) gATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCt > 7:67181/1‑148 (MQ=255) gacgaTGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATgg > 3:10629/1‑149 (MQ=255) gaTGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCa > 2:171961/1‑148 (MQ=255) ccGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGaa > 8:86598/1‑125 (MQ=255) ccGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGaa < 7:86598/125‑1 (MQ=255) gacgaTCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTcc > 8:72904/1‑102 (MQ=255) gacgaTCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTcc < 7:72904/102‑1 (MQ=255) gaTCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCacac > 3:302321/1‑148 (MQ=255) tCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGACTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTc > 2:188625/1‑149 (MQ=255) gTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCgg < 5:301807/149‑1 (MQ=255) gTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCgg < 8:67181/149‑1 (MQ=255) aCCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTc < 2:200314/149‑1 (MQ=255) cGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGa < 1:171961/149‑1 (MQ=255) cAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCt > 4:43746/1‑108 (MQ=255) cAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCt < 3:43746/108‑1 (MQ=255) cAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCa < 4:43497/117‑1 (MQ=255) aaCACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCTGCTGGAGCTGCGGc < 4:33512/91‑1 (MQ=255) aaCACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCTGCTGGAGCTGCGGc > 3:33512/1‑91 (MQ=255) aaCACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGt > 6:216872/1‑149 (MQ=255) caccacCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGc > 8:234283/1‑149 (MQ=255) caccacCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGc < 4:302321/149‑1 (MQ=255) ccacgctccacTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAAc > 8:149073/1‑146 (MQ=255) ccacgctccacTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAAc < 7:149073/146‑1 (MQ=255) cacgctccacTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGgt > 2:129353/1‑149 (MQ=255) gctccacTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCata < 7:94210/92‑1 (MQ=255) gctccacTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCata > 8:94210/1‑92 (MQ=255) tccactccacCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGt < 7:320567/133‑1 (MQ=255) tccactccacCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGt > 8:320567/1‑133 (MQ=255) cactccacCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGa < 7:234283/149‑1 (MQ=255) cactccacCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGCCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGa > 5:303808/1‑149 (MQ=255) cactccacCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCAGATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGa > 5:157329/1‑149 (MQ=255) actccacCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAACGTGCGATTGCGCGTGGCGAACGGgttgtt > 6:45457/1‑145 (MQ=255) actccacCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAACGTGCGATTGCGCGTGGCGAACGGgttgtt < 5:45457/145‑1 (MQ=255) ctccacCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATa < 1:129353/149‑1 (MQ=255) cacccccgccgCCTCGCCCCGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGACACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATa < 4:10629/144‑1 (MQ=255) ccgccTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTgcgc > 3:310482/1‑119 (MQ=255) ccgccTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTgcgc < 4:310482/119‑1 (MQ=255) ccTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGAc > 3:303120/1‑145 (MQ=255) ccTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGAc < 4:303120/145‑1 (MQ=255) cGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGa < 6:303808/149‑1 (MQ=255) cTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGAGTATCg > 6:172209/1‑149 (MQ=255) tCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGAGTATCg > 5:220378/1‑148 (MQ=255) ccACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCa < 8:300178/58‑1 (MQ=255) ccACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCa > 7:300178/1‑58 (MQ=255) cATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGAGTATCGGACg > 8:212080/1‑146 (MQ=255) cATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGAGTATCGGACg < 7:212080/146‑1 (MQ=255) cAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGAGTATCGGACGGTAGAc < 5:216872/148‑1 (MQ=255) atagccgcTGCTGGAGCTGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGAGTATCGGACGGTAGACg > 6:158058/4‑148 (MQ=255) cTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGAGTATCGGACGGTAGACGAACTGATCAAg < 6:157329/149‑1 (MQ=255) | GCAACCGGTCGTTGAGCACCAACACGATGACGACGATGACCCGGACGACGATCCAGTTTCACCGCCAACACCACCGGCTCGGCCCGCTCCACGCTCCACTCCACCGCCGCCTCGCCCGGCACCTCCACCCATGCAACGCCGCAGCTCCAGCAGCGGCTATCTGAAGAGACGCTGACATGGCATATACCCAGGCACACCTCGCGGCCGTCGAGCGTGCGATTGCGCGTGGCGAACGGGTTGTTCGATACAGCGACCGCACCGTGGAGTATCGGACGGTAGACGAACTGATCAAG > NC_002947/3427914‑3428206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |