Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 338,068 A→C 7.0% S167R (AGT→AGG)  PP_0280 ← amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947338,0680AC 7.0% 216.6 / 5.9 71S167R (AGT→AGG) PP_0280amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (31/35);  new base C (2/3);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

GCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCGC  >  NC_002947/337920‑338211
                                                                                                                                                    |                                                                                                                                               
gcagcTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCa                                                                                                                                                 >  2:48495/1‑149 (MQ=255)
   gcTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGTGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTg                                                                                                                                              <  5:331023/149‑1 (MQ=255)
    cTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTgg                                                                                                                                             >  7:205379/1‑149 (MQ=255)
        aaGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGccag                                                                                                                                         <  4:89170/149‑3 (MQ=255)
         aGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCtt                                                                                                                                         <  8:205379/148‑1 (MQ=255)
          gCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCtt                                                                                                                                         >  5:34562/1‑147 (MQ=255)
          gCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       <  3:51929/149‑1 (MQ=255)
            cTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGc                                                                                                                                     <  2:115900/149‑1 (MQ=255)
             ttGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       >  8:64340/1‑146 (MQ=255)
             ttGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       <  7:64340/146‑1 (MQ=255)
             ttGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCAAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       <  7:291531/146‑1 (MQ=255)
             ttGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCAAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       >  8:291531/1‑146 (MQ=255)
                cACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCa                                                                                                                                 <  1:48495/149‑1 (MQ=255)
                   cAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGa                                                                                                                              >  8:22502/1‑149 (MQ=255)
                       aTGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCAc                                                                                                                          >  7:313448/1‑149 (MQ=255)
                           aCGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCg                                                                                                                      >  8:298391/1‑149 (MQ=255)
                              agaTCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCa                                                                                                                           <  7:316990/141‑1 (MQ=255)
                              agaTCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCa                                                                                                                           >  8:316990/1‑141 (MQ=255)
                              agaTCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTg                                                                                                                   <  6:34562/149‑1 (MQ=255)
                               gaTCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGc                                                                                                                  <  1:223056/149‑1 (MQ=255)
                                    ccAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTAc                                                                                                             >  7:124563/1‑149 (MQ=255)
                                      aGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGc                                                                                                           >  3:226488/1‑149 (MQ=255)
                                        gTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGcca                                                                                                                                          <  2:168795/116‑2 (MQ=255)
                                        gTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGcca                                                                                                                                          >  1:168795/1‑115 (MQ=255)
                                        gTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCg                                                                                                         >  3:193482/1‑149 (MQ=255)
                                         tAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCgg                                                                                                        >  3:196570/1‑149 (MQ=255)
                                          aGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCgg                                                                                                        <  4:226488/148‑1 (MQ=255)
                                              cacGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGc                                                                                                   <  7:298391/149‑1 (MQ=255)
                                              cacGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGc                                                                                                   >  4:257747/1‑149 (MQ=255)
                                              cacGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGc                                                                                                   >  5:102010/1‑149 (MQ=255)
                                              cacGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGc                                                                                                   <  4:193482/149‑1 (MQ=255)
                                                 gCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGa                                                                                                >  6:160959/1‑149 (MQ=255)
                                                 gCCTGCGGCGAAGAAGATTACAACCGGCAGGTAGGTGCGGGGGTTGATGGTCCGGGCCTTGCCGGACAGTACCTGCAGGGTCAGGGTACTGGCCAGGGCAATGCCCTTAAGCATCAGGAACCCTACGTTCGGGTACGCCGGCCGGCGGa                                                                                                >  6:346782/1‑149 (MQ=255)
                                                   cTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGa                                                                                                >  1:314542/1‑147 (MQ=255)
                                                   cTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGa                                                                                                <  2:314542/147‑1 (MQ=255)
                                                         cgAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCa                                                                                        <  2:41313/149‑1 (MQ=255)
                                                           aagaagATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAgc                                                                                      >  8:9789/1‑149 (MQ=255)
                                                             gaagaTTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAgcgc                                                                                    >  7:173544/1‑149 (MQ=255)
                                                               agaTTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGt                                                                                  <  3:54803/149‑1 (MQ=255)
                                                                  tttCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGcca                                                                                                                                          <  4:154899/90‑2 (MQ=255)
                                                                  tttCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGcca                                                                                                                                          >  3:154899/1‑89 (MQ=255)
                                                                    tCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcag                                                                             >  2:317915/1‑149 (MQ=255)
                                                                     ccACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATc                                                                                                                            >  3:112906/1‑101 (MQ=255)
                                                                     ccACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATc                                                                                                                            <  4:112906/101‑1 (MQ=255)
                                                                      cACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcagca                                                                           <  8:313448/149‑1 (MQ=255)
                                                                       aCCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCat                                                                          >  4:14750/1‑149 (MQ=255)
                                                                       aCCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCat                                                                          <  7:22502/149‑1 (MQ=255)
                                                                       aCCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCat                                                                          >  6:130397/1‑149 (MQ=255)
                                                                       aCCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCat                                                                          >  1:43820/1‑149 (MQ=255)
                                                                          ggCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCatgat                                                                       <  8:173544/149‑1 (MQ=255)
                                                                          ggCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCatgat                                                                       <  7:9789/149‑1 (MQ=255)
                                                                               gtaggtGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTa                                                                    <  1:338484/147‑1 (MQ=255)
                                                                               gtaggtGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTa                                                                    >  2:338484/1‑147 (MQ=255)
                                                                                        ggCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGttt                                                         <  3:257747/149‑1 (MQ=255)
                                                                                               atgGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGCGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCAc                                                   <  2:43820/148‑1 (MQ=255)
                                                                                                   tACGTGCCATGCCGTTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGAc                                              <  5:346782/149‑1 (MQ=255)
                                                                                                     cGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACAt                                            <  6:102010/149‑1 (MQ=255)
                                                                                                     cGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACAt                                            <  5:160959/149‑1 (MQ=255)
                                                                                                       ggCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGc                                          >  1:343749/1‑149 (MQ=255)
                                                                                                          cATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCa                                       <  3:325277/149‑1 (MQ=255)
                                                                                                              ccGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAgcg                                    <  2:343749/148‑1 (MQ=255)
                                                                                                                ggTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGccc                                 <  4:196570/149‑1 (MQ=255)
                                                                                                                    aGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGc                             <  3:14750/149‑1 (MQ=255)
                                                                                                                    aGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGc                             <  5:130397/149‑1 (MQ=255)
                                                                                                                          agcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGc                          <  7:215839/146‑1 (MQ=255)
                                                                                                                          agcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGc                          >  8:215839/1‑146 (MQ=255)
                                                                                                                            cagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCaa                     >  4:50354/1‑149 (MQ=255)
                                                                                                                                   aCGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCAGCTTCAATCTCAcc              >  3:258326/1‑149 (MQ=255)
                                                                                                                                         cTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAg         <  3:50354/148‑1 (MQ=255)
                                                                                                                                           ggCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGa      >  6:288505/1‑149 (MQ=255)
                                                                                                                                              cAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCg   >  4:92657/1‑149 (MQ=255)
                                                                                                                                               aGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCGc  <  1:317915/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCGC  >  NC_002947/337920‑338211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑