Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,490,090 G→A 8.0% A434V (GCG→GTG)  clpV ← protein ClpV1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,490,0900GA 8.0% 140.8 / 2.9 50A434V (GCG→GTG) clpVprotein ClpV1
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base A (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTCGCGCATCTTGCCGAGCAGGCCGCGCAG  >  NC_002947/3489944‑3490238
                                                                                                                                                  |                                                                                                                                                    
ccTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATgcgcgc                                                                                                                                                     >  8:184798/1‑148 (MQ=255)
ccTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACCTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATgcgcgc                                                                                                                                                     <  7:184798/148‑1 (MQ=255)
      gCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCTGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGcc                                                                                                                                              <  1:161157/149‑1 (MQ=255)
            tcAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCCCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTc                                                                                                                                         <  7:160539/147‑1 (MQ=255)
             cAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTc                                                                                                                                         >  1:208771/1‑147 (MQ=255)
             cAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGa                                                                                                                                       <  8:274577/149‑1 (MQ=255)
              acgcggcgGGCATGGTCGTGCCCCTTCCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCATGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCCCCGGGCCGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCgtggc                                                                                                                                         >  8:160539/3‑144 (MQ=255)
                gcggcgGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCa                                                                                                                                    >  2:157043/1‑149 (MQ=255)
                 cggcggGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCTATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCAc                                                                                                                                   <  7:233650/149‑1 (MQ=255)
                   gcggGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGc                                                                                                                                 <  2:208771/149‑1 (MQ=255)
                    cggGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCt                                                                                                                                <  1:157043/149‑1 (MQ=255)
                      ggCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGa                                                                                                                              >  7:49779/1‑149 (MQ=255)
                                 gCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGGGCGCCGGGCAGCGAAGTTTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCg                                                                                                                   <  5:14581/149‑1 (MQ=255)
                                   cccTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGACAATGCGCACGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCggg                                                                                                                 >  5:209866/1‑149 (MQ=255)
                                   cccTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTAGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCCGCGAAGATTGCGCCAGGACAATGCGCACGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCggg                                                                                                                 >  5:239355/1‑149 (MQ=255)
                                    ccTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGcc                                                                                                                                              <  8:228829/119‑1 (MQ=255)
                                    ccTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCggg                                                                                                                 >  3:245320/1‑148 (MQ=255)
                                        gCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCACGCGCAGGCCGTGTCGAGCACg                                                                                                                                  <  7:71672/127‑1 (MQ=255)
                                        gCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCACGCGCAGGCCGTGTCGAGCACg                                                                                                                                  >  8:71672/1‑127 (MQ=255)
                                                  tGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGt                                                                                                  <  5:201815/149‑1 (MQ=255)
                                                   ggCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGt                                                                                                  >  3:48599/1‑148 (MQ=255)
                                                    gCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTg                                                                                                                      >  5:351456/1‑127 (MQ=255)
                                                    gCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTg                                                                                                                      <  6:351456/127‑1 (MQ=255)
                                                           aCGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTTCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGc                                                                                                                                     >  7:136141/1‑105 (MQ=255)
                                                           aCGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGc                                                                                                                                     <  8:136141/105‑1 (MQ=255)
                                                           aCGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCg                                                                                         >  7:286893/1‑149 (MQ=255)
                                                             gTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTc                                                                                                                    >  5:148774/1‑120 (MQ=255)
                                                             gTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTc                                                                                                                    <  6:148774/120‑1 (MQ=255)
                                                             gTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGAt                                                                                               <  5:275096/141‑1 (MQ=255)
                                                             gTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGAt                                                                                               >  6:275096/1‑141 (MQ=255)
                                                             gTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGGCGCGCACGCTGACCGCGGTGTGGGGGAGCTGGCGGCCGGGGATGGAGCGGt                                                                                       >  4:286840/1‑149 (MQ=255)
                                                                  tttCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGGAGGCCGTTTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGGCGGTGATGTAGCGGTTGGa                                                                                   <  4:320347/148‑1 (MQ=255)
                                                                   tgccgccgGCAGATCGACAACCTGCAGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCg                                                                                                                   <  7:64851/113‑1 (MQ=38)
                                                                   tgccgccgGCAGATCGACAACCTGCAGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCg                                                                                                                   >  8:64851/3‑115 (MQ=38)
                                                                     ccGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAg                                                                                <  8:49779/148‑1 (MQ=255)
                                                                         cTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTa                                                                           >  4:134894/1‑149 (MQ=255)
                                                                             aGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGc                                                                       <  4:245320/149‑1 (MQ=255)
                                                                                      aTCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGc                                                                                          <  2:171921/121‑1 (MQ=255)
                                                                                      aTCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGc                                                                                          >  1:171921/1‑121 (MQ=255)
                                                                                      aTCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAg                                                              >  1:121621/1‑149 (MQ=255)
                                                                                               cggcAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTc                                                     <  8:286893/149‑1 (MQ=255)
                                                                                                                gcCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTtgt                                    <  4:48599/149‑1 (MQ=255)
                                                                                                                 ccGGGCAGCGAAGATTGCTCCAGGACAATGCGCACGCAGGCCGGGTCGAGCACGCTGACCGCCGTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTtgtg                                   <  6:239355/149‑1 (MQ=255)
                                                                                                                 ccGGGCAGCGAAGATTGCGCCAGGACAATGCGCACGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTtgtg                                   <  6:209866/149‑1 (MQ=255)
                                                                                                                      cAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGc                               >  4:292089/1‑148 (MQ=255)
                                                                                                                         cGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTcgc                           <  3:286840/149‑1 (MQ=255)
                                                                                                                               ttGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGAACGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTCGCGCATCt                     >  7:154761/1‑149 (MQ=255)
                                                                                                                                   gcCAGGGCAATGCGCGCACAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTCGCGCATCTTGcc                 <  4:176358/149‑1 (MQ=255)
                                                                                                                                   gcCAGGGCAATGCGCGCACAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTCGCGCATCTTGcc                 >  3:176358/1‑149 (MQ=255)
                                                                                                                                    ccAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTCGCGCATCTTGcc                 >  7:210873/1‑148 (MQ=255)
                                                                                                                                     cAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTCGCGCATCTTGcc                 >  5:207324/1‑147 (MQ=255)
                                                                                                                                            aTGCGCGCGCAGGCCGTGTCGAGCACGCTGATCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTCGCGCATCTTGCCGAGCAGGcc        <  8:154761/149‑1 (MQ=255)
                                                                                                                                                  gcgcAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTCGCGCATCTTGCCGAGCAGGCCGCgcag  >  1:165186/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCTGCAGCGATTCCAGGCGGCGGGCATGGTCGTGCCCCTTGCCGGCTTCGTGGCCGAGCACGTCGATTTCCGCCTGCAGGTTGTCGATCTGCCGGCGGCAGTCTTCCAGTGCGCCGGGCAGCGAAGATTGCGCCAGGGCAATGCGCGCGCAGGCCGTGTCGAGCACGCTGACCGCCTTGTCGGGCAGCTGGCGGCCGGTGATGTAGCGGTTGGACAGGCGTACCGCCTGCACCAGCGCTTCGTCCATCACCGCCACCTTGTGGTGCTCGCGCATCTTGCCGAGCAGGCCGCGCAG  >  NC_002947/3489944‑3490238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑