Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	345,705	G→C	5.0%	A714G (GCC→GGC)	PP_0287 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	345,705	0	G	C	5.0%	259.8 / 2.1	79	A714G (GCC→GGC)	PP_0287	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (40/35);  new base C (2/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

CATACCAGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCT  >  NC_002947/345560‑345852                                                                                                                                                  |                                                                                                                                                    cATACCAGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGc                                                                                                                                                    >  8:85250/1‑147 (MQ=255)    aCCAGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCt                                                                                                                                               >  6:139858/1‑149 (MQ=255)      cAGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGcc                                                                                                                                                   <  8:81587/143‑1 (MQ=255)      cAGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGcc                                                                                                                                                   >  7:81587/1‑143 (MQ=255)        ctCCAGCGCAGTGCTGGAGAACTGCTCGGGACTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggc                                                                                                                                           >  1:202856/2‑149 (MQ=255)        ctCCAGCGCAGTGCTGGAGAACTGCTCGGGACTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggc                                                                                                                                           >  1:208005/2‑149 (MQ=255)           cAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggcgg                                                                                                                                         >  3:233358/1‑148 (MQ=255)           cAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggcgg                                                                                                                                         >  5:90376/1‑148 (MQ=255)           cAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggcgg                                                                                                                                         >  6:286750/1‑148 (MQ=255)           cAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCGTggcgg                                                                                                                                         >  1:218570/1‑148 (MQ=255)             gCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGCCAAGCTGGCCGGCaa                                                                                                                                      <  7:24354/149‑1 (MQ=255)                cTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCt                                                                                                                                   <  5:139858/149‑1 (MQ=255)                cTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCCTGGCGGCAACCt                                                                                                                                   <  2:218570/149‑1 (MQ=255)                        gagaACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATTTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCa                                                                                                                             <  8:246435/147‑1 (MQ=255)                        gagaACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATGTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGcc                                                                                                                               >  7:246435/1‑145 (MQ=255)                        gagaACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGCCAAGCTGGCCGGCAAccgcc                                                                                                                                 >  2:114885/1‑140 (MQ=255)                        gagaACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGCCAAGCTGGCCGGCAAccgcc                                                                                                                                 <  1:114885/143‑4 (MQ=255)                          gaACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGcc                                                                                                                         >  1:260149/1‑149 (MQ=255)                           aaCTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCg                                                                                                                        <  2:45653/149‑1 (MQ=255)                            aCTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGt                                                                                                                       >  5:196613/1‑149 (MQ=255)                             cTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGtt                                                                                                                      <  5:286750/149‑1 (MQ=255)                              tGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCtt                                                                                                                                              >  8:68955/1‑124 (MQ=255)                              tGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCtt                                                                                                                                              <  7:68955/124‑1 (MQ=255)                                cTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTg                                                                                                                                 <  7:68515/135‑1 (MQ=255)                                cTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTg                                                                                                                                 >  8:68515/1‑135 (MQ=255)                                cTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTg                                                                                                                   >  4:261683/1‑149 (MQ=255)                                 tCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCCGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggc                                                                                                                                           <  7:283866/124‑1 (MQ=255)                                 tCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCCGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggc                                                                                                                                           >  8:283866/1‑124 (MQ=255)                                   ggggCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTc                                                                                                                <  4:233358/149‑1 (MQ=255)                                             ggTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCa                                                                                                       <  7:59491/148‑1 (MQ=255)                                              gTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAgg                                                                                                     >  3:146680/1‑149 (MQ=255)                                                     aGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTgc                                                                                              >  3:199364/1‑149 (MQ=255)                                                        cccTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCt                                                                                                  >  7:179615/1‑142 (MQ=255)                                                        cccTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCt                                                                                                  <  8:179615/142‑1 (MQ=255)                                                         ccTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGcc                                                                                           >  4:64143/1‑148 (MQ=255)                                                               gcgcGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAgg                                                                                                     >  6:218004/1‑132 (MQ=255)                                                               gcgcGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAgg                                                                                                     <  5:218004/132‑1 (MQ=255)                                                                  cgTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGc                                                                                 <  4:146680/149‑1 (MQ=255)                                                                      tttCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTgc                                                                                              >  8:52051/1‑132 (MQ=255)                                                                      tttCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTgc                                                                                              <  7:52051/132‑1 (MQ=255)                                                                            ttCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAg                                                                                  >  3:144834/1‑138 (MQ=255)                                                                            ttCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAg                                                                                  <  4:144834/138‑1 (MQ=255)                                                                                 ggCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAgcg                                                                   >  6:217257/1‑148 (MQ=255)                                                                                      aCTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAgggg                                                             <  1:238423/149‑1 (MQ=255)                                                                                      aCTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAgggg                                                             <  3:261683/149‑1 (MQ=255)                                                                                      aCTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAggg                                                              <  2:260149/148‑1 (MQ=255)                                                                                      aCTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAggg                                                              <  6:196613/148‑1 (MQ=255)                                                                                        tttGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAgg                                                                                                                           <  6:151597/85‑1 (MQ=255)                                                                                        tttGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGCCCACGGCAGCGCAGGGGGa                                                           >  4:117470/1‑149 (MQ=255)                                                                                          tGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGAc                                                          <  6:90376/148‑1 (MQ=255)                                                                                           gTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACt                                                         <  3:64143/148‑1 (MQ=255)                                                                                               ccAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCaa                                                     >  5:246082/1‑148 (MQ=255)                                                                                                cAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAaca                                                   >  6:131897/1‑149 (MQ=255)                                                                                                    tttttCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTg                                                                                                                   >  4:133468/1‑81 (MQ=255)                                                                                                    tttttCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTg                                                                                                                   <  3:133468/81‑1 (MQ=255)                                                                                                           tCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcg                                        >  6:124984/1‑149 (MQ=255)                                                                                                           tCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcg                                        >  3:142328/1‑149 (MQ=255)                                                                                                             aaGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgtt                                      >  4:187988/1‑149 (MQ=255)                                                                                                              aGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACAccc                                                >  3:256781/1‑138 (MQ=255)                                                                                                              aGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACAccc                                                <  4:256781/138‑1 (MQ=255)                                                                                                                tttttCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcgt                                   <  7:85250/149‑1 (MQ=255)                                                                                                                  tttCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcgtt                                  <  4:199364/148‑1 (MQ=255)                                                                                                                      aTCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGACCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATaa                                          <  3:131303/136‑1 (MQ=255)                                                                                                                      aTCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGACCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATaa                                          >  4:131303/1‑136 (MQ=255)                                                                                                                        cGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTgg                           >  1:126996/1‑149 (MQ=255)                                                                                                                           tCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACAcc                                                 <  7:266723/124‑1 (MQ=255)                                                                                                                           tCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACAcc                                                 >  8:266723/1‑124 (MQ=255)                                                                                                                             ttGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcgt                                   <  8:123589/136‑1 (MQ=255)                                                                                                                             ttGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcgt                                   >  7:123589/1‑136 (MQ=255)                                                                                                                                 cTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGc                      >  1:169067/1‑145 (MQ=255)                                                                                                                                 cTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGc                      <  2:169067/145‑1 (MQ=255)                                                                                                                                  tttCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCt                             >  5:286033/1‑137 (MQ=255)                                                                                                                                  tttCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCt                             <  6:286033/137‑1 (MQ=255)                                                                                                                                   ttCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTc                >  4:141695/1‑149 (MQ=255)                                                                                                                                      aGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAac                                                    >  4:332141/1‑110 (MQ=255)                                                                                                                                        gCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCa           <  6:246082/149‑1 (MQ=255)                                                                                                                                        aaggTTGCCGCCAAGCTGGCCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCa           >  1:134172/3‑149 (MQ=255)                                                                                                                                          ggTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATg         <  5:131897/149‑1 (MQ=255)                                                                                                                                                cGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGc   <  5:217257/149‑1 (MQ=255)                                                                                                                                                 ggCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCt  <  4:142328/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CATACCAGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCT  >  NC_002947/345560‑345852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑