Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,498,241 G→A 8.7% intergenic (‑645/+61) PP_5562 ← / ← PP_5563 hypothetical protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,498,2410GA 8.7% 128.8 / 2.5 46intergenic (‑645/+61)PP_5562/PP_5563hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base A (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCACCGTCGCAGCCGCCACCGCATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAG  >  NC_002947/3498101‑3498389
                                                                                                                                            |                                                                                                                                                    
aTTCACCGTCGCAGCCGCCACCGCATTCGCTGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCAc                                                                                                                                               <  1:36581/148‑1 (MQ=255)
               cgccACCGCATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGc                                                                                                                               <  8:30274/149‑1 (MQ=255)
                        aTTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCa                                                                                                                                 <  7:119241/138‑1 (MQ=255)
                        aTTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCa                                                                                                                                 >  8:119241/1‑138 (MQ=255)
                           cgcgGGCAAGTTCTCTTCTACATCGAGCGGTGGTGTGTCCACGTTTTATGATCGGGTTTACTTGCGAATAGGCAGGACCTTCCGTTCAATATCCTTGTTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGGCTGCCCGCGaa                                                                                                                    <  8:280812/148‑1 (MQ=12)
                                  aaGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGgcg                                                                                                            >  6:82877/1‑149 (MQ=255)
                                             tACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGgc                                                                                                             <  1:14560/137‑1 (MQ=255)
                                             tACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGgc                                                                                                             >  2:14560/1‑137 (MQ=255)
                                                aTCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCa                                                                                                <  8:19446/147‑1 (MQ=255)
                                                aTCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCa                                                                                                >  7:19446/1‑147 (MQ=255)
                                                aTCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCa                                                                                                <  6:302600/147‑1 (MQ=255)
                                                aTCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCa                                                                                                >  5:302600/1‑147 (MQ=255)
                                                aTCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATc                                                                                              <  3:68743/149‑1 (MQ=255)
                                                aTCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATc                                                                                              >  4:68743/1‑149 (MQ=255)
                                                                gCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATc                                                                              >  3:297967/1‑149 (MQ=255)
                                                                              gCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGACACTATCCCTGGTCATTCACCGTCACAACCGCCAACGCATTCGCGGGCAGCCTGCACGCCAACACCCGCGCAGCCGCTTTACTCAAACTAAACCACCATCGACCAGTACGCaaa                                                                >  3:68271/1‑149 (MQ=21)
                                                                               cTGGTTTACCCGCGAATACGCCGGACGTGCCTGCCACTATCCCTTGTCATTCACCGTCACAACCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAAc                                                               <  4:68271/149‑1 (MQ=38)
                                                                                 ggTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGt                                                             >  7:257361/1‑149 (MQ=255)
                                                                                       cccGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTg                                                        <  8:257361/148‑1 (MQ=255)
                                                                                        ccGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGc                                                       >  5:43050/1‑148 (MQ=255)
                                                                                             aaTAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGa                                                 <  5:82877/149‑1 (MQ=255)
                                                                                                    cGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCtt                                          >  5:126525/1‑149 (MQ=255)
                                                                                                             cGGCCACTATCCCTGGTCATTCACCGTCACAACCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGcc                                  >  3:41602/1‑148 (MQ=255)
                                                                                                                  aCTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTctct                                                         >  1:81868/1‑120 (MQ=255)
                                                                                                                  aCTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTctct                                                         <  2:81868/120‑1 (MQ=255)
                                                                                                                  aCTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCAt                                                    <  8:317699/125‑1 (MQ=255)
                                                                                                                  aCTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCAt                                                    >  7:317699/1‑125 (MQ=255)
                                                                                                                   cTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTc                           <  1:138294/149‑1 (MQ=255)
                                                                                                                        ccTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCa                                                     <  4:163667/118‑1 (MQ=255)
                                                                                                                         cTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGAt                     >  3:335662/1‑149 (MQ=255)
                                                                                                                               aTTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGAt                     <  6:214488/143‑1 (MQ=255)
                                                                                                                               aTTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGAt                     >  5:214488/1‑143 (MQ=255)
                                                                                                                               aTTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATg               <  4:297967/149‑1 (MQ=255)
                                                                                                                                 tCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGa              >  2:169807/1‑148 (MQ=255)
                                                                                                                                   aCCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTc                           <  1:323079/133‑1 (MQ=255)
                                                                                                                                   aCCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTc                           >  2:323079/1‑133 (MQ=255)
                                                                                                                                    ccGTCACAACCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCg          <  4:41602/149‑1 (MQ=255)
                                                                                                                                         acaGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGtct                                                           >  2:296948/1‑95 (MQ=255)
                                                                                                                                         acaGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGtct                                                           >  2:292852/1‑95 (MQ=255)
                                                                                                                                         acaGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCt                                           >  6:308838/1‑111 (MQ=255)
                                                                                                                                         acaGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCt                                           <  5:308838/111‑1 (MQ=255)
                                                                                                                                         acaGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCtt      <  4:335662/148‑1 (MQ=255)
                                                                                                                                         acaGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCtt      <  6:43050/148‑1 (MQ=255)
                                                                                                                                         acaGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCAACGATTGCCTTGCATCGCCAATTGTCGATGATGTAATGATCCGGCtt      >  6:191667/1‑148 (MQ=255)
                                                                                                                                           aGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGaa   <  6:126525/149‑1 (MQ=255)
                                                                                                                                           aGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGACGGTTTCATCCAACACCACGACCATCGCCCAGTACGCAAACGTCTCTGCCAACGATAGCCTTGCATGGCCAATTCTCGGTGATGTAATGATCCGGCTAGaa   >  5:151025/1‑149 (MQ=255)
                                                                                                                                            gccgccACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAg  >  1:46663/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
ATTCACCGTCGCAGCCGCCACCGCATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAG  >  NC_002947/3498101‑3498389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑