Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,507,747 C→A 8.3% F32L (TTC→TTA) ‡ PP_3105 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,507,7470CA 8.3% 147.3 / 4.1 48F32L (TTC→TTA) ‡PP_3105membrane protein
Reads supporting (aligned to +/- strand):  ref base C (23/21);  new base A (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

GCTGGGCACTACCCTGGGTAATTTCTTGACGGGGAAGGATTAAATGTTTACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGTTGCACCAATGTTC  >  NC_002947/3507609‑3507879
                                                                                                                                          |                                                                                                                                    
gCTGGGCACTACCCTGGGTAATTTCTTGACGGGGAAGGATTAAATGTTTACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGc                                                                                                                            <  6:59539/149‑1 (MQ=255)
    ggCACTACCCTGGGTAATTTCTTGACGGGGAAGGATTAAATGTTTACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCg                                                                                                                          <  2:142139/147‑1 (MQ=255)
              tGGGTAATTTCTTGACGGGGAAGGATTAAATGTTTACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGAt                                                                                                               >  1:185574/1‑148 (MQ=255)
                        cTTGACGGGGAAGGATTAAATGTTTACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGtt                                                                                                     <  2:185574/148‑1 (MQ=255)
                                           aTGTTTACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCACCGCCATGAATGGCGgcgc                                                                                                                            <  1:118709/106‑1 (MQ=255)
                                           aTGTTTACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCACCGCCATGAATGGCGgcgc                                                                                                                            >  2:118709/1‑106 (MQ=255)
                                              tttACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCACTGATGATGAACGGATTGGCTCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATtg                                                                              >  5:355155/1‑149 (MQ=255)
                                               ttACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGCTCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATtgt                                                                             >  1:346012/1‑149 (MQ=255)
                                                tACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATtgtg                                                                            >  3:247868/1‑149 (MQ=255)
                                                      aGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGAt                                                                                                               >  1:222275/1‑108 (MQ=255)
                                                      aGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGAt                                                                                                               <  2:222275/108‑1 (MQ=255)
                                                           gTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGa                                                                                                 >  4:91297/1‑117 (MQ=255)
                                                           gTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGa                                                                                                 <  3:91297/117‑1 (MQ=255)
                                                           gTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGt                                                                 <  3:48370/149‑1 (MQ=255)
                                                            tttttttAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGt                                                                 >  3:40478/1‑148 (MQ=255)
                                                              tttttAAAAATATGTGGTCGGTGTGGGTGCTGGTCGTGATATTGGACCTTTTGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCTTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGa                                                               <  6:355155/148‑1 (MQ=255)
                                                                 ttAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCtg                                                                                            >  8:139539/1‑116 (MQ=255)
                                                                 ttAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCAc                                                           >  3:335728/1‑149 (MQ=255)
                                                                 ttAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCAc                                                           >  3:49740/1‑149 (MQ=255)
                                                                    aaaaaaTCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATc                                                                                   >  2:120288/1‑122 (MQ=255)
                                                                        aaTCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGt                                                      >  6:182791/1‑147 (MQ=255)
                                                                        aaTCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTaa                                                    <  5:182791/149‑1 (MQ=255)
                                                                        aaTCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTaa                                                    <  5:144292/149‑1 (MQ=255)
                                                                          tCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAAt                                                                                <  2:196335/119‑1 (MQ=255)
                                                                          tCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGGTTCCCGCAGTGAGCACTTTGTAAGt                                                  >  6:95354/1‑149 (MQ=255)
                                                                            tGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCAt                                                                                    >  7:77393/1‑113 (MQ=255)
                                                                            tGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCAt                                                                                    <  8:77393/113‑1 (MQ=255)
                                                                            tGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGtt                                                 >  8:33354/1‑148 (MQ=255)
                                                                              gTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGttt                                                <  7:33354/147‑1 (MQ=255)
                                                                              gTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTcg                                              >  8:193282/1‑149 (MQ=255)
                                                                              gTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATAAATTTTGGTTACCGCAGTGAGAACTTTGTAAGTTTcg                                              >  7:327649/1‑149 (MQ=255)
                                                                               tCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTcgc                                             <  4:247868/149‑1 (MQ=255)
                                                                                   tgtgGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAAt                                         <  1:346159/149‑1 (MQ=255)
                                                                                         tgctgGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAAtt                                                                               >  8:292352/1‑105 (MQ=255)
                                                                                         tgctgGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAAtt                                                                               <  7:292352/105‑1 (MQ=255)
                                                                                           ctgGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTgcgc                                   <  4:49740/147‑1 (MQ=255)
                                                                                           ctgGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTgcgc                                   <  4:335728/147‑1 (MQ=255)
                                                                                                 gTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGttct                            <  7:193282/148‑1 (MQ=255)
                                                                                                             cTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACttt                                                        <  1:151001/108‑1 (MQ=255)
                                                                                                             cTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACttt                                                        >  2:151001/1‑108 (MQ=255)
                                                                                                                atgatgaCCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCTATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGtt              <  4:40478/147‑1 (MQ=255)
                                                                                                                   atgaCCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGTTGCAc          >  1:332982/1‑148 (MQ=255)
                                                                                                                     gaCCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGAGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGTTGCACCaa       >  2:232117/1‑149 (MQ=255)
                                                                                                                       ccGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCAGTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGTTGCACCAATg     >  6:127452/1‑149 (MQ=255)
                                                                                                                                 gccgccATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGTTGCAc          <  1:212277/134‑1 (MQ=255)
                                                                                                                                 gccgccATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGTTGCAc          >  2:212277/1‑134 (MQ=255)
                                                                                                                                  ccgccATGAATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGTTGCACCAATGTTc  >  2:172962/1‑141 (MQ=255)
                                                                                                                                  ccgccATGAATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGTTGCACCAATGTTc  <  1:172962/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                    
GCTGGGCACTACCCTGGGTAATTTCTTGACGGGGAAGGATTAAATGTTTACCACAGAACGTTTTTTTAAAAAAATCTGGTCGGTGTGGCTGCTGGTCGTGATATTGGCCCTGATGATGACCGGAGTGGCGCCGCCATTCATGGCGGTGCCGGCGATCCTGATCATTATCGTTATGACGCTGTGGTGCATCAATTGTGCTTACCGCAGTGAGCACTTTGTAAGTTTCGCGAATTTGCGCATGTTCTTCAATATGTCGGTTGCACCAATGTTC  >  NC_002947/3507609‑3507879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑