Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,532,631 T→G 9.7% A184A (GCA→GCC)  PP_3121 ← transcriptional regulator, LysR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,532,6310TG 9.7% 173.0 / 10.8 62A184A (GCA→GCC) PP_3121transcriptional regulator, LysR family
Reads supporting (aligned to +/- strand):  ref base T (26/30);  new base G (3/3);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

GCCACCATGCGGCCGATGGTCGTCAGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCG  >  NC_002947/3532488‑3532774
                                                                                                                                               |                                                                                                                                               
gCCACCATGCGGCCGATGGTCGTCAGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGcc                                                                                                                                              <  2:86766/147‑1 (MQ=255)
      aTGCGGCCGATGGTCGTCAGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAAGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGAc                                                                                                                                      <  1:193465/149‑1 (MQ=255)
         cGGCCGATGGTCGTCAGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTg                                                                                                                                   <  7:157865/149‑1 (MQ=255)
         cGGCCGATGGTCGTCAGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGGGCACAGCTGatgg                                                                                                                                    <  1:40681/148‑4 (MQ=255)
                     gtcAGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGGGCACAGCTGatg                                                                                                                                     <  4:325801/135‑3 (MQ=255)
                     gtcAGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGGGCACAGCTGatg                                                                                                                                     >  3:325801/1‑133 (MQ=255)
                        aGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGcc                                                                                                                      >  1:257747/1‑147 (MQ=255)
                        aGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGcc                                                                                                                      >  3:248414/1‑147 (MQ=255)
                        aGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGcc                                                                                                                      >  3:231983/1‑147 (MQ=255)
                           tggtggCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAggg                                                                                                                 >  3:70475/1‑149 (MQ=255)
                               gtcTTTCGAACGAGACTGCCATCTTGACGTGGCGGGCCGCCACATTCTGATCACTCAGCTGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTg                                                                                                                                   <  1:160834/125‑1 (MQ=255)
                               ggCTTTCGAACGAGACTGCCAGATTGCCGTGGCGGGCCGCCACCTTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGAAACGAAATCTTCGGCCATCAACTGTGCCAATCTGAccag                                                                                                                                   >  2:160834/1‑125 (MQ=255)
                                     cGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTcgac                                                                                                        <  8:6867/148‑3 (MQ=255)
                                      gAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTcggcgg                                                                                                      >  3:198937/1‑149 (MQ=255)
                                      gAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTcggcg                                                                                                       <  1:17505/148‑1 (MQ=255)
                                      gAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTcggcg                                                                                                       <  1:181459/148‑1 (MQ=255)
                                              cTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACg                                                                                              <  4:248414/149‑1 (MQ=255)
                                              cTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACg                                                                                              <  4:231983/149‑1 (MQ=255)
                                              cTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCCCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACg                                                                                              <  2:163932/149‑1 (MQ=255)
                                               tGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACgg                                                                                             >  2:2337/1‑149 (MQ=255)
                                                        gCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGGGCACAGCTGatgg                                                                                                                                    <  6:364068/101‑4 (MQ=255)
                                                        gCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGGGCACAGCTGatgg                                                                                                                                    >  5:364068/1‑98 (MQ=255)
                                                         ccGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGc                                                                                    <  2:257747/148‑1 (MQ=255)
                                                         ccGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGc                                                                                    <  1:2337/148‑1 (MQ=255)
                                                         ccGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCg                                                                                   >  2:331495/1‑149 (MQ=255)
                                                          cGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGAc                                                                                                                                      <  8:123330/97‑1 (MQ=255)
                                                          cGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGAc                                                                                                                                      >  7:123330/1‑97 (MQ=255)
                                                                gggCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCt                                                                                                                                    <  2:315562/93‑1 (MQ=255)
                                                                gggCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCt                                                                                                                                    >  1:315562/1‑93 (MQ=255)
                                                                   ccgccACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCAGTCCATGTa                                                                          >  4:115685/1‑148 (MQ=255)
                                                                    cgccACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATg                                                                            >  6:296434/1‑145 (MQ=255)
                                                                    cgccACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATg                                                                            <  5:296434/145‑1 (MQ=255)
                                                                          aTTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGt                                                                  <  4:198937/149‑1 (MQ=255)
                                                                          aTTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCGGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGt                                                                  <  4:283967/149‑1 (MQ=255)
                                                                               gCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAg                                                                                     <  3:228549/125‑1 (MQ=255)
                                                                               gCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAg                                                                                     >  4:228549/1‑125 (MQ=255)
                                                                                cTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCAGTCCATGTACAACGGGTGGaa                                                              <  3:115685/147‑1 (MQ=255)
                                                                                   cATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCTCGAAATCTTCGGCCATCAGCTGTGCCCAGGTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCa                                                         <  5:143743/149‑1 (MQ=255)
                                                                                   cATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAg                                                                                                                   <  5:356047/91‑1 (MQ=255)
                                                                                   cATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAg                                                                                                                   >  6:356047/1‑91 (MQ=255)
                                                                                   cATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTa                                                                          <  1:230504/132‑1 (MQ=255)
                                                                                   cATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTa                                                                          >  2:230504/1‑132 (MQ=255)
                                                                                   cATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGGCCa                                                         >  2:124336/1‑149 (MQ=255)
                                                                                        gcagGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAg                                                                                     >  1:307332/1‑116 (MQ=255)
                                                                                        gcagGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAg                                                                                     <  2:307332/116‑1 (MQ=255)
                                                                                        gcagGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCa                                                    >  4:66430/1‑149 (MQ=255)
                                                                                        gcagGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCa                                                    >  5:335489/1‑149 (MQ=255)
                                                                                            gcgcACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACg                                                                                        <  2:31265/109‑1 (MQ=255)
                                                                                            gcgcACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACg                                                                                        >  1:31265/1‑109 (MQ=255)
                                                                                            gcgcACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGa                                                <  3:66430/149‑1 (MQ=255)
                                                                                                 ccgccgAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTa                                           >  6:332208/1‑149 (MQ=255)
                                                                                                     cgAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCg                                       >  2:40283/1‑149 (MQ=255)
                                                                                                              cTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGc                              <  6:335489/149‑1 (MQ=255)
                                                                                                                     gCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTcc                                                                               <  4:39829/93‑1 (MQ=255)
                                                                                                                     gCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTcc                                                                               >  3:39829/1‑93 (MQ=255)
                                                                                                                       cACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGcc                       >  2:292175/1‑147 (MQ=255)
                                                                                                                       cACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGcc                       <  1:292175/147‑1 (MQ=255)
                                                                                                                                  cGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGAc              <  1:40283/145‑1 (MQ=255)
                                                                                                                                        tCAGCTGGGCACAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATgg    >  1:256137/1‑149 (MQ=255)
                                                                                                                                          aGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATg     >  1:201505/1‑146 (MQ=255)
                                                                                                                                          aGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATg     <  2:201505/146‑1 (MQ=255)
                                                                                                                                           gCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCg  >  4:280099/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GCCACCATGCGGCCGATGGTCGTCAGCTGGTGGCTTTCGAACGCGACTGCCAGCTTGCCGTGGCGGGCCGCCACATTCTGCTCCATCAGCAGGCGCACCGCCGAAGGGCGCTGCAGGGCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCG  >  NC_002947/3532488‑3532774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑