Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,534,768	C→A	9.1%	intergenic (+55/‑100)	atoB → / → PP_3124	3‑oxoacid CoA‑transferase subunit B/short chain fatty acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,534,768	0	C	A	9.1%	159.4 / 6.8	55	intergenic (+55/‑100)	atoB/PP_3124	3‑oxoacid CoA‑transferase subunit B/short chain fatty acid transporter
Reads supporting (aligned to +/- strand):  ref base C (24/25);  new base A (2/3);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01

AGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTTGCCTTGC  >  NC_002947/3534622‑3534913                                                                                                                                                   |                                                                                                                                                  agaCCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACAt                                                                                                                                                 <  7:170163/149‑1 (MQ=255) agaCCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACAt                                                                                                                                                 <  7:175124/149‑1 (MQ=255)     cgcgCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGAc                                                                                                                                             >  7:211718/1‑149 (MQ=255)      gcgcCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGAcc                                                                                                                                            <  2:132460/149‑1 (MQ=255)                  aGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGGCGGTCAATGTACCGACGCATTcgc                                                                                                                                 <  1:182223/148‑1 (MQ=38)                  aGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGCTTACCCGCGAATGCGGCGGTCAATGTACCGACGCATTcgc                                                                                                                                 <  5:129200/148‑1 (MQ=38)                   gCGTCGAGGAGATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCgg                                                                                                                               <  2:227625/149‑1 (MQ=255)                   gCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCgg                                                                                                                               <  5:29784/149‑1 (MQ=255)                               tcatcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTc                                                                                                                   >  4:59340/1‑149 (MQ=255)                                  tcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAGTGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGc                                                                                                                         >  7:278890/1‑140 (MQ=255)                                  tcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAGTGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGc                                                                                                                         <  8:278890/140‑1 (MQ=255)                                      gaAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAgg                                                                                                            >  6:201050/1‑149 (MQ=255)                                                   aaaCTGATCGTGCCGTATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTATTc                                                                                                                   <  2:219221/129‑1 (MQ=255)                                                        gATCGTGCCGGATGATGTGAATGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGCCCGCCGCAGTCGCGGGTAAGCCTGGTCGTAAAGGTTTTTTGCGTTggatgg                                                                                           >  4:189764/1‑143 (MQ=255)                                                         aTCGTGCCGGATGATGTTAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGc                                                                                          <  2:204018/148‑1 (MQ=255)                                                             tGCCGGTTTATGTGAAGGAAATGTCCTTCTAAGGCATGTCGTGATCTCACCGTGTAGGCGCGGGTTTAGGGGCGAATGCGTCTGTACTTTGAGGGCCGCATTCGCGTGTAAGCGTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATAc                                                                                     <  2:172047/149‑1 (MQ=255)                                                                cGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTgg                                                                                           >  8:154915/1‑140 (MQ=255)                                                                cGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTgg                                                                                           <  7:154915/140‑1 (MQ=255)                                                                cGGATGATGTGAAGGAAATGACCTTCTAAGGAATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCATGGCAATACCg                                                                                   >  7:8415/1‑148 (MQ=255)                                                                   atgatgTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTc                                                                               <  5:201050/149‑1 (MQ=255)                                                                    ttatttgAAGGAAATGACCTTCTAAGGCATGACTTGATCTCACACTGTTGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCGGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTc                                                                               <  8:211718/143‑1 (MQ=39)                                                                       tgtgAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATcc                                                                           >  7:326852/1‑149 (MQ=255)                                                                       tgtgAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATc                                                                            <  3:189764/148‑1 (MQ=255)                                                                          gAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCa                                                                         >  7:239410/1‑148 (MQ=255)                                                                                  tGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCaa                                                                <  8:326852/149‑1 (MQ=255)                                                                                       ttCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATaa                                                             >  7:234554/1‑147 (MQ=255)                                                                                        tCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAg                                                          >  4:238921/1‑149 (MQ=255)                                                                                         cTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATaaaa                                                           <  1:6000/147‑1 (MQ=255)                                                                                            aGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAAt                                                      >  3:298009/1‑149 (MQ=255)                                                                                                       gATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGaa                                                       >  4:135907/1‑137 (MQ=255)                                                                                                       gATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGaa                                                       <  3:135907/137‑1 (MQ=255)                                                                                                           tcACCCTGTAGGCGCGGGTTTTCCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCt                                       >  4:153014/1‑149 (MQ=255)                                                                                                           tcACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCt                                       >  2:332209/1‑149 (MQ=255)                                                                                                            cACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATaaaa                                                           <  5:105554/128‑1 (MQ=255)                                                                                                            cACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATaaaa                                                           >  6:105554/1‑128 (MQ=255)                                                                                                              cccTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGtt                                                                                                 <  4:327878/88‑1 (MQ=255)                                                                                                              cccTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGtt                                                                                                 >  3:327878/1‑88 (MQ=255)                                                                                                              cccTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGaa                                    <  4:298009/149‑1 (MQ=255)                                                                                                               ccTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGaaa                                   >  1:57721/1‑149 (MQ=255)                                                                                                                   tAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCa                               <  1:332209/149‑1 (MQ=255)                                                                                                                               tACACGCGAATGCGTGGGTAGATTGTCCGCAGCTTTCGGGGGTAAGGCTGTTCCTAGAGGTTTTTTGCGTTGCCTGGCAATTCCGGTTCATCACATAATACCATTAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCgat                    <  8:8415/148‑3 (MQ=255)                                                                                                                                  ccGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgc                >  5:205142/1‑149 (MQ=255)                                                                                                                                   cgcgAATGCGGCGGTCAATGTACCGACGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgcg               >  7:276017/1‑149 (MQ=38)                                                                                                                                    gcgAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATc                                                                            <  7:142798/87‑1 (MQ=255)                                                                                                                                    gcgAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgcgc              >  6:230680/1‑149 (MQ=255)                                                                                                                                    gcgAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTACCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATc                                                                            >  8:142798/1‑87 (MQ=255)                                                                                                                                     cgAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgcgcg             >  3:269087/1‑149 (MQ=255)                                                                                                                                      gAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCgcgcgc            <  5:230680/149‑1 (MQ=255)                                                                                                                                      gAATGCGGCGGTCAATGTACCGACGCATTCGCGGGTAAACCCGCGCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGa                            >  7:138475/1‑133 (MQ=21)                                                                                                                                      gAATGCGGCGGTCAATGTACCGACGCATTCGCGGGTAAACCCGCGCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGa                            <  8:138475/133‑1 (MQ=21)                                                                                                                                        aTGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgc                <  7:235519/143‑1 (MQ=255)                                                                                                                                        aTGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgc                >  8:235519/1‑143 (MQ=255)                                                                                                                                            gTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTTGcc      <  8:234554/149‑1 (MQ=255)                                                                                                                                             tCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTTGCCt     <  3:59340/149‑1 (MQ=255)                                                                                                                                                gTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTTGCCTTgc  >  3:4193/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  AGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTTGCCTTGC  >  NC_002947/3534622‑3534913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑