Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,667,282 T→C 6.9% intergenic (+225/+37) PP_3230 → / ← PP_3231 phosphoribosyl transferase domain‑containing protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,667,2820TC 6.9% 165.9 / 2.6 58intergenic (+225/+37)PP_3230/PP_3231phosphoribosyl transferase domain‑containing protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/25);  new base C (2/2);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

TCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAACCAGAGCAGCGCATCGAACTG  >  NC_002947/3667135‑3667429
                                                                                                                                                   |                                                                                                                                                   
tcaatACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTg                                                                                                                                                    >  4:100019/1‑149 (MQ=255)
          gCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCatat                                                                                                                                          >  4:86479/1‑149 (MQ=255)
              aCGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGt                                                                                                                                      <  4:331005/149‑1 (MQ=255)
                         gCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCt                                                                                                                           >  7:177135/1‑149 (MQ=255)
                                gCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATgg                                                                                                                               <  1:296173/138‑1 (MQ=255)
                                gCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATgg                                                                                                                               >  2:296173/1‑138 (MQ=255)
                                     aGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCt                                                                                                                >  7:267510/1‑148 (MQ=255)
                                       gTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAg                                                                                                                                                 >  6:233430/1‑113 (MQ=255)
                                       gTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAg                                                                                                                                                 <  5:233430/113‑1 (MQ=255)
                                          actactCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATaca                                                                                                          <  3:86479/149‑1 (MQ=255)
                                            tactCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATacac                                                                                                         >  8:12524/1‑148 (MQ=255)
                                              ctctTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACAcc                                                                                                        <  8:177135/147‑1 (MQ=255)
                                               tctTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATa                                                                                                            >  8:313910/1‑142 (MQ=255)
                                               tctTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATa                                                                                                            <  7:313910/142‑1 (MQ=255)
                                                 ttCGACATGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGACTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTAGGCCGAGGCTCAGACACCAAAtt                                                                                                   >  8:300684/1‑148 (MQ=255)
                                                          ctgAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCCGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGttt                                                                                          >  5:251939/1‑149 (MQ=255)
                                                          ctgAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCCGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGttt                                                                                          >  5:247555/1‑149 (MQ=255)
                                                                tCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATa                                                                                               <  8:190368/138‑1 (MQ=255)
                                                                    tCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTgg                                                                                <  5:137215/149‑1 (MQ=255)
                                                                          ggTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGTCGCTCGCCGGTGACTGTTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAAt                                                                                                    <  7:228390/123‑1 (MQ=255)
                                                                          ggTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTc                                                                             >  7:300265/1‑146 (MQ=255)
                                                                          gctcttccGATCTAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCaa                                                                           <  5:243086/142‑1 (MQ=255)
                                                                                ccGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCtg                                                                    >  7:95970/1‑149 (MQ=255)
                                                                                  gCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCtgtt                                                                  <  3:100019/149‑1 (MQ=255)
                                                                                   cTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCtgttg                                                                 >  5:190989/1‑149 (MQ=255)
                                                                                    tCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTgg                                                                                >  1:255889/1‑133 (MQ=255)
                                                                                    tCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTgg                                                                                <  2:255889/133‑1 (MQ=255)
                                                                                      gAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGttt                                                              >  3:310948/1‑149 (MQ=255)
                                                                                      gAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGttt                                                              <  4:310948/149‑1 (MQ=255)
                                                                                        agagCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCaa                                                                           >  6:243086/1‑134 (MQ=255)
                                                                                        agagCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCCGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCg                                                            <  6:251939/149‑1 (MQ=255)
                                                                                        agagCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCCGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCg                                                            <  6:247555/149‑1 (MQ=255)
                                                                                            ccTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCaa                                                        <  8:300265/149‑1 (MQ=255)
                                                                                            ccTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCaa                                                        <  8:267510/149‑1 (MQ=255)
                                                                                             cTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAag                                                       <  6:190989/149‑1 (MQ=255)
                                                                                                gCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAaggg                                                     >  3:8434/1‑148 (MQ=255)
                                                                                                     ttGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAagggcagag                                                >  1:117479/1‑146 (MQ=255)
                                                                                                         cGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTc                                                             <  4:47354/131‑1 (MQ=255)
                                                                                                         cGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTc                                                             >  3:47354/1‑131 (MQ=255)
                                                                                                                cgcgGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCAccggcc                                    >  6:273695/1‑149 (MQ=255)
                                                                                                                cgcgGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGCCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTc                                                             >  2:287470/1‑124 (MQ=255)
                                                                                                                cgcgGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGCCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTc                                                             <  1:287470/124‑1 (MQ=255)
                                                                                                                        tCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCtgt                                                                   >  4:104389/1‑110 (MQ=255)
                                                                                                                        tCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCtgt                                                                   <  3:104389/110‑1 (MQ=255)
                                                                                                                              gTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAAc                      <  4:8434/149‑1 (MQ=255)
                                                                                                                               tGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGATTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAAcc                     >  8:229415/1‑149 (MQ=255)
                                                                                                                                   tGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCg                                                            <  5:349856/106‑1 (MQ=255)
                                                                                                                                   tGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCg                                                            >  6:349856/1‑106 (MQ=255)
                                                                                                                                     gTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAgggcagggca                                              <  7:215232/118‑1 (MQ=255)
                                                                                                                                     gTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAgggcagggca                                              >  8:215232/1‑118 (MQ=255)
                                                                                                                                     gTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAACCagag                 >  7:193344/1‑147 (MQ=255)
                                                                                                                                          tGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAACCAGAGCAGCGCa          >  1:140520/1‑149 (MQ=255)
                                                                                                                                           gAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAACCAGAGCAGCGCAt         >  6:321645/1‑149 (MQ=255)
                                                                                                                                           gAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAACCAGAGCAGCGCAt         >  4:285074/1‑149 (MQ=255)
                                                                                                                                            aaGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAACCAGAGCAGCGCAt         <  8:193344/148‑1 (MQ=255)
                                                                                                                                               tACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAACCAGAGCAGCGCATCGaa     <  7:12524/149‑1 (MQ=255)
                                                                                                                                                aCCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTc                                           <  6:4368/110‑1 (MQ=255)
                                                                                                                                                  cTGCAGCGCATATCCGTCAGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAACATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAACCAGAGCAGCGCAACGAAATg  >  5:298203/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGGGCAGTCACCGGCCGGGTCTGCTCGAACCAGAGCAGCGCATCGAACTG  >  NC_002947/3667135‑3667429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑