Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,701,978	C→T	6.1%	D136D (GAC→GAT)	PP_3267 →	Clp protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,701,978	0	C	T	6.1%	244.5 / 4.1	81	D136D (GAC→GAT)	PP_3267	Clp protease
Reads supporting (aligned to +/- strand):  ref base C (39/37);  new base T (3/2);  total (42/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

TGGGCAATATCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGA  >  NC_002947/3701836‑3702121                                                                                                                                               |                                                                                                                                                 tGGGCAATATCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTgcgc                                                                                                                                            <  7:19342/149‑1 (MQ=255)          tCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTgcgc                                                                                                                                            <  4:214360/140‑1 (MQ=255)          tCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTgcgc                                                                                                                                            >  3:214360/1‑140 (MQ=255)            ctctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGTATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCa                                                                                                                                 <  8:244399/149‑1 (MQ=255)            ctctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                   >  6:134361/1‑147 (MQ=255)            ctctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                   >  6:318040/1‑147 (MQ=255)            ctctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCa                                                                                                                                 <  5:237757/149‑1 (MQ=255)             tctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                   >  3:244118/1‑146 (MQ=255)               ttCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                   >  3:254079/1‑144 (MQ=255)                 ccTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgcgc                                                                                                                                  >  4:318293/1‑143 (MQ=255)                 ccTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATgcgc                                                                                                                                  <  3:318293/143‑1 (MQ=255)                   ttGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTg                                                                                                                          >  7:12122/1‑149 (MQ=255)                     gCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTgg                                                                                                                         <  4:254079/148‑1 (MQ=255)                     gCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGc                                                                                                                        >  1:317884/1‑149 (MQ=255)                      ccGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCg                                                                                                                       <  8:12122/149‑1 (MQ=255)                      ccGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCg                                                                                                                       <  5:134361/149‑1 (MQ=255)                             gCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCa                                                                                                                <  3:122626/149‑1 (MQ=255)                                         ttGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCgg                                                                                                    >  8:233047/1‑149 (MQ=255)                                               tttCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTgg                                                                                                                         >  1:26232/1‑122 (MQ=255)                                               tttCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTgg                                                                                                                         <  2:26232/122‑1 (MQ=255)                                                   aGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTAt                                                                                                                                      >  4:279476/1‑105 (MQ=255)                                                   aGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGtttt                                                                                          >  2:263364/1‑149 (MQ=255)                                                     cAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                         >  3:1804/1‑148 (MQ=255)                                                      aaGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                       <  1:263364/149‑1 (MQ=255)                                                      aaGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                       <  5:318040/149‑1 (MQ=255)                                                             ttttCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGt                                                                                                                                        <  2:98626/93‑1 (MQ=255)                                                             ttttCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGt                                                                                                                                        >  1:98626/1‑93 (MQ=255)                                                               ttCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACcgc                                                                               <  4:244118/148‑1 (MQ=255)                                                                  cACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGtt                                                                            <  2:317884/148‑1 (MQ=255)                                                                  cACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTa                                                                           >  8:92007/1‑149 (MQ=255)                                                                     ccGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                         >  5:13881/1‑132 (MQ=255)                                                                     ccGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                         <  6:13881/132‑1 (MQ=255)                                                                     ccGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTAt                                                                          <  3:274891/147‑1 (MQ=255)                                                                     ccGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTAt                                                                          >  4:274891/1‑147 (MQ=255)                                                                             ttGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGcc                                                                 >  7:95080/1‑148 (MQ=255)                                                                                    ttGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATcc                                                            >  3:246297/1‑146 (MQ=255)                                                                                      gCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑aa                                                         <  4:246297/146‑1 (MQ=255)                                                                                      gCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAgg                                                       <  8:95080/148‑1 (MQ=255)                                                                                          ggTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGAGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACt                                                  >  3:54971/1‑149 (MQ=255)                                                                                           gTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTg                                                 >  8:80732/1‑149 (MQ=255)                                                                                            ttCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                         <  6:77094/109‑1 (MQ=255)                                                                                              cGGTGGTCCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGgc                                               <  4:54971/148‑1 (MQ=255)                                                                                                gTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGcgcc                                            >  3:177407/1‑149 (MQ=255)                                                                                                gTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGcgcc                                            >  4:88931/1‑149 (MQ=255)                                                                                                   gACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCcgcg                                         <  3:88931/149‑1 (MQ=255)                                                                                                       aTTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAg                                     >  8:57256/1‑149 (MQ=255)                                                                                                           gcgGATGTCCGAGTATGCGATGAGACTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGgcgc                                 >  8:75118/1‑149 (MQ=255)                                                                                                            cgGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCt                                <  4:177407/149‑1 (MQ=255)                                                                                                            cgGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCt                                <  7:233047/149‑1 (MQ=255)                                                                                                              gATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATCTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTgg                              <  4:1804/149‑1 (MQ=255)                                                                                                                   ccGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAAc                         >  7:245398/1‑149 (MQ=255)                                                                                                                    cGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                        <  7:57256/149‑1 (MQ=255)                                                                                                                    cGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                        >  2:270413/1‑149 (MQ=255)                                                                                                                     gAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                  >  3:242293/1‑107 (MQ=255)                                                                                                                     gAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                  <  4:242293/107‑1 (MQ=255)                                                                                                                     gAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                       <  7:75118/149‑1 (MQ=255)                                                                                                                           gCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATg                                                                                     <  4:192790/82‑1 (MQ=255)                                                                                                                           gCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATg                                                                                     >  3:192790/1‑82 (MQ=255)                                                                                                                           gCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACggg                      >  6:299709/1‑144 (MQ=255)                                                                                                                           gCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACggg                      <  5:299709/144‑1 (MQ=255)                                                                                                                                  gCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAAc                         <  1:19058/134‑1 (MQ=255)                                                                                                                                  gCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAAc                         >  2:19058/1‑134 (MQ=255)                                                                                                                                  gCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGa           >  2:16068/1‑148 (MQ=255)                                                                                                                                   ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                       <  4:66970/135‑1 (MQ=255)                                                                                                                                   ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                       >  3:66970/1‑135 (MQ=255)                                                                                                                                   ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGgatgat                 <  6:270855/141‑1 (MQ=255)                                                                                                                                   ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGgatgat                 <  4:314963/141‑1 (MQ=255)                                                                                                                                   ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGgatgat                 >  5:270855/1‑141 (MQ=255)                                                                                                                                   ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGgatgat                 >  3:314963/1‑141 (MQ=255)                                                                                                                                   ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGaaa         >  6:21869/1‑149 (MQ=255)                                                                                                                                   ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGaaa         >  4:282453/1‑149 (MQ=255)                                                                                                                                     tGGATTACGACCTGCGCCTGTATGCGCAGATAGTGGCGGAGCGCACGAAAGGCGCGGCCGAGCTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGCATGATCCATGAAATc       >  2:142269/1‑149 (MQ=255)                                                                                                                                       gATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcga     <  5:21869/149‑1 (MQ=255)                                                                                                                                        aTTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac    <  8:245398/149‑1 (MQ=255)                                                                                                                                         ccaCGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac    >  7:324329/3‑148 (MQ=255)                                                                                                                                            cGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCt                                >  5:353215/1‑117 (MQ=255)                                                                                                                                            cGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCt                                <  6:353215/117‑1 (MQ=255)                                                                                                                                            cGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                        >  7:276019/1‑125 (MQ=255)                                                                                                                                            cGATCTGCGCATACAGGCGCAGGTCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                        <  8:276019/125‑1 (MQ=255)                                                                                                                                            cGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCTCAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgacga  <  1:270413/148‑1 (MQ=255)                                                                                                                                            cGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac    >  2:16461/1‑145 (MQ=255)                                                                                                                                               |                                                                                                                                                 TGGGCAATATCCTCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTCCGAGTATGCGATGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCC‑AAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGA  >  NC_002947/3701836‑3702121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑