Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,703,068 A→G 8.9% intergenic (+56/‑109) PP_3269 → / → PP_5596 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,703,0680AG 8.9% 119.4 / 2.0 46intergenic (+56/‑109)PP_3269/PP_5596hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/19);  new base G (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

GCAACTTTGCCAACGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTACATAATCGAT  >  NC_002947/3702927‑3703206
                                                                                                                                             |                                                                                                                                          
gcAACTTTGCCAACGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGccc                                                                                                                                     >  3:89410/1‑149 (MQ=255)
     tttGCCAACGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGCCAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTCGCCGCCCCGGaa                                                                                                                                >  2:250208/1‑149 (MQ=255)
          cAACGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATcgcg                                                                                                                           >  2:235827/1‑149 (MQ=255)
           aaCGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCACCCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCgcgc                                                                                                                          >  1:214475/1‑149 (MQ=255)
                   ccAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCa                                                                                                                        <  6:232895/143‑1 (MQ=255)
                   ccAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCa                                                                                                                        >  5:232895/1‑143 (MQ=255)
                   ccAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCa                                                                                                                        >  5:238044/1‑143 (MQ=255)
                   ccAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCa                                                                                                                        <  6:238044/143‑1 (MQ=255)
                    cAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACAtt                                                                                                                 >  8:191182/1‑149 (MQ=255)
                          gCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                             >  3:145774/1‑147 (MQ=255)
                           cATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                             >  2:12077/1‑146 (MQ=255)
                           cATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                             >  1:290703/1‑146 (MQ=255)
                                 aaGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACaaaa                                                                                                    >  8:314013/1‑149 (MQ=255)
                                     cGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCCACCAGTGCGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTGCGGGGTATCCGCCACGGAATCGCGCCATGACATTTTGTCTAACAAAAtgtc                                                                                                <  2:214475/149‑4 (MQ=255)
                                     cGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAAtttt                                                                                                >  5:257401/1‑149 (MQ=255)
                                     cGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTCTAACAAAAtgtc                                                                                                <  4:89410/149‑4 (MQ=255)
                                           cAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTCTAACAAAAtgtc                                                                                                >  2:158945/1‑140 (MQ=255)
                                           cAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTCTAACAAAAtgtc                                                                                                <  1:158945/143‑4 (MQ=255)
                                              gggagggCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                             <  3:137589/127‑1 (MQ=255)
                                              gggagggCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                             >  4:137589/1‑127 (MQ=255)
                                               ggagggCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTg                                                                                      <  1:12077/149‑1 (MQ=255)
                                                  gggCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGcc                                                                                   <  7:314013/149‑1 (MQ=255)
                                                               aCGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGatcaa                                                                      >  3:126878/1‑149 (MQ=255)
                                                                      aGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCAt                                                                                                                       >  1:135950/1‑93 (MQ=255)
                                                                      aGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCAt                                                                                                                       <  2:135950/93‑1 (MQ=255)
                                                                      aGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAAt                                                               <  4:145774/149‑1 (MQ=255)
                                                                      aGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAAt                                                               <  7:191182/149‑1 (MQ=255)
                                                                             aaCCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATc                                                         >  3:74213/1‑148 (MQ=255)
                                                                                     aGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGc                                                                                    <  1:59214/113‑1 (MQ=255)
                                                                                     aGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGc                                                                                    >  2:59214/1‑113 (MQ=255)
                                                                                          gCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAagag                                             >  6:147975/1‑147 (MQ=255)
                                                                                            tttCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACAttt                                                                                                                <  4:304753/78‑1 (MQ=255)
                                                                                                gCTGAACGTCAGCTCCCGATAGGTGCCCTGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCTTGGTGAAGATGAGGcccc                                     <  2:290703/149‑1 (MQ=255)
                                                                                                       gACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGAt                              <  4:126878/149‑1 (MQ=255)
                                                                                                                    aGGTGCCCAGCAGCGATAACGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAAACTTCAGCCCACCGCCCGATCAATCAAAAAGTCATCGATCGAGAAGAGGAGGCCCCCTGGGATGACTAGCCCACAg                 >  6:194678/1‑149 (MQ=255)
                                                                                                                         cccAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGt             <  6:257401/148‑1 (MQ=255)
                                                                                                                          ccAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTAc           <  2:150183/149‑1 (MQ=255)
                                                                                                                           atggcgcGATTCCGGGGCGGCTACCCCGAAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCcaca                  >  4:238677/6‑141 (MQ=38)
                                                                                                                           atggcgcGATTCCGGGGCGGCTACCCCGAAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCcaca                  <  3:238677/136‑1 (MQ=38)
                                                                                                                                  gATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGcc                      >  3:225174/1‑130 (MQ=255)
                                                                                                                                  gATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGcc                      <  4:225174/130‑1 (MQ=255)
                                                                                                                                  gATTCCGGGGCGGCTACCCCGAAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGagaaga                                              >  2:23213/1‑106 (MQ=38)
                                                                                                                                  gATTCCGGGGCGGCTACCCCGAAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGagaaga                                              <  1:23213/106‑1 (MQ=38)
                                                                                                                                    tttCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTa            <  7:168101/138‑1 (MQ=255)
                                                                                                                                    tttCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTa            >  8:168101/1‑138 (MQ=255)
                                                                                                                                    tttCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTACATAATCGAt  >  3:213634/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                          
GCAACTTTGCCAACGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTACATAATCGAT  >  NC_002947/3702927‑3703206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑