Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,703,078 G→A 8.3% intergenic (+66/‑99) PP_3269 → / → PP_5596 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,703,0780GA 8.3% 135.1 / 2.1 48intergenic (+66/‑99)PP_3269/PP_5596hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/20);  new base A (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

TTTGCCAACGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTACATAATCGATTACAAACTGCATGAC  >  NC_002947/3702932‑3703221
                                                                                                                                                  |                                                                                                                                               
tttGCCAACGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGCCAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTCGCCGCCCCGGaa                                                                                                                                               >  2:250208/1‑149 (MQ=255)
     cAACGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATcgcg                                                                                                                                          >  2:235827/1‑149 (MQ=255)
      aaCGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCACCCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCgcgc                                                                                                                                         >  1:214475/1‑149 (MQ=255)
              ccAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCa                                                                                                                                       >  5:238044/1‑143 (MQ=255)
              ccAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCa                                                                                                                                       <  6:232895/143‑1 (MQ=255)
              ccAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCa                                                                                                                                       <  6:238044/143‑1 (MQ=255)
              ccAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCa                                                                                                                                       >  5:232895/1‑143 (MQ=255)
               cAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACAtt                                                                                                                                >  8:191182/1‑149 (MQ=255)
                     gCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                                            >  3:145774/1‑147 (MQ=255)
                      cATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                                            >  2:12077/1‑146 (MQ=255)
                      cATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                                            >  1:290703/1‑146 (MQ=255)
                            aaGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACaaaa                                                                                                                   >  8:314013/1‑149 (MQ=255)
                                cGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCCACCAGTGCGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTGCGGGGTATCCGCCACGGAATCGCGCCATGACATTTTGTCTAACAAAAtgtc                                                                                                               <  2:214475/149‑4 (MQ=255)
                                cGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAAtttt                                                                                                               >  5:257401/1‑149 (MQ=255)
                                cGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTCTAACAAAAtgtc                                                                                                               <  4:89410/149‑4 (MQ=255)
                                      cAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTCTAACAAAAtgtc                                                                                                               >  2:158945/1‑140 (MQ=255)
                                      cAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTCTAACAAAAtgtc                                                                                                               <  1:158945/143‑4 (MQ=255)
                                         gggagggCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                                            >  4:137589/1‑127 (MQ=255)
                                         gggagggCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGt                                                                                                                            <  3:137589/127‑1 (MQ=255)
                                          ggagggCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTg                                                                                                     <  1:12077/149‑1 (MQ=255)
                                             gggCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGcc                                                                                                  <  7:314013/149‑1 (MQ=255)
                                                          aCGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGatcaa                                                                                     >  3:126878/1‑149 (MQ=255)
                                                                 aGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCAt                                                                                                                                      >  1:135950/1‑93 (MQ=255)
                                                                 aGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCAt                                                                                                                                      <  2:135950/93‑1 (MQ=255)
                                                                 aGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAAt                                                                              <  4:145774/149‑1 (MQ=255)
                                                                 aGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAAt                                                                              <  7:191182/149‑1 (MQ=255)
                                                                        aaCCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATc                                                                        >  3:74213/1‑148 (MQ=255)
                                                                                aGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGc                                                                                                   <  1:59214/113‑1 (MQ=255)
                                                                                aGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGc                                                                                                   >  2:59214/1‑113 (MQ=255)
                                                                                     gCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAagag                                                            >  6:147975/1‑147 (MQ=255)
                                                                                       tttCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACAttt                                                                                                                               <  4:304753/78‑1 (MQ=255)
                                                                                           gCTGAACGTCAGCTCCCGATAGGTGCCCTGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCTTGGTGAAGATGAGGcccc                                                    <  2:290703/149‑1 (MQ=255)
                                                                                                  gACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGAt                                             <  4:126878/149‑1 (MQ=255)
                                                                                                               aGGTGCCCAGCAGCGATAACGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAAACTTCAGCCCACCGCCCGATCAATCAAAAAGTCATCGATCGAGAAGAGGAGGCCCCCTGGGATGACTAGCCCACAg                                >  6:194678/1‑149 (MQ=255)
                                                                                                                    cccAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGt                            <  6:257401/148‑1 (MQ=255)
                                                                                                                     ccAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTAc                          <  2:150183/149‑1 (MQ=255)
                                                                                                                      atggcgcGATTCCGGGGCGGCTACCCCGAAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCcaca                                 >  4:238677/6‑141 (MQ=38)
                                                                                                                      atggcgcGATTCCGGGGCGGCTACCCCGAAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCcaca                                 <  3:238677/136‑1 (MQ=38)
                                                                                                                             gATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGcc                                     >  3:225174/1‑130 (MQ=255)
                                                                                                                             gATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGcc                                     <  4:225174/130‑1 (MQ=255)
                                                                                                                             gATTCCGGGGCGGCTACCCCGAAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGagaaga                                                             >  2:23213/1‑106 (MQ=38)
                                                                                                                             gATTCCGGGGCGGCTACCCCGAAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGagaaga                                                             <  1:23213/106‑1 (MQ=38)
                                                                                                                               tttCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTa                           <  7:168101/138‑1 (MQ=255)
                                                                                                                               tttCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTa                           >  8:168101/1‑138 (MQ=255)
                                                                                                                               tttCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTACATAATCGAt                 >  3:213634/1‑148 (MQ=255)
                                                                                                                                         gccgccCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTACATAATCGATTACAAACTGc       <  4:213634/148‑1 (MQ=255)
                                                                                                                                             ccccGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTACATAATCGATTACAAACTGCATGAc  >  4:322350/1‑149 (MQ=255)
                                                                                                                                              cccGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTACATAATCGATTACAAACTGCATGAc  >  4:147326/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TTTGCCAACGACCGCCAACGGGCATCGGAAGCCGGTCGCAAGGGAGGGCGCAATAGCCACGGCGCAGGCCGCAACCAGTGAGGGGGCTTTCGCTGAACGACAGCTCCCCATAGGTGCCCAGCAGCGATTTCGGGGTAGCCGCCCCGGAATCGCGCCATGACATTTTGTTAGACAAAATTTTGAATCTTCTGGCCACCGCCCGATCAATCAAAATGTCATCCATGGAGAAGAGGAGGCCCCCTGCGATGCCTAGCCCACAGCTGTACATAATCGATTACAAACTGCATGAC  >  NC_002947/3702932‑3703221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑