Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,718,101 C→G 7.8% L164L (CTG→CTC)  paaG ← 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,718,1010CG 7.8% 196.6 / 6.8 64L164L (CTG→CTC) paaG2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base C (31/28);  new base G (2/3);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

TGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCA  >  NC_002947/3717960‑3718248
                                                                                                                                             |                                                                                                                                                    
tgtCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGCCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGGAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCACGCATGGCCAGCGCCt                                                                                                                                               >  2:43897/1‑149 (MQ=255)
       ccGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCg                                                                                                                                        >  7:221504/1‑149 (MQ=255)
        cGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGACCCCATTGCTCGGCCTGTTCGGCGACAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCgg                                                                                                                                       >  2:345484/1‑149 (MQ=255)
            ggggggctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCTCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCa                                                                                                                                   <  1:43897/146‑1 (MQ=255)
                    gggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGAc                                                                                                                            >  2:5402/1‑148 (MQ=255)
                    gggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGAc                                                                                                                            >  6:333506/1‑148 (MQ=255)
                         gcgAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAgcg                                                                                                                      <  5:333506/149‑1 (MQ=255)
                              gTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGACCAgcgc                                                                                                                                                 >  2:246249/1‑117 (MQ=255)
                              gTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTCCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGACCAgcgc                                                                                                                                                 <  1:246249/117‑1 (MQ=255)
                                      cgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCaa                                                                                                         <  3:212751/149‑1 (MQ=255)
                                           gTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCagcagc                                                                                                            <  1:344125/141‑1 (MQ=255)
                                           gTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCagcagc                                                                                                            >  2:344125/1‑141 (MQ=255)
                                            tGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCgg                                                                                                                                       <  3:27702/113‑1 (MQ=255)
                                            tGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCgg                                                                                                                                       >  4:27702/1‑113 (MQ=255)
                                             gAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAccgc                                                                                                  >  8:177089/1‑149 (MQ=255)
                                              aGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGACCCCATTGCTCGGCCTGTTCGGCGACAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTAccgcc                                                                                                 <  1:345484/149‑1 (MQ=255)
                                                  cTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAg                                                                                             >  8:148794/1‑149 (MQ=255)
                                                   ttCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCtt                                                                                                                                              <  2:9179/99‑1 (MQ=255)
                                                      atcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTcc                                                                                          <  7:148794/148‑1 (MQ=255)
                                                      atcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCACTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAgc                                                                                                                       <  6:260180/119‑1 (MQ=255)
                                                       tcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGcagcag                                                                                                             >  6:167119/1‑128 (MQ=255)
                                                       tcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGcagcag                                                                                                             <  5:167119/128‑1 (MQ=255)
                                                       tcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCgg                                                                                        >  4:69071/1‑149 (MQ=255)
                                                          cGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGcc                                                                                                                                    <  3:157188/102‑1 (MQ=255)
                                                           gCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                                    <  7:177089/149‑1 (MQ=255)
                                                                gcTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGgcag                                                                                                                >  8:69406/1‑116 (MQ=255)
                                                                   gCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGAt                                                                            >  7:304776/1‑149 (MQ=255)
                                                                     atcatcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCt                                                                          >  2:103041/1‑149 (MQ=255)
                                                                      tcatcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCtt                                                                         >  8:328823/1‑149 (MQ=255)
                                                                        atcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                                        >  4:316486/1‑148 (MQ=255)
                                                                            accacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAg                                                                     >  2:203496/1‑147 (MQ=255)
                                                                            accacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGaa                                                                   <  1:103041/149‑1 (MQ=255)
                                                                             ccacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAg                                                                  >  4:291101/1‑149 (MQ=255)
                                                                                             cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTgg                                                 <  3:69071/149‑1 (MQ=255)
                                                                                              ccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGc                                                <  7:328823/149‑1 (MQ=255)
                                                                                              ccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGc                                                <  1:203496/149‑1 (MQ=255)
                                                                                               cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGc                                                                       >  1:195893/1‑126 (MQ=255)
                                                                                               cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGc                                                                       <  2:195893/126‑1 (MQ=255)
                                                                                               cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGcc                                               <  1:5402/149‑1 (MQ=255)
                                                                                                ccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCg                                              >  7:151776/1‑149 (MQ=255)
                                                                                                 cATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCgc                                             >  2:77/1‑148 (MQ=255)
                                                                                                 cATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGa                                             <  3:316486/149‑1 (MQ=255)
                                                                                                     gCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCtt                                                                         <  8:112557/118‑1 (MQ=255)
                                                                                                     gCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCtt                                                                         >  7:112557/1‑118 (MQ=255)
                                                                                                          gCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGggcggc                                    <  8:221504/149‑1 (MQ=255)
                                                                                                           ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCACCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGcc                                   <  5:219507/149‑1 (MQ=255)
                                                                                                               ttCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCa                               >  5:358997/1‑149 (MQ=255)
                                                                                                                cccgggcCAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCGCCGAACAGGCCGAGCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCAc                              <  5:17644/144‑1 (MQ=21)
                                                                                                                 ccacctcAACGCGCTCGCCCCCCAGGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCCGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCa                               <  8:259645/141‑1 (MQ=255)
                                                                                                                  ggCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCAcc                             <  6:358997/148‑1 (MQ=255)
                                                                                                                    cgcCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAgg                          <  8:151776/149‑1 (MQ=255)
                                                                                                                     gcCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCg                                                                                         <  4:116478/86‑1 (MQ=255)
                                                                                                                     gcCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCg                                                                                         >  3:116478/1‑86 (MQ=255)
                                                                                                                        aaGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                       >  3:266484/1‑148 (MQ=255)
                                                                                                                        aaGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCa                               >  7:259645/1‑140 (MQ=255)
                                                                                                                          ggCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGCCCGAGCGGGCGGCCAGCACCAGGTCGCAg                    >  4:322667/1‑149 (MQ=255)
                                                                                                                           gcgcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAgg                   >  2:95629/1‑149 (MQ=255)
                                                                                                                              cTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGcc                 >  4:52456/1‑148 (MQ=255)
                                                                                                                                   ccAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTgg                                                 >  1:280929/1‑111 (MQ=255)
                                                                                                                                   ccAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTgg                                                 <  2:280929/111‑1 (MQ=255)
                                                                                                                                    cAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGaa          >  7:310910/1‑149 (MQ=255)
                                                                                                                                       cATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGt       >  4:156634/1‑149 (MQ=255)
                                                                                                                                          ggCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGtt      <  3:322667/147‑1 (MQ=255)
                                                                                                                                          ggCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTg     <  4:266484/148‑1 (MQ=255)
                                                                                                                                            ccAGCGCCTTGGCCCGGGCCATGACGACCAAGCGCGGCAGCAGCAAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTggca  >  7:220884/1‑149 (MQ=255)
                                                                                                                                             cAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGTATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTggca  <  1:95629/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
TGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGG‑ATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCA  >  NC_002947/3717960‑3718248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑