Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,769,985 C→T 9.3% G524G (GGC→GGT)  PP_3330 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,769,9850CT 9.3% 118.5 / 2.6 43G524G (GGC→GGT) PP_3330ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base C (17/22);  new base T (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CTCGACCGACGCTGGGACGCCATCACCGGTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCAGCGGCGTATCGCATCGTGCGCAAGGATATCTCCGTGCCCTCCTCGACCGTGCCCAA  >  NC_002947/3769842‑3770122
                                                                                                                                               |                                                                                                                                         
cTCGACCGACGCTGGGACGCCATCACCGGTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACtt                                                                                                                                      >  2:284852/1‑149 (MQ=255)
   gaccgacGCTGGGACGCCATCACCGGTCGCCTGGGCCTGGGCTATGACCAGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGa                                                                                                                                   <  5:351690/149‑1 (MQ=255)
    accgacGCTGGGACGCCATCACCGGTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGAc                                                                                                                                  <  1:161647/149‑1 (MQ=255)
    accgacGCTGGGACGCCATCACCGGTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGAc                                                                                                                                  <  8:195027/149‑1 (MQ=255)
       gacGCTGGGACGCCATCACCGGTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTg                                                                                                                               <  6:344978/149‑1 (MQ=255)
                  gCCATCACCGGTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCg                                                                                                                    >  6:143009/1‑149 (MQ=255)
                     aTCACCGGTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCa                                                                                                                 <  3:272396/149‑1 (MQ=255)
                        aCCGGTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAgg                                                                                                               <  6:86152/148‑1 (MQ=255)
                            gTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAg                                                                                                          <  5:143009/149‑1 (MQ=255)
                             tCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAgg                                                                                                         <  3:127726/149‑1 (MQ=255)
                                cctgggcctggTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAg                                                                                                      >  6:22582/1‑149 (MQ=255)
                                cctgggcctggTCTATGACCTGACCTCCAACGTAACCCTGTACACCCAATACAGCAACTAGGCCGAACCACCAGGCGGTAACCTGACCAGCGCATCGATCAGCCAGGTCGGCCAATTCGACATAACCACCGGCCGTCAGGTCGAGGTAg                                                                                                      >  7:52683/1‑149 (MQ=255)
                                       ctggTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGgcagc                                                                                                 >  3:135818/1‑147 (MQ=255)
                                          gTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTc                                                                                                                  >  7:23278/1‑127 (MQ=255)
                                          gTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTc                                                                                                                  <  8:23278/127‑1 (MQ=255)
                                           tCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTc                                                                                           <  5:22582/149‑1 (MQ=255)
                                            cTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAgg                                                                                                         >  6:239858/1‑134 (MQ=255)
                                            cTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAgg                                                                                                         <  5:239858/134‑1 (MQ=255)
                                             tATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGt                                                                                                        <  3:36629/134‑1 (MQ=255)
                                             tATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGt                                                                                                        >  4:36629/1‑134 (MQ=255)
                                               tgacctgaccTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGATCCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGa                                                                                         <  1:284852/147‑1 (MQ=255)
                                                    tgacctCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACt                                                                                       >  6:124844/1‑144 (MQ=255)
                                                    tgacctCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACt                                                                                       <  5:124844/144‑1 (MQ=255)
                                                     gacctCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTc                                                                                  >  5:218356/1‑148 (MQ=255)
                                                                   cccTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGTGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCg                                                                   >  4:39323/1‑149 (MQ=255)
                                                                        tACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGTGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGAc                                                              <  3:39323/149‑1 (MQ=255)
                                                                            cccAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCa                                                          <  4:135818/149‑1 (MQ=255)
                                                                             ccAATACAGCATCTCGGCCGAACCACCAGGCGGTAGCCTGACCATCGCCTCGATCAGCCTGGTCTGCGACTTCGACCTGAGc                                                                                                                            <  8:80689/82‑1 (MQ=255)
                                                                                   cAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTc                                                                                     >  2:37552/1‑115 (MQ=255)
                                                                                   cAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTc                                                                                     <  1:37552/115‑1 (MQ=255)
                                                                                   cAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCAgcggcg                                                    >  7:306289/1‑148 (MQ=255)
                                                                                          tCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTc                                                                                     <  3:90789/108‑1 (MQ=255)
                                                                                          tCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTc                                                                                     >  4:90789/1‑108 (MQ=255)
                                                                                               cGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACg                                                             <  2:196648/127‑1 (MQ=255)
                                                                                               cGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACg                                                             >  1:196648/1‑127 (MQ=255)
                                                                                                    caccaGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGAc                                                                               <  3:261905/104‑1 (MQ=255)
                                                                                                    caccaGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGAc                                                                               >  4:261905/1‑104 (MQ=255)
                                                                                                                  cTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCAGCGGCGTATCGCATCGTGCGCAAGGATATCTCCGTGcc                    <  6:218356/149‑1 (MQ=255)
                                                                                                                   tGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCAgcggc                                                     >  3:160988/1‑115 (MQ=255)
                                                                                                                            ccTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGCTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCAGCGGCGTATCGCATCGTGCGCAAGGATATCTCCGTGCCCTCCTCGAcc          >  1:130321/1‑149 (MQ=255)
                                                                                                                                    aGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCAGCGGCGTATCGCATCGTGCGCAAGGATATCTCCGTGCCCTCCTCGACCGTGCCCaa  <  8:306289/149‑1 (MQ=255)
                                                                                                                                        aGGTCGGTGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCAGCGGCGTATCGCATCGTGCGCAAGGATATCTCCGTGCcctcc                <  2:328027/131‑1 (MQ=255)
                                                                                                                                        aGGTCGGTGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCAGCGGCGTATCGCATCGTGCGCAAGGATATCTCCGTGCcctcc                >  1:328027/1‑131 (MQ=255)
                                                                                                                                               |                                                                                                                                         
CTCGACCGACGCTGGGACGCCATCACCGGTCGCCTGGGCCTGGTCTATGACCTGACCTCCAACGTCACCCTGTACACCCAATACAGCAACTCGGCCGAACCACCAGGCGGTACCCTGACCAGCGCCTCGATCAGCCAGGTCGGCGACTTCGACCTGAGCACCGGCCGTCAGGTCGAGGTAGGCAGCAAGTTCGACTTCCTCGACGGCCGCGGCTCGGCGACGGCAGCGGCGTATCGCATCGTGCGCAAGGATATCTCCGTGCCCTCCTCGACCGTGCCCAA  >  NC_002947/3769842‑3770122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑