Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,782,988	C→T	6.2%	Q27* (CAG→TAG)	nikD →	nickel ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,782,988	0	C	T	6.2%	189.4 / 2.2	64	Q27* (CAG→TAG)	nikD	nickel ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (32/28);  new base T (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

TGATGGCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAACTGCTGCTCGACGGCCAGCCGCTGGCTGCAAG  >  NC_002947/3782842‑3783136                                                                                                                                                   |                                                                                                                                                     tGATGGCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAg                                                                                                                                                    >  5:316677/1‑149 (MQ=255)     ggCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTgc                                                                                                                                                >  2:98411/1‑149 (MQ=255)      gCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGc                                                                                                                                                      <  8:23908/142‑1 (MQ=255)      gCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCaagatcg                                                                                                                                               >  7:23908/1‑143 (MQ=255)        cTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAACTCCGTGGCCTTCGCATAGAACCCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGcgcc                                                                                                                                             >  4:273686/1‑149 (MQ=255)            aaCCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCgcgg                                                                                                                                         <  5:31392/149‑1 (MQ=255)                tgctgGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGACTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCgcggcg                                                                                                                                       >  7:127749/1‑147 (MQ=255)                        gATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCAc                                                                                                                             >  8:46531/1‑149 (MQ=255)                              ctgcgcGACCGCCTGGACCCTGCACAGGAGCTCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGt                                                                                                                       <  1:98411/149‑1 (MQ=255)                               tgcgcGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTg                                                                                                                         <  1:49144/146‑1 (MQ=255)                               tgcgcGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTg                                                                                                                         >  2:49144/1‑146 (MQ=255)                                       cGCCTGGACCCTGCGCAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTgc                                                                                                                                                >  6:325527/1‑115 (MQ=255)                                       cGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAg                                                                                                              >  4:302432/1‑149 (MQ=255)                                        gCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGc                                                                                                             >  2:142531/1‑149 (MQ=255)                                          cTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCgg                                                                                                           <  1:142531/149‑1 (MQ=255)                                              aCCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGctct                                                                                                       <  1:312850/149‑1 (MQ=255)                                                ccTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTgg                                                                                                     >  3:148160/1‑149 (MQ=255)                                                ccTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTgg                                                                                                     <  6:316677/149‑1 (MQ=255)                                                ccTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTgg                                                                                                     <  1:254918/149‑1 (MQ=255)                                                  tGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGc                                                                                                    >  2:88057/1‑148 (MQ=255)                                                    cacaGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGc                                                                                                                 <  3:50108/133‑1 (MQ=255)                                                    cacaGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGc                                                                                                                 >  4:50108/1‑133 (MQ=255)                                                    cacaGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCaaa                                                                                                 >  8:285691/1‑149 (MQ=255)                                                     acaGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAAt                                                                                                <  4:148160/149‑1 (MQ=255)                                                           gcgcCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTgg                                                                                                     <  2:240733/138‑1 (MQ=255)                                                           gcgcCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTgg                                                                                                     >  1:240733/1‑138 (MQ=255)                                                                aTTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGc                                                                                     >  6:165894/1‑149 (MQ=255)                                                                 ttGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGcc                                                                                    <  5:179310/149‑1 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATc                                                                                               <  7:179307/135‑1 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATc                                                                                               >  8:179307/1‑135 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTc                                                                                           >  6:185084/1‑139 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTc                                                                                           <  5:185084/139‑1 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTg                                                                                 >  8:11904/1‑149 (MQ=255)                                                                    aaTGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTGGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGACGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTg                                                                                 <  2:36840/149‑1 (MQ=255)                                                                         gTCCGTCTGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGAGCCGCTGGCac                                                                                                  <  1:279151/127‑2 (MQ=255)                                                                         gTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGAGCCGCTGGCac                                                                                                  >  2:279151/1‑126 (MQ=255)                                                                                    cTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGc                                                                  <  5:65456/148‑1 (MQ=255)                                                                                      tGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGGCTTGTAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTac                                                               >  3:235069/1‑149 (MQ=255)                                                                                      tGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGGCTTGTAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTac                                                               >  1:89535/1‑149 (MQ=255)                                                                                       gAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTac                                                               <  1:310916/148‑1 (MQ=255)                                                                                         aaTCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTaccac                                                            >  1:249414/1‑149 (MQ=255)                                                                                           tCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAAt                                                                                                >  4:119988/1‑111 (MQ=255)                                                                                           tCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAAt                                                                                                <  7:171576/111‑1 (MQ=255)                                                                                           tCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAAt                                                                                                >  8:171576/1‑111 (MQ=255)                                                                                           tCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAAt                                                                                                <  3:119988/111‑1 (MQ=255)                                                                                            cggatctcTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCt                                                          <  7:245046/141‑1 (MQ=255)                                                                                                   cTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGAAGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGAGCTGCTACCACCTGGCCTCGc                                                  >  8:297681/1‑149 (MQ=255)                                                                                                    ttCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGACAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCt                                                          >  8:245046/1‑140 (MQ=255)                                                                                                       gCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCgg                                                                                                           <  2:16817/88‑1 (MQ=255)                                                                                                       gCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCgg                                                                                                           >  1:16817/1‑88 (MQ=255)                                                                                                          tAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCAcc                                           >  7:320278/1‑149 (MQ=255)                                                                                                             aaaCCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCaa                                        <  7:46531/149‑1 (MQ=255)                                                                                                              aaCCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCTGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAg                                       <  1:88057/149‑1 (MQ=255)                                                                                                                 ccAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAg                                       <  1:12929/146‑1 (MQ=255)                                                                                                                 ccAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAg                                       >  2:12929/1‑146 (MQ=255)                                                                                                                 ccAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGa                                     >  3:195220/1‑148 (MQ=255)                                                                                                                      ccGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCACAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAActg                               <  8:127749/149‑1 (MQ=255)                                                                                                                         gCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAActgctg                            >  8:242244/1‑149 (MQ=255)                                                                                                                              cTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAACTGCTGCTCGa                       <  4:195220/149‑1 (MQ=255)                                                                                                                                 gTGCATGACGTTGGCTTGTAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAACTGCTGCTCGACg                     <  2:89535/148‑1 (MQ=255)                                                                                                                                 gTGCATGACGTTGGCTTGTAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAACTGCTGCTCGACg                     <  4:235069/148‑1 (MQ=255)                                                                                                                                 gTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAACTGCTGCTCGACg                     <  2:249414/148‑1 (MQ=255)                                                                                                                                   gCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGt                                                                                                                   >  8:251721/1‑52 (MQ=255)                                                                                                                                           ttGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAACTGCTGCTCGACGGCCAGCCgctg          <  5:165894/149‑1 (MQ=255)                                                                                                                                                ttGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAACTGCTGCTCGACGGCCAGCCgctggctg      >  7:243218/1‑148 (MQ=255)                                                                                                                                                   cAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGc                                                  <  6:140146/101‑1 (MQ=255)                                                                                                                                                   cAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAACTGCTGCTCGACGGCCAGCCGCTGGCTGCAAg  <  7:242244/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     TGATGGCCTTCAACCTGCTGGGCGATGCGCTGCGCGACCGCCTGGACCCTGCACAGGAGCGCCATTGAATGAGTCCGTCGGTGCTTGAAATCCGTGGCCTTCGCATAGAAACCCAGGCCGGCGTGCTGGTGCATGACGTTGACTTGCAGCTGCGCCGCGGCGAAGTCTGCACGTTGGTCGGTGCCAGCGGCTCTGGCAAATCCCTCAGCTGCCTTGGCTTGCTCGACCTGCTACCACCTGGCCTCGCCCGCACCCAAGGACAACTGCTGCTCGACGGCCAGCCGCTGGCTGCAAG  >  NC_002947/3782842‑3783136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑