Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,882,380 T→A 6.3% F347I (TTC→ATC)  oprN → multidrug RND transporter outer membrane protein OprN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,882,3800TA 6.3% 190.9 / 2.7 63F347I (TTC→ATC) oprNmultidrug RND transporter outer membrane protein OprN
Reads supporting (aligned to +/- strand):  ref base T (33/26);  new base A (2/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGACAAGACCCAACAGCGTCTGCT  >  NC_002947/3882235‑3882528
                                                                                                                                                   |                                                                                                                                                    
cGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTGGCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCtt                                                                                                                                                     >  1:96114/1‑149 (MQ=255)
  ccAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGAc                                                                                                                                                 >  8:231090/1‑149 (MQ=255)
                 ccACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCgg                                                                                                                                  <  5:194320/149‑1 (MQ=255)
                           ccTGTTCCCGCGCGTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTgc                                                                                                                        >  3:93562/1‑149 (MQ=255)
                           ccTGTTCCCGCGCGTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTgc                                                                                                                        >  3:88681/1‑149 (MQ=255)
                              gTTCCCGCGCGTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGc                                                                                                                                    >  7:268471/1‑134 (MQ=255)
                              gTTCCCGCGCGTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGc                                                                                                                                    <  8:268471/134‑1 (MQ=255)
                               ttCCCGCGCGTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCgcgg                                                                                                                    >  8:179113/1‑149 (MQ=255)
                                    gcgcgTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCgcggc                                                                                                                   <  1:260138/145‑1 (MQ=255)
                                    gcgcgTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCgcggc                                                                                                                   >  2:260138/1‑145 (MQ=255)
                                           aGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGa                                                                                                        <  4:93562/149‑1 (MQ=255)
                                           aGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGa                                                                                                        >  6:323541/1‑149 (MQ=255)
                                           aGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGa                                                                                                        <  4:88681/149‑1 (MQ=255)
                                             ccTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATg                                                                                                      >  7:38043/1‑149 (MQ=255)
                                             ccTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGAGGGCCCGCCTGCGCGGCGCCAAGGCCGATg                                                                                                      >  5:231055/1‑149 (MQ=255)
                                              cTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGc                                                                                                     <  8:38043/149‑1 (MQ=255)
                                                 aGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCg                                                                                                   >  4:137835/1‑148 (MQ=255)
                                                   tGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAg                                                                                                <  3:137835/149‑1 (MQ=255)
                                                   tGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAg                                                                                                >  8:39051/1‑149 (MQ=255)
                                                     gCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAgg                                                                                               <  2:96114/148‑1 (MQ=255)
                                                      cTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAgggg                                                                                             <  7:39051/149‑1 (MQ=255)
                                                             tCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTg                                                                                        >  1:284774/1‑149 (MQ=255)
                                                               ggCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTg                                                                                        <  7:179113/147‑1 (MQ=255)
                                                                         ccgcccgcGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGaa                                                                            <  5:323541/149‑1 (MQ=255)
                                                                            cccgcGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAAcag                                                                         <  6:231055/149‑1 (MQ=255)
                                                                              cgcgGCTCGCAGATCGGCTCTTCGGCGGACAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCTACCTGGGCAGCGTGCGGGCCCTCCTGCGCTGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAAaagaa                                                                       <  7:313406/149‑3 (MQ=255)
                                                                                 ggCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGt                                                                    <  2:284774/149‑1 (MQ=255)
                                                                                      gCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGtgctgc                                                               >  3:138198/1‑149 (MQ=255)
                                                                                       cAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTgctgct                                                              <  4:138198/149‑1 (MQ=255)
                                                                                             ggCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCAGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACt                                                        >  6:252483/1‑149 (MQ=255)
                                                                                                tctTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTgg                                                      >  8:212675/1‑148 (MQ=255)
                                                                                                   tCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGaa                                                    >  1:82472/1‑147 (MQ=255)
                                                                                                   tCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGaa                                                    <  2:82472/147‑1 (MQ=255)
                                                                                                    cggcggCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAaga                                                  <  7:212675/148‑1 (MQ=255)
                                                                                                     ggcggcCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAaga                                                  <  3:38881/147‑1 (MQ=255)
                                                                                                     ggcggcCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAaga                                                  >  4:38881/1‑147 (MQ=255)
                                                                                                      gcggcCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTc                                               >  6:121437/1‑149 (MQ=255)
                                                                                                             aCGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCgg                                             <  7:84349/144‑1 (MQ=255)
                                                                                                             aCGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCgg                                             >  8:84349/1‑144 (MQ=255)
                                                                                                                  tGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTgg                                                      >  5:88399/1‑130 (MQ=255)
                                                                                                                  tGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTgg                                                      <  6:88399/130‑1 (MQ=255)
                                                                                                                      cACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGTGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGc                               <  7:231090/149‑1 (MQ=255)
                                                                                                                       aCTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGc                               >  7:330829/1‑148 (MQ=255)
                                                                                                                           ggCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTa                          <  5:121437/149‑1 (MQ=255)
                                                                                                                           ggCCCGAGCATTACCTGGGCGGCCATCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTa                          >  4:225147/1‑149 (MQ=255)
                                                                                                                           ggCCCGAGCATTACCTGGGCGGCCATCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAAAGCCTTCAGCGATTa                          >  4:229653/1‑149 (MQ=255)
                                                                                                                              ccGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGc                                      >  4:242133/1‑134 (MQ=255)
                                                                                                                              ccGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGc                                      <  3:242133/134‑1 (MQ=255)
                                                                                                                              ccGAGCATTACCTGGGCGGCCATCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGa                       <  3:225147/149‑1 (MQ=255)
                                                                                                                              ccGAGCATTACCTGGGCGGCCATCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGa                       <  3:229653/149‑1 (MQ=255)
                                                                                                                               cGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGCGCACTGGCCAACTACGAACAGCAGGTGCTTCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGAc                      <  5:252483/149‑1 (MQ=255)
                                                                                                                                gAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGc                                            <  6:213738/126‑1 (MQ=255)
                                                                                                                                gAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGc                                            >  5:213738/1‑126 (MQ=255)
                                                                                                                                gAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGAc                      >  8:227100/1‑148 (MQ=255)
                                                                                                                                gAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGAc                      >  1:133841/1‑148 (MQ=255)
                                                                                                                                gAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGAc                      >  5:21657/1‑148 (MQ=255)
                                                                                                                                gAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGAc                      >  8:8695/1‑148 (MQ=255)
                                                                                                                                    aTTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGACAAGAc                 >  7:143079/1‑149 (MQ=255)
                                                                                                                                        ccTGGGCGGCCTTCGTCCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGACAAGACCCaa             >  8:226319/1‑149 (MQ=255)
                                                                                                                                        ccTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGACAAGACCCaa             >  6:89068/1‑149 (MQ=255)
                                                                                                                                        ccTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGACAAGACCCaa             >  6:51392/1‑149 (MQ=255)
                                                                                                                                        ccTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGACAAGACCCaa             >  4:24030/1‑149 (MQ=255)
                                                                                                                                           gggCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCATCCGCGATTACGACAAGACCCAACAg          >  4:9585/1‑149 (MQ=255)
                                                                                                                                               ggcCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGTTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGACAAGACCCAACAGCGTc      <  7:227100/149‑1 (MQ=255)
                                                                                                                                                   ttCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGACAAGACCCAACAGCGTctgct  >  7:54498/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCCAACGTTGGCGTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTT‑‑CCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTGGGCGGCCTTCGACCTGGGCAGCGTGCGGGCCCGCCTGCGCGGCGCCAAGGCCGATGCCGAAGGGGCACTGGCCAACTACGAACAGCAGGTGCTGCTGGCACTGGAAGAGTCGGCCAACGCCTTCAGCGATTACGACAAGACCCAACAGCGTCTGCT  >  NC_002947/3882235‑3882528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑